Rabbit Jak2 Polyclonal Antibody | anti-Jak2 antibody

Jak2 (Tyrosine-protein Kinase JAK2, Janus Kinase 2, JAK-2) (MaxLight 490)

Cat. Num. AAA6444694

• Gene Names: Jak2; Fd17
• Reactivity: Mouse, Human, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Jak2; Polyclonal Antibody; Jak2 (Tyrosine-protein Kinase JAK2; Janus Kinase 2; JAK-2) (MaxLight 490); anti-Jak2 antibody

• Host: Rabbit
• Reactivity: Mouse, Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the protein kinase 1 domain of mouse JAK2. Species Crossreactivity: human and rat.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
• Sequence Length: 1132

• Applicable Applications for anti-Jak2 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 0.5-1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Linear peptide corresponding to the protein kinase 1 domain of mouse JAK2, conjugated to KLH.
• Conjugate: MaxLight490
• Control: RAW264.7 cell lysate
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blotting Analysis: Representative lot data. RAW264.7 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-JAK2 (0.5ug/ml). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system. Arrow indicates JAK2 (~130kD).)

Western Blot (WB)

(Western Blotting Analysis: Representative lot data. L6 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-JAK2 (1ug/ml). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system. Arrow indicates JAK2 (~130kD).)

Product Categories/Family for anti-Jak2 antibody

Antibodies; Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 114326478
• NCBI GeneID: 16452
• NCBI Accession #: NP_001041642
• NCBI GenBank Nucleotide #: NP_001041642.1
• UniProt Accession #: Q62120
• Molecular Weight: 130
• NCBI Official Full Name: tyrosine-protein kinase JAK2
• NCBI Official Synonym Full Names: Janus kinase 2
• NCBI Official Symbol: Jak2
• NCBI Official Synonym Symbols: Fd17
• NCBI Protein Information: tyrosine-protein kinase JAK2
• UniProt Protein Name: Tyrosine-protein kinase JAK2
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Jak2
• UniProt Synonym Gene Names: JAK-2

Uniprot Description

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.