Goat FBXL12 Polyclonal Antibody | anti-FBXL12 antibody

Goat anti-FBXL12 Antibody

Cat. Num. AAA420229

• Gene Names: FBXL12; Fbl12
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: FBXL12; Polyclonal Antibody; Goat anti-FBXL12 Antibody; likely ortholog of mouse f-box and leucine-rich repeat protein 12; FLJ20188; hypothetical protein FLJ20188; F-box and leucine-rich repeat protein 12; Fbl12; FBXL12 antibody; likely ortholog of mouse f-box and leucine-rich repeat protein 12 antibody; FLJ20188 antibody; hypothetical protein FLJ20188 antibody; F-box and leucine-rich repeat protein 12 antibody; Fbl12 antibody; anti-FBXL12 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: RACPKESMDWWM
• Sequence Length: 326

• Applicable Applications for anti-FBXL12 antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Lung shows staining of ciliated epithelial cells in the bronchioles. Recommended concentration, 4-6ug/ml.; Western Blot: Preliminary experiments gave no signal but low background in Human Brain lysates at up to 1ug/ml.
• Immunogen: Peptide with sequence RACPKESMDWWM, from the C Terminus of the protein sequence according to NP_060173.1.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((4ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 8923179
• NCBI GeneID: 54850
• NCBI Accession #: NP_060173.1
• NCBI GenBank Nucleotide #: NM_017703.2
• Molecular Weight: 30,491 Da
• NCBI Official Full Name: F-box/LRR-repeat protein 12 isoform a
• NCBI Official Synonym Full Names: F-box and leucine-rich repeat protein 12
• NCBI Official Symbol: FBXL12
• NCBI Official Synonym Symbols: Fbl12
• NCBI Protein Information: F-box/LRR-repeat protein 12
• UniProt Protein Name: F-box/LRR-repeat protein 12
• UniProt Gene Name: FBXL12
• UniProt Synonym Gene Names: FBL12
• UniProt Entry Name: FXL12_HUMAN

NCBI Description

Members of the F-box protein family, such as FBXL12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]

Uniprot Description

FBXL12: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; ubiquitin ligase complex

Molecular Function: protein binding

Biological Process: protein ubiquitination; ubiquitin-dependent protein catabolic process