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Immunohistochemistry (IHC)
(MBS9604722 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit JIP2 Polyclonal Antibody | anti-JIP2 antibody
JIP2 Antibody
Gene Names
MAPK8IP2; IB2; IB-2; JIP2; PRKM8IPL
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
JIP2; Polyclonal Antibody; JIP2 Antibody; C jun amino terminal kinase interacting protein 2; C-jun-amino-terminal kinase-interacting protein 2; Homologous to mouse JIP 1; IB 2; IB-2; IB2; Islet brain 2; Islet-brain-2; JIP 2; JIP-2; JIP2_HUMAN; JNK interacting protein 2; JNK MAP kinase scaffold protein 2; JNK MAP kinase scaffold protein JIP2; JNK-interacting protein 2; MAPK8IP2; Mitogen activated protein kinase 8 interacting protein 2; Mitogen-activated protein kinase 8-interacting protein 2; PRKM8 interacting protein like; PRKM8IPL; anti-JIP2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
JIP2 antibody detects endogenous levels of total JIP2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
824
Applicable Applications for anti-JIP2 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Accumulates in cell surface projections.
Tissue Specificity
Expressed mainly in the brain and pancreas, including insulin-secreting cells. In the nervous system, more abundantly expressed in the cerebellum, pituitary gland, occipital lobe and the amygdala. Also expressed in fetal brain. Very low levels found in uterus, ovary, prostate, colon, testis, adrenal gland, thyroid gland and salivary gland.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Dog, Xenopus
Similarity
Pig (100%), Bovine (100%), Sheep (100%), Dog (91%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9604722 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604722 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunofluorescene (IF)
(MBS9604722 staining U87 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9604722 staining U87 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain cells, using JIP2 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain cells, using JIP2 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using JIP2 Antibody. Lane 1: HepG2 treated with antigen-specific peptide; Lane 2: HepG2; Lane 3: hela; )
Western Blot (WB)
(Western blot analysis of extracts from various samples, using JIP2 Antibody.
Lane 1: HepG2 treated with antigen-specific peptide;
Lane 2: HepG2;
Lane 3: hela;
)
Related Product Information for anti-JIP2 antibody
Function: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity).
Subunit Structure: Forms homo-or heterooligomeric complexes. Binds specific components of the JNK signaling pathway namely JNK1, JNK2, JNK3, MAP2K7, MAP3K10, MAP3K11, MAP3K12 and MAPK13. Also binds the proline-rich domain-containing splice variant of apolipoprotein E receptor 2 (ApoER2). Binds the cytoplasmic tails of LRP1 and LRP2 (Megalin). Binds the TPR motif-containing C-terminal of kinesin light chain, Klc1, pre-assembled MAPK8IP1 scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location (By similarity). Interacts with the cytoplasmic domain of APP (By similarity). Interacts with DCLK2. Interacts with TIAM1 and TIAM2 (By similarity). Interacts with FGF13; enables the interaction with MAPK13 and may regulate the MAPK8IP2 scaffolding activity.
Similarity: Belongs to the JIP scaffold family.
Subunit Structure: Forms homo-or heterooligomeric complexes. Binds specific components of the JNK signaling pathway namely JNK1, JNK2, JNK3, MAP2K7, MAP3K10, MAP3K11, MAP3K12 and MAPK13. Also binds the proline-rich domain-containing splice variant of apolipoprotein E receptor 2 (ApoER2). Binds the cytoplasmic tails of LRP1 and LRP2 (Megalin). Binds the TPR motif-containing C-terminal of kinesin light chain, Klc1, pre-assembled MAPK8IP1 scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location (By similarity). Interacts with the cytoplasmic domain of APP (By similarity). Interacts with DCLK2. Interacts with TIAM1 and TIAM2 (By similarity). Interacts with FGF13; enables the interaction with MAPK13 and may regulate the MAPK8IP2 scaffolding activity.
Similarity: Belongs to the JIP scaffold family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 87 kD
Predicted: 88 kDa
Predicted: 88 kDa
NCBI Official Full Name
C-Jun-amino-terminal kinase-interacting protein 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase 8 interacting protein 2
NCBI Official Symbol
MAPK8IP2
NCBI Official Synonym Symbols
IB2; IB-2; JIP2; PRKM8IPL
NCBI Protein Information
C-Jun-amino-terminal kinase-interacting protein 2
UniProt Protein Name
C-Jun-amino-terminal kinase-interacting protein 2
UniProt Gene Name
MAPK8IP2
UniProt Synonym Gene Names
IB2; JIP2; PRKM8IPL; JIP-2; JNK-interacting protein 2; IB-2
NCBI Description
This gene encodes a scaffold protein that is thought to be involved in the regulation of the c-Jun amino-terminal kinase signaling pathway. This protein has been shown to interact with and regulate the activity of MAPK8/JNK1 and MAP2K7/MKK7 kinases. [provided by RefSeq, Jun 2017]
Uniprot Description
The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins ().
Research Articles on JIP2
Similar Products
Product Notes
The JIP2 mapk8ip2 (Catalog #AAA9604722) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JIP2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's JIP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the JIP2 mapk8ip2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JIP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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