Rabbit anti-Human JHDM2b Polyclonal Antibody | anti-JHDM2b antibody
JHDM2b, ID (KDM3B, C5orf7, JHDM2B, JMJD1B, KIAA1082, Lysine-specific demethylase 3B, JmjC domain-containing histone demethylation protein 2B, Jumonji domain-containing protein 1B, Nuclear protein 5qNCA) (PE)
Gene Names
KDM3B; 5qNCA; NET22; C5orf7; JMJD1B
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
JHDM2b; Polyclonal Antibody; ID (KDM3B; C5orf7; JHDM2B; JMJD1B; KIAA1082; Lysine-specific demethylase 3B; JmjC domain-containing histone demethylation protein 2B; Jumonji domain-containing protein 1B; Nuclear protein 5qNCA) (PE); KDM3B; Nuclear protein 5qNCA; anti-JHDM2b antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-JHDM2b antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
JHDM2b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 876-905 amino acids from the Central region of human JHDM2b.
Conjugate
PE
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(JHDM2b Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the JHDM2b antibody detected the JHDM2b protein (arrow).)
Flow Cytometry (FC/FACS)
(JHDM2b Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-JHDM2b antibody
Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
Product Categories/Family for anti-JHDM2b antibody
References
Rose, J. Phd, et al. Mol. Med. (2010). In press: Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Yamane, K., et al. Cell 125(3):483-495(2006). Andersen, J.S., et al. Nature 433(7021):77-83(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,712 Da
NCBI Official Full Name
lysine-specific demethylase 3B
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 3B
NCBI Official Symbol
KDM3B
NCBI Official Synonym Symbols
5qNCA; NET22; C5orf7; JMJD1B
NCBI Protein Information
lysine-specific demethylase 3B; nuclear protein 5qNCA; jumonji domain containing 1B; jumonji domain-containing protein 1B; jmjC domain-containing histone demethylation protein 2B
UniProt Protein Name
Lysine-specific demethylase 3B
UniProt Gene Name
KDM3B
UniProt Synonym Gene Names
C5orf7; JHDM2B; JMJD1B; KIAA1082
UniProt Entry Name
KDM3B_HUMAN
Uniprot Description
JMJD1B: a ubiquitous protein demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity. Three alternatively spliced isoforms have been reported.
Protein type: EC 1.14.11.-; Oxidoreductase; Demethylase
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: nucleoplasm
Molecular Function: dioxygenase activity; metal ion binding
Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; chromatin modification
