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Immunofluorescence (IF)
(Confocal immunofluorescent analysis of IDH1 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human, Mouse Isocitrate Dehydrogenase [NADP], Cytoplasmic Polyclonal Antibody | anti-ICDH antibody
Isocitrate Dehydrogenase [NADP], Cytoplasmic (IDH, IDCD, IDH1, IDP, IDPC, Cytosolic NADP-isocitrate Dehydrogenase, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase, PICD)
Gene Names
ICDH; F20D21.16; F20D21_16; ICDH; isocitrate dehydrogenase
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
Isocitrate Dehydrogenase [NADP]; Cytoplasmic; Polyclonal Antibody; Cytoplasmic (IDH; IDCD; IDH1; IDP; IDPC; Cytosolic NADP-isocitrate Dehydrogenase; NADP(+)-specific ICDH; Oxalosuccinate Decarboxylase; PICD); Anti -Isocitrate Dehydrogenase [NADP]; anti-ICDH antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human IDH1 at ~47kD. Species Crossreactivity: mouse.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-ICDH antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1000
Western Blot: 1:50-1:100
Dilution: ELISA: 1:1000
Western Blot: 1:50-1:100
Immunogen
KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa122-152 from the center region of human IDH1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of IDH1 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of IDH1 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB)
(Western blot analysis of IDH1 Antibody (Center) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). IDH1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of IDH1 Antibody (Center) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). IDH1 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(IDH1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(IDH1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ICDH antibody
IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Product Categories/Family for anti-ICDH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,234 Da
NCBI Official Full Name
isocitrate dehydrogenase
NCBI Official Symbol
ICDH
NCBI Official Synonym Symbols
F20D21.16; F20D21_16; ICDH; isocitrate dehydrogenase
NCBI Protein Information
isocitrate dehydrogenase
UniProt Protein Name
Isocitrate dehydrogenase [NADP]
Protein Family
UniProt Gene Name
F20D21.16
UniProt Synonym Gene Names
ICDH
UniProt Entry Name
Q9SLK0_ARATH
NCBI Description
NADP-specific isocitrate dehydrogenase (ICDH)
Uniprot Description
Catalytic activity: Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108Oxalosuccinate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108
Cofactor: Binds 1 magnesium or manganese ion per subunit
By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3
Sequence similarities: Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108
Research Articles on ICDH
Similar Products
Product Notes
The ICDH f20d21.16 (Catalog #AAA629500) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Isocitrate Dehydrogenase [NADP], Cytoplasmic (IDH, IDCD, IDH1, IDP, IDPC, Cytosolic NADP-isocitrate Dehydrogenase, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase, PICD) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Isocitrate Dehydrogenase [NADP], Cytoplasmic can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the ICDH f20d21.16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Isocitrate Dehydrogenase [NADP], Cytoplasmic, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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