Rabbit anti-Human ITPK1 Polyclonal Antibody | anti-ITPK1 antibody

ITPK1, NT (ITPK1, Inositol-tetrakisphosphate 1-kinase, Inositol 1,3,4-trisphosphate 5/6-kinase) (MaxLight 490)

Cat. Num. AAA6311417

• Gene Names: ITPK1; ITRPK1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ITPK1; Polyclonal Antibody; NT (ITPK1; Inositol-tetrakisphosphate 1-kinase; Inositol 1; 3; 4-trisphosphate 5/6-kinase) (MaxLight 490); 4-trisphosphate 5/6-kinase; anti-ITPK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-ITPK1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ITPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-54 amino acids from the N-terminal region of human ITPK1.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-ITPK1 antibody

Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetraphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain.

Product Categories/Family for anti-ITPK1 antibody

Antibodies; Protein Kinases

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010). Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010). Yoshida, T., et al. Int. J. Mol. Med. 24(4):539-547(2009). Chamberlain, P.P., et al. J. Biol. Chem. 282(38):28117-28125(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 217272844
• NCBI GeneID: 3705
• NCBI Accession #: NP_001136065.1
• NCBI GenBank Nucleotide #: NM_001142593.1
• UniProt Accession #: Q13572; Q9BTL6; Q9H2E7
• Molecular Weight: 45,621 Da
• NCBI Official Full Name: inositol-tetrakisphosphate 1-kinase isoform a
• NCBI Official Synonym Full Names: inositol-tetrakisphosphate 1-kinase
• NCBI Official Symbol: ITPK1
• NCBI Official Synonym Symbols: ITRPK1
• NCBI Protein Information: inositol-tetrakisphosphate 1-kinase; ins(1,3,4)P(3) 5/6-kinase; inositol-triphosphate 5/6-kinase; inositol 1,3,4-triphosphate 5/6 kinase; inositol 1,3,4-trisphosphate 5/6 kinase; inositol 1,3,4-trisphosphate 5/6-kinase
• UniProt Protein Name: Inositol-tetrakisphosphate 1-kinase
• Protein Family: Inositol-tetrakisphosphate 1-kinase
• UniProt Gene Name: ITPK1
• UniProt Synonym Gene Names: Inositol-triphosphate 5/6-kinase
• UniProt Entry Name: ITPK1_HUMAN

Uniprot Description

ITPK1: Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain. Belongs to the ITPK1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, lipid; EC 2.7.1.134; EC 2.7.1.159; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 14q31

Cellular Component: cytosol

Molecular Function: magnesium ion binding; inositol tetrakisphosphate 1-kinase activity; isomerase activity; catalytic activity; ATP binding

Biological Process: inositol phosphate metabolic process; dephosphorylation; signal transduction; blood coagulation; phosphorylation; inositol trisphosphate metabolic process