Rabbit ITGB1 Polyclonal Antibody | anti-ITGB1 antibody

ITGB1 Antibody (C-term)

Cat. Num. AAA9203852

• Gene Names: ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
• Reactivity: Human (Predicted Reactivity: Xenopus, Bovine, Chicken, Mouse, Pig, Rat, Sheep)
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ITGB1; Polyclonal Antibody; ITGB1 Antibody (C-term); Integrin beta-1; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta; CD29; FNRB; MDF2; MSK12; anti-ITGB1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Xenopus, Bovine, Chicken, Mouse, Pig, Rat, Sheep)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ITGB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 759-785 amino acids from the C-terminal region of human ITGB1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 759-785
• Sequence Length: 801

• Applicable Applications for anti-ITGB1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Function: Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G- E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha- 4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha- 11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha- 4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha- 3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha- 3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G- D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430). ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed:31331973).
• Cellular Location: Cell membrane; Single-pass type I membrane protein. Cell projection, invadopodium membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Recycling endosome Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Cell junction, focal adhesion. Cell surface. Note=Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid- telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner Localized at plasma and ruffle membranes in a collagen-independent manner.
• Tissue Location: [Isoform 1]: Widely expressed, other isoforms are generally coexpressed with a more restricted distribution [Isoform 3]: Together with isoform 4, is expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. [Isoform 5]: Expressed specifically in striated muscle (skeletal and cardiac muscle).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(ITGB1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the ITGB1 antibody detected the ITGB1 protein (arrow).)

Related Product Information for anti-ITGB1 antibody

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.

Product Categories/Family for anti-ITGB1 antibody

Cancer; Cardiovascular; Developmental Biology; Immunology; Neuroscience; Signal Transduction

References

Martchenko, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(35):15583-15588(2010) Caicedo-Carvajal, C.E., et al. PLoS ONE 5 (7), E11830 (2010) : Rikova, K., et al. Cell 131(6):1190-1203(2007) Seales, E.C., et al. J. Biol. Chem. 280(45):37610-37615(2005) Armulik, A. Front. Biosci. 7, D219-D227 (2002) :

NCBI and Uniprot Product Information

• NCBI GI #: 19743813
• NCBI GeneID: 3688
• NCBI Accession #: NP_002202.2; NP_391988.1; NP_596867.1
• NCBI GenBank Nucleotide #: NM_002211.3
• UniProt Accession #: P05556; P78466; P78467; Q13089; Q13090; Q13091; Q13212; A8K6N2; D3DRX9; D3DRY3; D3DRY4; D3DRY5
• NCBI Official Full Name: integrin beta-1 isoform 1A
• NCBI Official Synonym Full Names: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• NCBI Official Symbol: ITGB1
• NCBI Official Synonym Symbols: CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
• NCBI Protein Information: integrin beta-1
• UniProt Protein Name: Integrin beta-1
• Protein Family: Integrin
• UniProt Gene Name: ITGB1
• UniProt Synonym Gene Names: FNRB; MDF2; MSK12; GPIIA
• UniProt Entry Name: ITB1_HUMAN

NCBI Description

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral; Cell surface; Cell adhesion

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: cell surface; focal adhesion; dendritic spine; acrosome; lipid raft; ruffle; recycling endosome; membrane; hemidesmosome; lamellipodium; cytoplasm; plasma membrane; melanosome; basement membrane; neuromuscular junction; integrin complex; receptor complex; sarcolemma; filopodium; external side of plasma membrane; cleavage furrow

Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; actin binding; alpha-actinin binding; peptide binding; protein kinase binding; viral receptor activity; integrin binding; protein binding; protein heterodimerization activity; fibronectin binding; cell adhesion molecule binding; glycoprotein binding

Biological Process: axon guidance; entry of virus into host cell; extracellular matrix organization and biogenesis; maternal process involved in pregnancy; positive regulation of apoptosis; regulation of cell cycle; axon extension; cell-matrix adhesion; mesodermal cell differentiation; positive regulation of endocytosis; cell fate specification; cardiac muscle cell differentiation; negative regulation of cell proliferation; leukocyte adhesion; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; germ cell migration; tissue homeostasis; visual learning; negative regulation of cell projection organization and biogenesis; integrin-mediated signaling pathway; response to drug; cell migration; regulation of immune response; in utero embryonic development; dendrite morphogenesis; sarcomere organization; protein transport within lipid bilayer; cell-substrate adhesion; cellular calcium ion homeostasis; formation of radial glial scaffolds; heterotypic cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of neuron differentiation; cell migration during sprouting angiogenesis; negative regulation of Rho protein signal transduction; B cell differentiation; cell-cell adhesion mediated by integrin; stress fiber formation; calcium-independent cell-matrix adhesion; cellular defense response; response to activity; blood coagulation; homophilic cell adhesion; leukocyte tethering or rolling; leukocyte migration; regulation of G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; positive regulation of cell migration