Western Blot (WB)
(Western blot analysis of ITIH2 antibody (C-term) in HepG2 cell line lysates (35ug/lane). ITIH2 (arrow) was detected using the purified Pab.)
Rabbit anti-Human ITIH2 Polyclonal Antibody | anti-ITIH2 antibody
ITIH2, CT (Inter-alpha-Trypsin Inhibitor Heavy Chain H2, Inter-alpha-Inhibitor Heavy Chain 2, ITI Heavy Chain H2, ITI-HC2, H2P, IGHEP2, Inter-alpha-Trypsin Inhibitor Complex Component II, Serum-derived Hyaluronan-associated Protein, SHAP) (FITC)
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
ITIH2; Polyclonal Antibody; CT (Inter-alpha-Trypsin Inhibitor Heavy Chain H2; Inter-alpha-Inhibitor Heavy Chain 2; ITI Heavy Chain H2; ITI-HC2; H2P; IGHEP2; Inter-alpha-Trypsin Inhibitor Complex Component II; Serum-derived Hyaluronan-associated Protein; SHAP) (FITC); Inter-alpha (Globulin) Inhibitor H2; anti-ITIH2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ITIH2.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Sequence Length
946
Applicable Applications for anti-ITIH2 antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA)
Application Notes
IHC: 1:50-1:100
FC/FACS: 1:10-1:50
WB: 1:50-1:100
Applications are based on unconjugated antibody.
FC/FACS: 1:10-1:50
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to a portion of amino acids within aa865-894 from the C-terminal region of human ITIH2, conjugated to KLH. Cellular Localization: Secreted
Conjugate
FITC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of ITIH2 antibody (C-term) in HepG2 cell line lysates (35ug/lane). ITIH2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ITIH2 antibody (C-term) in HepG2 cell line lysates (35ug/lane). ITIH2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ITIH2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ITIH2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ITIH2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ITIH2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-ITIH2 antibody
References
1. Obayashi, Y., Oncol. Rep. 19(5), 1245-1251 (2008). 2. Kishida, T., Connect. Tissue Res. 49(2), 105-108 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
106.463
NCBI Official Full Name
inter-alpha-trypsin inhibitor heavy chain H2
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H2
Protein Family
UniProt Gene Name
ITIH2
UniProt Synonym Gene Names
IGHEP2; ITI heavy chain H2; ITI-HC2; Inter-alpha-inhibitor heavy chain 2; SHAP
UniProt Entry Name
ITIH2_HUMAN
Uniprot Description
ITIH2: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family.
Protein type: Secreted, signal peptide; Inhibitor; Secreted
Chromosomal Location of Human Ortholog: 10p15
Cellular Component: extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity
Biological Process: hyaluronan metabolic process
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Product Notes
The ITIH2 itih2 (Catalog #AAA6483670) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ITIH2, CT (Inter-alpha-Trypsin Inhibitor Heavy Chain H2, Inter-alpha-Inhibitor Heavy Chain 2, ITI Heavy Chain H2, ITI-HC2, H2P, IGHEP2, Inter-alpha-Trypsin Inhibitor Complex Component II, Serum-derived Hyaluronan-associated Protein, SHAP) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ITIH2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA). IHC: 1:50-1:100 FC/FACS: 1:10-1:50 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ITIH2 itih2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITIH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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