Rabbit JIP1 Polyclonal Antibody | anti-JIP1 antibody

JIP1 (Phospho-Thr103) Antibody

Cat. Num. AAA9508621

• Gene Names: MAPK8IP1; IB1; JIP1; JIP-1; PRKM8IP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: JIP1; Polyclonal Antibody; JIP1 (Phospho-Thr103) Antibody; C-jun-amino-terminal kinase interacting protein 1; IB-1; IB1; Islet-brain-1; JIP-1; JIP-1 related protein; JNK MAP kinase scaffold protein 1; JNK-interacting protein 1; JRP; MAPK8IP; MAPK8IP1; Mitogen-activated protein kinase 8-interacting protein 1; PRKM; anti-JIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: JIP1 (Phospho-Thr103) Antibody detects endogenous levels of MAPK8IP1 only when phosphorylated at Thr103.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 711

• Applicable Applications for anti-JIP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:T103; M:T103; R:T103
• Immunogen: The antiserum was produced against synthesized phospho-peptide derived from human JIP1 around the phosphorylation site of Thr103.
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using JIP1 (Phospho-Thr103) Antibody)

Western Blot (WB)

(Western blot analysis of lysates from COLO205 cells treated with Serum 20% 15', using JIP1 (Phospho-Thr103) Antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4885433
• NCBI GeneID: 9479
• NCBI Accession #: NP_005447.1
• NCBI GenBank Nucleotide #: NM_005456.3
• UniProt Accession #: Q9UQF2; O43407; D3DQP4
• Molecular Weight: 77 kDa
• NCBI Official Full Name: C-Jun-amino-terminal kinase-interacting protein 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8 interacting protein 1
• NCBI Official Symbol: MAPK8IP1
• NCBI Official Synonym Symbols: IB1; JIP1; JIP-1; PRKM8IP
• NCBI Protein Information: C-Jun-amino-terminal kinase-interacting protein 1
• UniProt Protein Name: C-Jun-amino-terminal kinase-interacting protein 1
• UniProt Gene Name: MAPK8IP1
• UniProt Synonym Gene Names: IB1; JIP1; PRKM8IP; JIP-1; JNK-interacting protein 1; IB-1

NCBI Description

This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. [provided by RefSeq, May 2011]

Uniprot Description

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (). Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response.