Rabbit anti-Human CD19 Polyclonal Antibody | anti-CD19 antibody

CD19 (B Lymphocyte Antigen CD19, Differentiation Antigen CD19, B4, CVID3) (FITC)

Cat. Num. AAA6410131

• Gene Names: CD19; B4; CVID3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: CD19; Polyclonal Antibody; CD19 (B Lymphocyte Antigen CD19; Differentiation Antigen CD19; B4; CVID3) (FITC); anti-CD19 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CD19.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-CD19 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human CD19.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CD19 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human smooth muscle cancer using CD19 antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human thyroid cancer using CD19 antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human breast cancer using CD19 antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded rat kidney using CD19 antibody at dilution of 1:200 (400x lens).)

Product Categories/Family for anti-CD19 antibody

Antibodies; CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 296010921
• NCBI GeneID: 930
• NCBI Accession #: NP_001171569.1
• NCBI GenBank Nucleotide #: NM_001178098.1
• UniProt Accession #: P15391; Q96S68; Q9BRD6; A0N0P9
• Molecular Weight: 61,128 Da
• NCBI Official Full Name: B-lymphocyte antigen CD19 isoform 1
• NCBI Official Synonym Full Names: CD19 molecule
• NCBI Official Symbol: CD19
• NCBI Official Synonym Symbols: B4; CVID3
• NCBI Protein Information: B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4
• UniProt Protein Name: B-lymphocyte antigen CD19
• Protein Family: B-lymphocyte antigen
• UniProt Gene Name: CD19
• UniProt Entry Name: CD19_HUMAN

NCBI Description

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]

Uniprot Description

CD19: a cell surface molecule which assembles with the antigen receptor of B lymphocytes. A critical signal transduction molecule that regulates B lymphocyte development, activation, and differentiation. Ligation increases intracellular calcium levels and decreases the threshold for antigen receptor signaling. Germinal center formation is significantly reduced in CD19-deficient mice.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: protein complex; integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: receptor signaling protein activity

Biological Process: epidermal growth factor receptor signaling pathway; B cell receptor signaling pathway; regulation of immune response; cell surface receptor linked signal transduction; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; cellular defense response; innate immune response; positive regulation of release of sequestered calcium ion into cytosol

Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 3