Mouse Pan-QKI Monoclonal Antibody | anti-QKI antibody

Pan-QKI Monoclonal Antibody

Cat. Num. AAA191339

• Gene Names: QKI; QK; Hqk; QK1; QK3; hqkI
• Applications: Immunoblot, Western Blot, Immunofluorescence
• Synonyms: Pan-QKI; Monoclonal Antibody; Pan-QKI Monoclonal Antibody; anti-QKI antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Specificity: This antibody recognizes human, mouse, and rat QKI-5. It cross-reacts with QKI-6, QKI-7, and QKI-7b.
• Form/Format: 100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
• Sequence Length: 341

• Applicable Applications for anti-QKI antibody: Immunoblot, Immunofluorescence (IF)
• Application Notes: Immunoblot: 1-5ug/ml. A band of ~36-38kDa is detected.; IF: 10ug/ml; Dilute in PBS or medium that is identical to that used in the assay system.
• Immunogen: Fusion protein corresponding to aa 1-341 (full-length) of human QKI-5. This sequence is 100% identical to mouse, 99% identical to rat, and >90% identical to QKI-6, QKI-7, and QKI-7b.
• Preparation and Storage: This product is stable for at least one (1) year at-20 degree C.

Testing Data

(Detection of QKI-5 in rat brain lysate at 5ug/ml.)

Testing Data

(Detection of QKI-5 in neuroblastoma cell line SKN-BE at 10ug/ml: DAPI (blue) nuclear stain, Texas Red F actin stain, ATTO 488 (green) QKI-5 stain.)

Related Product Information for anti-QKI antibody

QKI (also called Protein Quaking or HqkI) is an RNA binding protein that plays a central role in myelinization. QKI acts by regulating pre-mRNA splicing, mRNA export, mRNA stability, and protein translation and is itself regulated by alternative splicing. QKI is expressed in the frontal cortex of brain; it is shown to be downregulated in the brains of schizophrenic patients.

NCBI and Uniprot Product Information

• NCBI GI #: 45827706
• NCBI GeneID: 9444
• NCBI Accession #: NP_006766.1
• NCBI GenBank Nucleotide #: NM_006775.2
• UniProt Accession #: Q96PU8; Q2I375; Q5MJQ1; Q969L9; Q96EJ3; Q96KA3; Q96PU6; Q96PU7; Q9P0X6; Q9P0X7; Q9P0X8; Q9P0X9
• Molecular Weight: 36,926 Da
• NCBI Official Full Name: protein quaking isoform 1
• NCBI Official Synonym Full Names: QKI, KH domain containing RNA binding
• NCBI Official Symbol: QKI
• NCBI Official Synonym Symbols: QK; Hqk; QK1; QK3; hqkI
• NCBI Protein Information: protein quaking
• UniProt Protein Name: Protein quaking
• Protein Family: Protein quaking
• UniProt Gene Name: QKI
• UniProt Synonym Gene Names: HKQ; Hqk; HqkI

NCBI Description

The protein encoded by this gene is an RNA-binding protein that regulates pre-mRNA splicing, export of mRNAs from the nucleus, protein translation, and mRNA stability. The encoded protein is involved in myelinization and oligodendrocyte differentiation and may play a role in schizophrenia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor ().