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Western Blot (WB) (Western Blot: Sample: Recombinant ALB, Human.)

Rabbit anti-Human Albumin (ALB) Polyclonal Antibody | anti-ALB antibody

Polyclonal Antibody to Albumin (ALB)

Gene Names
ALB; HSA; PRO0883; PRO0903; PRO1341
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Albumin (ALB); Polyclonal Antibody; Polyclonal Antibody to Albumin (ALB); Serum albumin; anti-ALB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ALB. It has been selected for its ability to recognize ALB in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
609
Applicable Applications for anti-ALB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Immunogen
Eukaryotic Albumin (ALB)
Cross Reactivity
Human
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant ALB, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant ALB, Human.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
213
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,160 Da
NCBI Official Full Name
serum albumin preproprotein
NCBI Official Synonym Full Names
albumin
NCBI Official Symbol
ALB
NCBI Official Synonym Symbols
HSA; PRO0883; PRO0903; PRO1341
NCBI Protein Information
serum albumin
UniProt Protein Name
Serum albumin
Protein Family
UniProt Gene Name
ALB

NCBI Description

This gene encodes the most abundant protein in human blood. This protein functions in the regulation of blood plasma colloid osmotic pressure and acts as a carrier protein for a wide range of endogenous molecules including hormones, fatty acids, and metabolites, as well as exogenous drugs. Additionally, this protein exhibits an esterase-like activity with broad substrate specificity. The encoded preproprotein is proteolytically processed to generate the mature protein. A peptide derived from this protein, EPI-X4, is an endogenous inhibitor of the CXCR4 chemokine receptor. [provided by RefSeq, Jul 2016]

Uniprot Description

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carrier; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: blood microparticle; endoplasmic reticulum; endoplasmic reticulum lumen; extracellular exosome; extracellular region; extracellular space; Golgi apparatus; myelin sheath; nucleus; protein complex

Molecular Function: antioxidant activity; chaperone binding; copper ion binding; DNA binding; drug binding; fatty acid binding; identical protein binding; oxygen binding; protein binding; pyridoxal phosphate binding; toxin binding

Biological Process: bile acid and bile salt transport; cellular oxidant detoxification; cellular protein metabolic process; cellular response to starvation; hemolysis by symbiont of host red blood cells; high-density lipoprotein particle remodeling; maintenance of mitochondrion location; negative regulation of apoptosis; negative regulation of programmed cell death; platelet degranulation; post-translational protein modification; receptor-mediated endocytosis; retinal homeostasis; sodium-independent organic anion transport

Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic

Research Articles on ALB

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Product Notes

The ALB alb (Catalog #AAA2090526) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Albumin (ALB) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Albumin (ALB) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Researchers should empirically determine the suitability of the ALB alb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Albumin (ALB), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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