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Western Blot (WB) (Western blot analysis of ITM2A Antibody (N-term) in K562 cell line lysates (35ug/lane). ITM2A (arrow) was detected using the purified Pab.)

Rabbit anti-Human ITM2A Polyclonal Antibody | anti-ITM2A antibody

ITM2A Antibody (N-term)

Gene Names
ITM2A; E25A; BRICD2A
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ITM2A; Polyclonal Antibody; ITM2A Antibody (N-term); Integral membrane protein 2A; Protein E25; anti-ITM2A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ITM2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-49 amino acids from the N-terminal region of human ITM2A.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
22-49
Sequence Length
219
Applicable Applications for anti-ITM2A antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of ITM2A Antibody (N-term) in K562 cell line lysates (35ug/lane). ITM2A (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ITM2A Antibody (N-term) in K562 cell line lysates (35ug/lane). ITM2A (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ITM2A (arrow) using rabbit polyclonal ITM2A Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ITM2A gene (Lane 2).)

Western Blot (WB) (Western blot analysis of ITM2A (arrow) using rabbit polyclonal ITM2A Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ITM2A gene (Lane 2).)

Flow Cytometry (FC/FACS)

(ITM2A Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ITM2A Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ITM2A antibody
ITM2A is induced during thymocyte selection and T cell activation and causes downregulation of CD8 when overexpressed in CD4(+)CD8(+) double positive thymocytes.
Product Categories/Family for anti-ITM2A antibody
References
Gudbjartsson,D.F., et.al., Nat. Genet. 40 (5), 609-615 (2008)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29741
NCBI Official Full Name
integral membrane protein 2A isoform 2
NCBI Official Synonym Full Names
integral membrane protein 2A
NCBI Official Symbol
ITM2A
NCBI Official Synonym Symbols
E25A; BRICD2A
NCBI Protein Information
integral membrane protein 2A
UniProt Protein Name
Integral membrane protein 2A
Protein Family
UniProt Gene Name
ITM2A
UniProt Entry Name
ITM2A_HUMAN

NCBI Description

This gene encodes a type II membrane protein that belongs to the ITM2 family. Studies in mouse suggest that it may be involved in osteo- and chondrogenic differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

ITM2A: a type II membrane protein that belongs to the ITM2 family. Studies in mouse suggest that it may be involved in osteo- and chondrogenic differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq13.3-Xq21.2

Cellular Component: integral to membrane

Molecular Function: protein binding

Research Articles on ITM2A

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Product Notes

The ITM2A itm2a (Catalog #AAA9202981) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 22-49. The ITM2A Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ITM2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the ITM2A itm2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITM2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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