Rabbit Jip1 Polyclonal Antibody | anti-Mapk8ip1 antibody

Jip1 (C-Jun-amino-terminal Kinase-interacting Protein 1, JIP-1, JNK-interacting Protein 1, Islet-brain-1, IB-1, JNK MAP Kinase Scaffold Protein 1, Mitogen-activated Protein Kinase 8-interacting Protein 1, Mapk8ip1, Ib1, Mapk8ip, Prkm8ip)

Cat. Num. AAA641865

• Gene Names: Mapk8ip1; JIP1; Jip-1; Mapk8ip
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Jip1; Polyclonal Antibody; Jip1 (C-Jun-amino-terminal Kinase-interacting Protein 1; JIP-1; JNK-interacting Protein 1; Islet-brain-1; IB-1; JNK MAP Kinase Scaffold Protein 1; Mitogen-activated Protein Kinase 8-interacting Protein 1; Mapk8ip1; Ib1; Mapk8ip; Prkm8ip); Anti -Jip1 (C-Jun-amino-terminal Kinase-interacting Protein 1; anti-Mapk8ip1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse JIP-1 at ~78kD and ~110kD. Species Crossreactivity: human and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.

• Applicable Applications for anti-Mapk8ip1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-4ug/ml
• Immunogen: Synthetic peptide corresponding to mouse JIP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of JIP-1 in Jurkat cell lysate (Lane 1), mouse intestine (Lane 2) and rat kidney (Lane 3) tissue lysates.)

Related Product Information for anti-Mapk8ip1 antibody

JIP1 (JNK-interacting protein-1) is a cytoplasmic protein that possesses an N-terminal JNK-binding domain and a C-terminal SRC homology 3 (SH3) domain. JIP1 selectively bind to JNK, but not to the ERK or p38 MAP kinases. In addition to JNK, JIP1 binds to specific proteins of JNK pathways, including MKK7, MLK3, and DLK, all at separate binding sites. JIP1 has been identified in brain and kidney tissues, whereas JIP2a, JIP2b, and JIP3 are specifically expressed in the brain. Overexpression of JIP1 causes cytoplamic retention of JNK, thereby preventing its nuclear accumulation and the subsequent activation of the JNK pathways, indicating that JIP1 acts as a cytoplasmic anchor for JNK.

Product Categories/Family for anti-Mapk8ip1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 87130877
• NCBI GeneID: 116457
• UniProt Accession #: Q9R237; O88979; Q9R1H8; Q9WVI5; Q9WVI6
• Molecular Weight: 77,318 Da
• NCBI Official Full Name: JIP1 protein
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8 interacting protein 1
• NCBI Official Symbol: Mapk8ip1
• NCBI Official Synonym Symbols: JIP1; Jip-1; Mapk8ip
• NCBI Protein Information: C-Jun-amino-terminal kinase-interacting protein 1; JRP; IB-1; islet-brain-1; JIP-1-related protein; JNK-interacting protein 1; JNK MAP kinase scaffold protein 1; mitogen activated protein kinase 8 interacting protein; mitogen-activated protein kinase 8-interacting protein 1
• UniProt Protein Name: C-Jun-amino-terminal kinase-interacting protein 1
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: Mapk8ip1
• UniProt Synonym Gene Names: Ib1; Jip1; Mapk8ip; JIP-1; JNK-interacting protein 1; IB-1; JRP
• UniProt Entry Name: JIP1_RAT

NCBI Description

may play a role in the SAPK mediated stress-induced cellular responses [RGD, Feb 2006]

Uniprot Description

JIP1: a member of the JNK-interacting protein group of scaffold proteins that selectively mediates JNK signaling by aggregating specific components of the MAPK cascade. Required for JNK activation in response to excitotoxic stress. Causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and by inhibiting the JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly or indirectly regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interations with the JNK-signaling components and motor proteins.

Protein type: Adaptor/scaffold

Cellular Component: endoplasmic reticulum membrane; neuron projection; membrane; perinuclear region of cytoplasm; axon; dendrite; mitochondrial membrane; cytoplasm; synapse; nucleus; cytosol

Molecular Function: JUN kinase binding; identical protein binding; protein binding; MAP-kinase scaffold activity; mitogen-activated protein kinase kinase binding; kinesin binding; mitogen-activated protein kinase kinase kinase binding; protein kinase binding

Biological Process: vesicle-mediated transport; negative regulation of JNK activity; negative regulation of JNK cascade; positive regulation of signal transduction; regulation of transcription, DNA-dependent; signal transduction; regulation of JNK cascade; JUN phosphorylation; negative regulation of apoptosis