Phosphodiesterase 1A Recombinant Protein | PDE1A recombinant protein

Recombinant Phosphodiesterase 1A, Calmodulin Dependent (PDE1A)

Cat. Num. AAA2029118

• Gene Names: Pde1a; 3-RACEclone8phosphodiesterase1A; 3'-RACEclone8phosphodiesterase1A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Phosphodiesterase 1A; Recombinant Phosphodiesterase 1A; Calmodulin Dependent (PDE1A); PDE1A recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; GSSATEIEE LENTTFKYLT GEQTEKMWQR LKGILRCLVK QLERGDVNVVDLKKNIEYAA SVLEAVYIDE TRRLLDTEDE LSDIQTDSVP SEVRDWLASTFTRKMGMTKK KPEEKPKFRS IVHAVQAGIF VERMYRKTYH MVGLAYPAAVIVTLKDVDKW SFDVFALNEA SGEHSLKFMI YELFTRYDLI NRFKIPVSCLITFAEALEVG YSKYKNPYHN LIHAADVTQT VHYIMLHTGI MHWLTELEILAMVFAAAIHD YEHTGTTNNF HIQTRSDVAI LYNDRSVLEN HHVSAAYRLMQEEEMNILIN LSKDDWRD
• Sequence Length: 542

• Applicable Applications for PDE1A recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 40.4kDa
• Accurate Molecular Mass (KD): 40kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Gly2~Asp318
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 12083139
• NCBI GeneID: 81529
• Molecular Weight: 61,863 Da
• NCBI Official Full Name: phosphodiesterase 1A
• NCBI Official Synonym Full Names: phosphodiesterase 1A, calmodulin-dependent
• NCBI Official Symbol: Pde1a
• NCBI Official Synonym Symbols: 3-RACEclone8phosphodiesterase1A; 3'-RACEclone8phosphodiesterase1A
• NCBI Protein Information: calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
• UniProt Protein Name: Phosphodiesterase 1A
• Protein Family: Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase
• UniProt Gene Name: Pde1a
• UniProt Entry Name: Q9EPR9_RAT

NCBI Description

expression in the hippocampus increases in response to electroconvulsive shock [RGD, Feb 2006]

Uniprot Description

PDE1A: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.17; Phosphodiesterase; Nucleotide Metabolism - purine

Cellular Component: cell soma; cytoplasm; nucleus

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity; metal ion binding; cGMP binding

Biological Process: regulation of smooth muscle cell proliferation; cAMP catabolic process; cGMP catabolic process; signal transduction