Rabbit JAK2 Polyclonal Antibody | anti-JAK2 antibody

JAK2, Phosphorylated (Y221) (Janus Kinase 2, JTK10, THCYT3) (APC)

Cat. Num. AAA6423351

• Gene Names: JAK2; JTK10; THCYT3
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: JAK2; Polyclonal Antibody; Phosphorylated (Y221) (Janus Kinase 2; JTK10; THCYT3) (APC); anti-JAK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes JAK2, phosphorylated Y221. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-JAK2 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding Y221 of human JAK2.
• Conjugate: APC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using phospho-JAK2-Y221 antibody.)

Product Categories/Family for anti-JAK2 antibody

Antibodies; Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4826776
• NCBI GeneID: 3717
• NCBI Accession #: NP_004963.1
• NCBI GenBank Nucleotide #: NM_004972.3
• UniProt Accession #: O60674; O14636; O75297
• Molecular Weight: 130,674 Da
• NCBI Official Full Name: tyrosine-protein kinase JAK2
• NCBI Official Synonym Full Names: Janus kinase 2
• NCBI Official Symbol: JAK2
• NCBI Official Synonym Symbols: JTK10; THCYT3
• NCBI Protein Information: tyrosine-protein kinase JAK2; JAK-2; Janus kinase 2 (a protein tyrosine kinase)
• UniProt Protein Name: Tyrosine-protein kinase JAK2
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: JAK2
• UniProt Synonym Gene Names: JAK-2
• UniProt Entry Name: JAK2_HUMAN

NCBI Description

This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]

Uniprot Description

JAK2: a non-receptor tyrosine-kinase involved in a specific subset of cytokine receptor signaling pathways, including IL-3, -5 and GM-CSF. Interacts with IL23R, SKB1 and STAM2. It has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. Fusion of Jak2 to TEL1 (ETV6) by t(9;12)(p24;p13) causes myeloproliferative disease in humans and mouse models. The Jak inhibitor AG490 inhibits constitutive Jak2 phosphorylation and causes apoptosis in cells from breast cancer and relapsing acute lymphoblastic leukemia. A single activating mutation is associated with several hematological malignancies. Inhibitor: AG490.

Protein type: Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Oncoprotein; TK group; JakA family

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: nucleoplasm; extrinsic to internal side of plasma membrane; cytoskeleton; nuclear matrix; cytoplasm; caveola; nucleus; cytosol; lipid raft

Molecular Function: protein C-terminus binding; histone binding; non-membrane spanning protein tyrosine kinase activity; acetylcholine receptor binding; protein kinase binding; protein kinase activity; interleukin-12 receptor binding; protein binding; growth hormone receptor binding; peptide hormone receptor binding; insulin receptor substrate binding; protein-tyrosine kinase activity; heme binding; phosphoinositide 3-kinase binding; type 1 angiotensin receptor binding; SH2 domain binding; ATP binding; receptor binding

Biological Process: establishment and/or maintenance of chromatin architecture; positive regulation of nitric oxide biosynthetic process; activation of MAPKK activity; tyrosine phosphorylation of Stat3 protein; response to lipopolysaccharide; tyrosine phosphorylation of JAK2 protein; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of apoptosis; response to antibiotic; elevation of cytosolic calcium ion concentration; tumor necrosis factor-mediated signaling pathway; erythrocyte differentiation; mesoderm development; negative regulation of neuron apoptosis; positive regulation of cell activation; positive regulation of DNA binding; positive regulation of protein import into nucleus, translocation; axon regeneration; positive regulation of insulin secretion; JAK-STAT cascade; positive regulation of tumor necrosis factor production; tyrosine phosphorylation of Stat5 protein; positive regulation of phosphoinositide 3-kinase cascade; tyrosine phosphorylation of STAT protein; positive regulation of peptidyl-tyrosine phosphorylation; response to hydroperoxide; mineralocorticoid receptor signaling pathway; tyrosine phosphorylation of Stat1 protein; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of cell differentiation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; hormone-mediated signaling; negative regulation of heart contraction; apoptosis; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; signal transduction; host programmed cell death induced by symbiont; negative regulation of cell proliferation; actin filament polymerization; positive regulation of cell proliferation; cell differentiation; STAT protein nuclear translocation; caspase activation; cell migration; cytokine and chemokine mediated signaling pathway; negative regulation of DNA binding; regulation of cell proliferation; positive regulation of interleukin-1 beta production; G-protein coupled receptor protein signaling pathway; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of inflammatory response; innate immune response; negative regulation of cell-cell adhesion; cell motility; blood coagulation; induction of apoptosis by oxidative stress; positive regulation of cell migration; positive regulation of inflammatory response

Disease: Polycythemia Vera; Myelofibrosis; Budd-chiari Syndrome; Erythrocytosis, Familial, 1; Thrombocythemia 3