Western Blot (WB)
(Western blot analysis of JIP4 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse JIP4 Polyclonal Antibody | anti-JIP4 antibody
JIP4 Antibody
Gene Names
SPAG9; JLP; CT89; HLC4; HLC6; JIP4; PHET; PIG6; HLC-6; JIP-4
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
JIP4; Polyclonal Antibody; JIP4 Antibody; C jun amino terminal kinase interacting protein 4; c Jun NH2 terminal kinase associated leucine zipper protein; FLJ13450; FLJ14006; FLJ34602; HLC 4; HLC 4 protein; HLC 6; HLC4; HLC4 protein; HLC6; HSS; Human lung cancer protein 6; JIP 4; JNK associated leucine zipper protein 1; JNK associated leucine zipper protein; JNK interacting protein 4; JNK interacting protein; JNK/SAPK associated protein; KIAA0516; Lung cancer oncogene 4; MAPK8IP4; Max binding protein; MGC117291; MGC14967; MGC74461; Mitogen activated protein kinase 8 interacting protein 4; PHET; PIG 6; PIG6; Proliferation inducing gene 6; Protein highly expressed in testis; SPAG 9; SPAG9; Sperm associated antigen 9; Sperm specific protein; Sperm surface protein; Sunday driver (drosophila) homolog; Sunday driver 1; SYD 1; SYD1; anti-JIP4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
JIP4 antibody detects endogenous levels of total JIP4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1311
Applicable Applications for anti-JIP4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Cytoplasm, Perinuclear Region. Note: Perinuclear distribution in response to stress signals such as UV radiation.
Tissue Specificity
Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis. Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%), Chicken (91%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of JIP4 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of JIP4 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-JIP4 antibody
Function: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg-interaction.
Subunit Structure: Homodimer. The homodimer interacts with ARF6, forming a heterotetramer. Homooligomer. Interacts with MAX, MAPK8, MAPK9, MAPK10, MAPK14, MAP3K3, MYC, KNS2 and MAP2K4. Interaction with KNS2 is important in the formation of ternary complex with MAPK8. Interacts with NFKB1.
Post-translational Modifications: Phosphorylated by MAPK8 and MAPK14.
Similarity: Belongs to the JIP scaffold family.
Subunit Structure: Homodimer. The homodimer interacts with ARF6, forming a heterotetramer. Homooligomer. Interacts with MAX, MAPK8, MAPK9, MAPK10, MAPK14, MAP3K3, MYC, KNS2 and MAP2K4. Interaction with KNS2 is important in the formation of ternary complex with MAPK8. Interacts with NFKB1.
Post-translational Modifications: Phosphorylated by MAPK8 and MAPK14.
Similarity: Belongs to the JIP scaffold family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 156.8 kDa
Predicted: 147 kDa
Predicted: 147 kDa
NCBI Official Full Name
C-Jun-amino-terminal kinase-interacting protein 4 isoform 2
NCBI Official Synonym Full Names
sperm associated antigen 9
NCBI Official Symbol
SPAG9
NCBI Official Synonym Symbols
JLP; CT89; HLC4; HLC6; JIP4; PHET; PIG6; HLC-6; JIP-4
NCBI Protein Information
C-Jun-amino-terminal kinase-interacting protein 4
UniProt Protein Name
C-Jun-amino-terminal kinase-interacting protein 4
UniProt Gene Name
SPAG9
UniProt Synonym Gene Names
HSS; KIAA0516; MAPK8IP4; SYD1; JIP-4; JNK-interacting protein 4; CT89; HLC-6; JLP; PHET
NCBI Description
This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]
Uniprot Description
The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg-interaction.
Research Articles on JIP4
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Product Notes
The JIP4 spag9 (Catalog #AAA9603864) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JIP4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's JIP4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the JIP4 spag9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JIP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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