Rabbit anti-Human Isocitrate Dehydrogenase Polyclonal Antibody | anti-ICDH antibody

Isocitrate Dehydrogenase, CT (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial [Precursor], Isocitric dehydrogenase, NAD(+)-specific ICDH, IDH3A, IDH3) (MaxLight 650)

Cat. Num. AAA6483631

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Isocitrate Dehydrogenase; Polyclonal Antibody; CT (Isocitrate dehydrogenase [NAD] subunit alpha; mitochondrial [Precursor]; Isocitric dehydrogenase; NAD(+)-specific ICDH; IDH3A; IDH3) (MaxLight 650); anti-ICDH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IDH3.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-ICDH antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human Isocitrate dehydrogenase (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Isocitrate dehydrogenase in MCF-7, Jurkat cell line lysates (35ug/lane). Isocitrate dehydrogenase (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-ICDH antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Soundar, S., et al., J. Biol. Chem. 278(52):52146-52153 (2003). 2. Weiss, C., et al., Biochemistry 39(7):1807-1816 (2000). 3. Kim, Y.O., et al., J. Biol. Chem. 274(52):36866-36875 (1999). 4. Huh, T.L., et al., Genomics 32(2):295-296 (1996). 5. Kim, Y.O., et al., Biochem. J. 308(PT 1), 63-68 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 5031777
• NCBI GeneID: 3419
• NCBI Accession #: NP_005521
• NCBI GenBank Nucleotide #: NM_005530
• UniProt Accession #: P50213; Q9H3X0; D3DW83
• Molecular Weight: 31,381 Da
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 3 (NAD+) alpha
• NCBI Official Symbol: IDH3A
• NCBI Protein Information: isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; H-IDH alpha; NAD(+)-specific ICDH subunit alpha; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH; isocitra
• UniProt Protein Name: Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH3A
• UniProt Entry Name: IDH3A_HUMAN

Uniprot Description

IDH3A: a enzyme that that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG), requiring nicotinamide adenine dinucleotide (NAD) as a cofactor. The holoenzyme is a heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1. IDH3 is a mitochondrial enzyme that functions in the Krebs cycle, which generates substrates for cellular respiration. Alpha-KG can exit the mitochondria and enter other cellular compartments where it is a cofactor for the dioxygenases that hydroxylate the transcription factor HIF and trigger its degradation by VHL. Since HIF turns on oncogenic pathways, IDH3 has apparent tumor suppressor activity. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase; EC 1.1.1.41; Mitochondrial

Chromosomal Location of Human Ortholog: 15q25.1-q25.2

Cellular Component: mitochondrion; mitochondrial matrix; nucleus

Molecular Function: magnesium ion binding; isocitrate dehydrogenase (NAD+) activity; NAD binding

Biological Process: cellular metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process