Rabbit anti-Human, Mouse JMJD3 Polyclonal Antibody | anti-JMJD3 antibody

JMJD3, ID (Kdm6b, Jmjd3, Kiaa0346, Lysine-specific demethylase 6B, JmjC domain-containing protein 3, Jumonji domain-containing protein 3) (Azide free) (HRP)

Cat. Num. AAA6311723

• Gene Names: Kdm6b; Jmjd3; BC038313; mKIAA0346; 1700064E03Rik
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: JMJD3; Polyclonal Antibody; ID (Kdm6b; Jmjd3; Kiaa0346; Lysine-specific demethylase 6B; JmjC domain-containing protein 3; Jumonji domain-containing protein 3) (Azide free) (HRP); Kdm6b; Jumonji domain-containing protein 3; anti-JMJD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-JMJD3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 964~993 amino acids from the center region of mouse JMJD3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-JMJD3 Center Pab (AP1022c) in Hela cell line lysates. JMJD3 Center(arrow) was detected using the purified Pab.)

Related Product Information for anti-JMJD3 antibody

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Product Categories/Family for anti-JMJD3 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 62945394
• NCBI GeneID: 216850
• NCBI Accession #: NP_001017426.1
• NCBI GenBank Nucleotide #: NM_001017426.1
• UniProt Accession #: Q5NCY0; Q3UWY9; Q4VC26; Q6ZQD3
• Molecular Weight: 176,355 Da
• NCBI Official Full Name: lysine-specific demethylase 6B
• NCBI Official Synonym Full Names: KDM1 lysine (K)-specific demethylase 6B
• NCBI Official Symbol: Kdm6b
• NCBI Official Synonym Symbols: Jmjd3; BC038313; mKIAA0346; 1700064E03Rik
• NCBI Protein Information: lysine-specific demethylase 6B
• UniProt Protein Name: Lysine-specific demethylase 6B
• UniProt Gene Name: Kdm6b
• UniProt Synonym Gene Names: Jmjd3; Kiaa0346

Uniprot Description

Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (PubMed:21095589).