Goat JARID2 Polyclonal Antibody | anti-JARID2 antibody

Goat anti-JARID2 Antibody

Cat. Num. AAA421634

• Gene Names: JARID2; JMJ
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: JARID2; Polyclonal Antibody; Goat anti-JARID2 Antibody; jumonji; AT rich interactive domain 2; JMJ; OTTHUMP00000016058; jumonji homolog; AT rich interactive domain 2 protein; jumonji-like protein; JARID2 antibody; AT rich interactive domain 2 antibody; JMJ antibody; OTTHUMP00000016058 antibody; jumonji homolog antibody; AT rich interactive domain 2 protein antibody; jumonji-like protein antibody; anti-JARID2 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: HRSAQDLRKQVSKVN
• Sequence Length: 1246

• Applicable Applications for anti-JARID2 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Preliminary experiments gave an approx 29kDa band in Mouse and Rat Spinal Cord and in Mouse Fetal Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137kDa according to Mouse NP_068678.1. The 29kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-HRSAQDLRKQVSKVN, from the internal region of the protein sequence according to NP_004964.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 11863152
• NCBI GeneID: 3720
• NCBI Accession #: NP_004964.2
• NCBI GenBank Nucleotide #: NM_004973.3
• Molecular Weight: 119,689 Da
• NCBI Official Full Name: protein Jumonji isoform 1
• NCBI Official Synonym Full Names: jumonji and AT-rich interaction domain containing 2
• NCBI Official Symbol: JARID2
• NCBI Official Synonym Symbols: JMJ
• NCBI Protein Information: protein Jumonji
• UniProt Protein Name: Protein Jumonji
• UniProt Gene Name: JARID2
• UniProt Synonym Gene Names: JMJ
• UniProt Entry Name: JARD2_HUMAN

NCBI Description

This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies. [provided by RefSeq, May 2012]

Uniprot Description

JARID2: a Polycomb protein required for neural tube formation. Contains a Jumonji C domain but lacks histone demethylase activity. Essential for normal heart development and function. Required for embryonic viability. Down-regulation of Jarid2 impairs ES cell differentiation and Oct4 silencing. Associates with the Polycomb-Repressive Complex 2 (PRC2), stimulating PRC2 methyltransferase activity. PRC2 includes the histone H3 Lys 27 (H3K27) methyltransferase Ezh2, EED, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The recruitment of PRC2 to a promoter leads to increased levels of di- and trimethylation of H3K27 (H3K27me2/3), regulating the balance between self-renewal and differentiation of embryonic stem cells. The ability of Jarid 2 to be recruited to DNA in ES cells is interdependent on PRC2. Its knockdown reduces PRC2 at its target promoters, while ES cells lacking the PRC2 component EED are deficient in Jarid2 promoter activity. Acts as a transcriptional repressor of ANF by binding to both GATA4 and NKX2-5 and repressing their transcriptional activator activities. Participates in the negative regulation of cell proliferation signaling. Interacts with the N-terminal region of GATA4 and the C- terminal region of the NKX2-5 homeobox and represses their ability to activate transcription of ANF.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6p24-p23

Cellular Component: ESC/E(Z) complex; histone methyltransferase complex; nucleoplasm; nucleus

Molecular Function: chromatin binding; histone demethylase activity

Biological Process: central nervous system development; chromatin modification; liver development; negative regulation of cell proliferation; negative regulation of gene expression, epigenetic; negative regulation of histone methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of histone H3-K9 methylation; spleen development; stem cell differentiation; thymus development; transcription, DNA-dependent