Rabbit anti-Human JMJD1B Polyclonal Antibody | anti-JMJD1B antibody

JMJD1B, Phosphorylated (S291) (KDM3B, C5orf7, JHDM2B, JMJD1B, KIAA1082, Lysine-specific demethylase 3B, JmjC domain-containing histone demethylation protein 2B, Jumonji domain-containing protein 1B, Nuclear protein 5qNCA) (PE)

Cat. Num. AAA6311641

• Gene Names: KDM3B; 5qNCA; NET22; C5orf7; JMJD1B
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: JMJD1B; Polyclonal Antibody; Phosphorylated (S291) (KDM3B; C5orf7; JHDM2B; KIAA1082; Lysine-specific demethylase 3B; JmjC domain-containing histone demethylation protein 2B; Jumonji domain-containing protein 1B; Nuclear protein 5qNCA) (PE); KDM3B; Nuclear protein 5qNCA; anti-JMJD1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-JMJD1B antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Phospho-JMJD1B-pS291 Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected surrounding S291 of human JMJD1B.
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-JMJD1B-pS291 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-JMJD1B antibody

Antibodies; Enzymes; Demethylase

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Yamane, K., et al. Cell 125(3):483-495(2006). Andersen, J.S., et al. Nature 433(7021):77-83(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 38372909
• NCBI GeneID: 51780
• NCBI Accession #: NP_057688.2
• NCBI GenBank Nucleotide #: NM_016604.3
• UniProt Accession #: Q7LBC6; Q9BVH6; Q9BW93; Q9BZ52; Q9NYF4; Q9UPS0; A6H8X7
• Molecular Weight: 84,712 Da
• NCBI Official Full Name: lysine-specific demethylase 3B
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 3B
• NCBI Official Symbol: KDM3B
• NCBI Official Synonym Symbols: 5qNCA; NET22; C5orf7; JMJD1B
• NCBI Protein Information: lysine-specific demethylase 3B; nuclear protein 5qNCA; jumonji domain containing 1B; jumonji domain-containing protein 1B; jmjC domain-containing histone demethylation protein 2B
• UniProt Protein Name: Lysine-specific demethylase 3B
• UniProt Gene Name: KDM3B
• UniProt Synonym Gene Names: C5orf7; JHDM2B; JMJD1B; KIAA1082
• UniProt Entry Name: KDM3B_HUMAN

Uniprot Description

JMJD1B: a ubiquitous protein demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity. Three alternatively spliced isoforms have been reported.

Protein type: EC 1.14.11.-; Oxidoreductase; Demethylase

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm

Molecular Function: dioxygenase activity; metal ion binding

Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; chromatin modification