Rabbit anti-Human IVD Polyclonal Antibody | anti-IVD antibody

Anti-IVD Antibody, Rabbit Polyclonal

Cat. Num. AAA8107257

• Gene Names: IVD; ACAD2
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: IVD; Polyclonal Antibody; Anti-IVD Antibody; Rabbit Polyclonal; Isovaleryl-CoA dehydrogenase/IVD Antibody; Rabbit PAb; Antigen Affinity Purified; isovaleryl-CoA dehydrogenase; Anti-ACAD2 Antibody; anti-IVD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Isovaleryl-CoA dehydrogenase/IVD
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IVD antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Human Isovaleryl-CoA dehydrogenase/IVD Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Isovaleryl-CoA dehydrogenase/IVD (rh Isovaleryl-CoA dehydrogenase/IVD). Isovaleryl-CoA dehydrogenase/IVD specific IgG was purified by Human Isovaleryl-CoA dehydrogenase/IVD affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-IVD rabbit polyclonal antibody at 1:500 dilution Lane A: IVD konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:46 kDa Observed band size:44 kDa)

Immunoprecipitation (IP)

(IVD was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-IVD rabbit polyclonal antibody and 15 ul of  50 %  Protein G agarose.Primary antibody:Anti-IVD rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa)

Western Blot (WB)

(Anti-IVDï(30-423) rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat  Whole Cell LysateLane C: Hela  Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:46 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-IVD antibody

IVD (Isovaleryl-CoA Dehydrogenase) is a Protein Coding gene. IVD is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. IVD plays an essential role in processing proteins obtained from the diet. The body breaks down proteins from food into smaller parts called amino acids. Amino acids can be further processed to provide energy for growth and development. Isovaleryl-CoA dehydrogenase helps process a particular amino acid called leucine. Specifically, isovaleryl-CoA dehydrogenase is responsible for the third step in the breakdown of leucine. This step is a chemical reaction that converts a molecule called isovaleryl-CoA to another molecule, 3-methylcrotonyl-CoA. Additional chemical reactions convert 3-methylcrotonyl-CoA into molecules that are used for energy.

References

BACHHAWAT BK, et al. (1956) Enzymatic carboxylation of beta-hydroxyisovaleryl coenzyme A. J Biol Chem. 219(2):539-50. Ikeda Y, et al. (1983) Purification and characterization of isovaleryl coenzyme A dehydrogenase from rat liver mitochondria. J Biol Chem. 258(2):1077-85. Tanaka K, et al. (1966) Enzymatic carboxylation of beta-hydroxyisovaleryl coenzyme A. J Biol Chem. 219(2):539-50.

NCBI and Uniprot Product Information

• NCBI GI #: 16877964
• NCBI GeneID: 3712
• Molecular Weight: 42,724 Da
• NCBI Official Full Name: Isovaleryl Coenzyme A dehydrogenase
• NCBI Official Synonym Full Names: isovaleryl-CoA dehydrogenase
• NCBI Official Symbol: IVD
• NCBI Official Synonym Symbols: ACAD2
• NCBI Protein Information: isovaleryl-CoA dehydrogenase, mitochondrial; isovaleryl CoA dehydrogenase; isovaleryl Coenzyme A dehydrogenase
• Protein Family: Isovaleryl-CoA dehydrogenase

NCBI Description

Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]