Integrin alpha 8 beta 1 Recombinant Protein | ITGA8&ITGB1 recombinant protein

Integrin alpha 8 beta 1 Protein, Human, Recombinant (DYKDDDDK & His Tag)

Cat. Num. AAA8123607

• Gene Names: ITGA8; ITGA8
• Purity: >85% as determined by SDS-PAGE
• Synonyms: Integrin alpha 8 beta 1; Integrin alpha 8 beta 1 Protein; Human; Recombinant (DYKDDDDK & His Tag); Human ITGA8 & ITGB1 Heterodimer Protein; integrin; alpha 8; ITGA8&ITGB1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu1012&Met1-Asp728

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Phe 39 & Gln 21
• Tag: C-DYKDDDDK & C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Leu 1012) of human ITGA8 (P53708) was fused with a DYKDDDDK tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 728) of human ITGB1 (P05556-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Product Categories/Family for ITGA8&ITGB1 recombinant protein

AKT Signaling Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 49170034
• NCBI GeneID: 8516
• NCBI Accession #: NP_003629.1
• NCBI GenBank Nucleotide #: NM_003638.1
• UniProt Accession #: P53708; P05556
• Molecular Weight: 117,474 Da
• NCBI Official Full Name: integrin alpha-8
• NCBI Official Synonym Full Names: integrin, alpha 8
• NCBI Official Symbol: ITGA8
• NCBI Official Synonym Symbols: ITGA8
• NCBI Protein Information: integrin alpha-8; OTTHUMP00000019207
• UniProt Protein Name: Integrin alpha-8
• UniProt Gene Name: ITGA8
• UniProt Entry Name: ITA8_HUMAN

NCBI Description

Integrins are heterodimeric transmembrane receptor proteins that mediate numerous cellular processes including cell adhesion, cytoskeletal rearrangement, and activation of cell signaling pathways. Integrins are composed of alpha and beta subunits. This gene encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1 protein. The encoded protein is a single-pass type 1 membrane protein that contains multiple FG-GAP repeats. This repeat is predicted to fold into a beta propeller structure. This gene regulates the recruitment of mesenchymal cells into epithelial structures, mediates cell-cell interactions, and regulates neurite outgrowth of sensory and motor neurons. The integrin alpha8beta1 protein thus plays an important role in wound-healing and organogenesis. Mutations in this gene have been associated with renal hypodysplasia/aplasia-1 (RHDA1) and with several animal models of chronic kidney disease. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2014]

Uniprot Description

ITGA8: Integrin alpha-8/beta-1 functions in the genesis of kidney and probably of other organs by regulating the recruitment of mesenchymal cells into epithelial structures. It recognizes the sequence R-G-D in a wide array of ligands including TNC, FN1, SPP1 TGFB1, TGFB3 and VTN. NPNT is probably its functional ligand in kidney genesis. Neuronal receptor for TNC it mediates cell-cell interactions and regulates neurite outgrowth of sensory and motor neurons. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: focal adhesion; cell surface; endoplasmic reticulum; apical part of cell; postsynaptic density; plasma membrane; perikaryon; integrin complex

Molecular Function: metal ion binding

Biological Process: integrin-mediated signaling pathway; smooth muscle development; establishment of protein localization; cell-cell adhesion; extracellular matrix organization and biogenesis; inner ear morphogenesis; cell projection organization and biogenesis; cell-matrix adhesion; positive regulation of transforming growth factor beta receptor signaling pathway; mesodermal cell differentiation; brain development; kidney development; metanephros development; memory

Disease: Renal Hypodysplasia/aplasia 1