Rabbit anti-Human ITGA8 Polyclonal Antibody | anti-ITGA8 antibody

ITGA8, CT (ITGA8, Integrin alpha-8, Integrin alpha-8 heavy chain, Integrin alpha-8 light chain) (MaxLight 750)

Cat. Num. AAA6311211

• Gene Names: ITGA8; ITGA8
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ITGA8; Polyclonal Antibody; CT (ITGA8; Integrin alpha-8; Integrin alpha-8 heavy chain; Integrin alpha-8 light chain) (MaxLight 750); Integrin alpha-8 light chain; anti-ITGA8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ITGA8.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-ITGA8 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1025-1053 in the C-terminal region of human ITGA8.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6009536. This demonstrates that MBS6009536 detected the ITGA8 protein (arrow).)

Western Blot (WB)

(Western Blot using MBS6009536 (1:2000) Lane 1: Raji whole cell lysate Lane 2: 293 whole cell lysate Lane 3: LNCaP whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit at 1:10,000. Predicted band size: 117kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS6009536 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6009536 for immunohistochemistry.)

Product Categories/Family for anti-ITGA8 antibody

Antibodies; Integrin

References

1. Benoit, Y.D., et al. Biochem. Biophys. Res. Commun. 399(3):434-439(2010). 2. Simon-Sanchez, J., et al. Nat. Genet. 41(12):1308-1312(2009). 3. Benoit, Y.D., et al. Biol. Cell 101(12):695-708(2009). 4. Sato, Y., et al. J. Biol. Chem. 284(21):14524-14536(2009). 5. Lowe, J.K., et al. PLoS Genet. 5 (2), E1000365 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 49170034
• NCBI GeneID: 8516
• NCBI Accession #: NP_003629.1
• NCBI GenBank Nucleotide #: NM_003638.1
• UniProt Accession #: P53708; Q5VX94; B0YJ31
• Molecular Weight: 117,474 Da
• NCBI Official Full Name: integrin alpha-8
• NCBI Official Synonym Full Names: integrin, alpha 8
• NCBI Official Symbol: ITGA8
• NCBI Official Synonym Symbols: ITGA8
• NCBI Protein Information: integrin alpha-8; OTTHUMP00000019207
• UniProt Protein Name: Integrin alpha-8
• Protein Family: Integrin
• UniProt Gene Name: ITGA8
• UniProt Entry Name: ITA8_HUMAN

NCBI Description

Integrins are heterodimeric transmembrane receptor proteins that mediate numerous cellular processes including cell adhesion, cytoskeletal rearrangement, and activation of cell signaling pathways. Integrins are composed of alpha and beta subunits. This gene encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1 protein. The encoded protein is a single-pass type 1 membrane protein that contains multiple FG-GAP repeats. This repeat is predicted to fold into a beta propeller structure. This gene regulates the recruitment of mesenchymal cells into epithelial structures, mediates cell-cell interactions, and regulates neurite outgrowth of sensory and motor neurons. The integrin alpha8beta1 protein thus plays an important role in wound-healing and organogenesis. Mutations in this gene have been associated with renal hypodysplasia/aplasia-1 (RHDA1) and with several animal models of chronic kidney disease. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2014]

Uniprot Description

ITGA8: Integrin alpha-8/beta-1 functions in the genesis of kidney and probably of other organs by regulating the recruitment of mesenchymal cells into epithelial structures. It recognizes the sequence R-G-D in a wide array of ligands including TNC, FN1, SPP1 TGFB1, TGFB3 and VTN. NPNT is probably its functional ligand in kidney genesis. Neuronal receptor for TNC it mediates cell-cell interactions and regulates neurite outgrowth of sensory and motor neurons. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: focal adhesion; cell surface; endoplasmic reticulum; apical part of cell; postsynaptic density; plasma membrane; perikaryon; integrin complex

Molecular Function: metal ion binding

Biological Process: integrin-mediated signaling pathway; smooth muscle development; establishment of protein localization; cell-cell adhesion; extracellular matrix organization and biogenesis; inner ear morphogenesis; cell projection organization and biogenesis; cell-matrix adhesion; positive regulation of transforming growth factor beta receptor signaling pathway; mesodermal cell differentiation; brain development; kidney development; metanephros development; memory

Disease: Renal Hypodysplasia/aplasia 1