Human S100 Calcium Binding Protein A4 (S100A4) ELISA Kit | S100A4 elisa kit

Human S100 Calcium Binding Protein A4 (S100A4) High Sensitive ELISA Kit

Cat. Num. AAA2087065

• Gene Names: S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
• Reactivity: Human
• Synonyms: S100 Calcium Binding Protein A4 (S100A4); Human S100 Calcium Binding Protein A4 (S100A4) High Sensitive ELISA Kit; S100-A4; MTS1; 18A2; 42A; CAPL; P9KA; PEL98; Calcium protein; Calvasculin; Metastasin; Protein Mts1; Placental calcium-binding protein; Murine placental homolog; S100A4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive S100 Calcium Binding Protein A4 (S100A4).; No significant cross-reactivity or interference between High Sensitive S100 Calcium Binding Protein A4 (S100A4) and analogues was observed.
• Sequence Length: 101

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 78-5000pg/mL
• Sensitivity: < 30pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive S100 Calcium Binding Protein A4 (S100A4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive S100 Calcium Binding Protein A4 (S100A4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive S100 Calcium Binding Protein A4 (S100A4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive S100 Calcium Binding Protein A4 (S100A4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive S100 Calcium Binding Protein A4 (S100A4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive S100 Calcium Binding Protein A4 (S100A4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for S100A4 elisa kit

Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 4506765
• NCBI GeneID: 6275
• NCBI Accession #: NP_002952.1
• NCBI GenBank Nucleotide #: NM_002961.2
• UniProt Accession #: P26447; Q6ICP8; A8K7R8; D3DV46
• Molecular Weight: 11,729 Da
• NCBI Official Full Name: protein S100-A4
• NCBI Official Synonym Full Names: S100 calcium binding protein A4
• NCBI Official Symbol: S100A4
• NCBI Official Synonym Symbols: 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
• NCBI Protein Information: protein S100-A4
• UniProt Protein Name: Protein S100-A4
• Protein Family: Protein
• UniProt Gene Name: S100A4
• UniProt Synonym Gene Names: CAPL; MTS1

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A4: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Protein type: Calcium-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: extracellular space; nucleus; perinuclear region of cytoplasm

Molecular Function: protein binding; RAGE receptor binding

Biological Process: epithelial to mesenchymal transition; positive regulation of I-kappaB kinase/NF-kappaB cascade