Western Blot (WB)
(Western blot using MBS611894 shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134kD in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains ~ 20ug of lysate. MBS611894 was used at a 1:250 dilution. The membrane was washed and reacted with IgG (HRP) Pab Gt xRb Exposure time was 1 min. Predicted molecular weight is 134kD.)
Rabbit anti-Human, Mouse Jagged1 Polyclonal Antibody | anti-JAG1 antibody
Jagged1 (JAG1, AGS, AHD, AWS, HJ1, JAGL1, Alagille syndrome protein)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western blot using MBS611894 shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134kD in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains ~ 20ug of lysate. MBS611894 was used at a 1:250 dilution. The membrane was washed and reacted with IgG (HRP) Pab Gt xRb Exposure time was 1 min. Predicted molecular weight is 134kD.)
Western Blot (WB)
(Western blot using MBS611894 shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134kD in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains ~ 20ug of lysate. MBS611894 was used at a 1:250 dilution. The membrane was washed and reacted with IgG (HRP) Pab Gt xRb Exposure time was 1 min. Predicted molecular weight is 134kD.)
Immunofluorescence (IF)
(Immunofluorescence Microscopy using MBS611894 on human corneal epithelial cells. MBS611894 was used at a 1:250 dilution. The Jagged1 (green staining) is local-ized to the cytoplasm and is consistentwith reports in the literature. The nucleus is stained with Bis benzimide (blue).)
Immunofluorescence (IF)
(Immunofluorescence Microscopy using MBS611894 on human corneal epithelial cells. MBS611894 was used at a 1:250 dilution. The Jagged1 (green staining) is local-ized to the cytoplasm and is consistentwith reports in the literature. The nucleus is stained with Bis benzimide (blue).)
Immunohistochemistry (IHC)
(Immunohistochemical staining of human cervical cancer tissue (40X magnification) using MBS611894. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:50 dilution of MBS611894 was used.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of human cervical cancer tissue (40X magnification) using MBS611894. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:50 dilution of MBS611894 was used.)
NCBI and Uniprot Product Information
NCBI Description
The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq]
Uniprot Description
JAG1: Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Interacts with NOTCH2 and NOTCH3. Interacts with NOTCH1 (in the presence of calcium ions). Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 20p12.1-p11.23
Cellular Component: membrane; apical part of cell; integral to plasma membrane; extracellular region; plasma membrane
Molecular Function: protein binding; growth factor activity; calcium ion binding; Notch binding; structural molecule activity
Biological Process: nervous system development; endothelial cell differentiation; Notch signaling pathway; positive regulation of myeloid cell differentiation; T cell mediated immunity; Notch receptor processing; auditory receptor cell differentiation; cell fate determination; negative regulation of fat cell differentiation; regulation of cell migration; regulation of cell proliferation; myoblast differentiation; keratinocyte differentiation; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; response to muramyl dipeptide; morphogenesis of an epithelial sheet; positive regulation of transcription from RNA polymerase II promoter; hemopoiesis; blood vessel remodeling; angiogenesis; positive regulation of Notch signaling pathway
Disease: Tetralogy Of Fallot; Alagille Syndrome 1
Research Articles on JAG1
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Product Notes
The JAG1 (Catalog #AAA611894) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Jagged1 (JAG1, AGS, AHD, AWS, HJ1, JAGL1, Alagille syndrome protein) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Jagged1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunofluorescence. Researchers should empirically determine the suitability of the JAG1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Jagged1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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