Rabbit ISL1 Polyclonal Antibody | anti-ISL1 antibody

ISL1 (IISL LIM Homeobox 1, Insulin Gene Enhancer Protein ISL-1, Isl-1, ISLET1, Islet-1)

Cat. Num. AAA623314

• Gene Names: ISL1; Isl-1; ISLET1
• Reactivity: Bovine, Canine, Equine, Human, Mammal, Rat, Sheep
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified.
• Synonyms: ISL1; Polyclonal Antibody; ISL1 (IISL LIM Homeobox 1; Insulin Gene Enhancer Protein ISL-1; Isl-1; ISLET1; Islet-1); Anti -ISL1 (IISL LIM Homeobox 1; anti-ISL1 antibody

• Host: Rabbit
• Reactivity: Bovine, Canine, Equine, Human, Mammal, Rat, Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ISL-1. Species Crossreactivity: mouse, hequine, bovine, sheep, rat, canine.
• Purity/Purification: Affinity Purified; Purified.
• Form/Format: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide

• Applicable Applications for anti-ISL1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry.; Dilution: Western Blot: 0.5 ug/mL detected ISL-1 on 10ug cell lysate.; Immunohistochemistry: 1:300 dilution from a previous lot detected ISL-1 in mouse pancreas tissue.
• Immunogen: Linear peptide corresponding to Human ISL-1(KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis K562 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with MBS623314 (1:1000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system. Arrow indicates ISL-1 (~35kD).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of ISL-1 staining pattern and morphology in mouse pancreas. Tissue was treated with citrate pH 6.0 antigen retrieval and probed with 1:300 dilution of MBS623314. Antigen was visualized using IHC-SelectTM Detection Kit which utilizes HRP-DAB staining. Immunoreactivity is seen in acinar cells with no intralobular duct cell involvement.)

Related Product Information for anti-ISL1 antibody

Islet-1 (ISL-1) is a transcription factor that plays an important role in the embryogenesis of pancreatic islets of Langerhans. It is expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. In mouse embryos, a deficiency of this gene fail to undergo neural tube motor neuron differentiation. More recently it has been identified as a marker for a cardiac progenitor cell lineage that is capable of differentiating into all 3 major heart cell types: cardiomyocytes, smooth muscle and endothelial cell lineages.

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Product Categories/Family for anti-ISL1 antibody

Antibodies; Abs to Transcription Factors; Homeobox (HOX)

NCBI and Uniprot Product Information

• NCBI GI #: 115387114
• NCBI GeneID: 3670
• NCBI Accession #: NP_002193.2
• NCBI GenBank Nucleotide #: NM_002202.2
• UniProt Accession #: P61371; P20663; P47894
• Molecular Weight: 39,036 Da
• NCBI Official Full Name: insulin gene enhancer protein ISL-1
• NCBI Official Synonym Full Names: ISL LIM homeobox 1
• NCBI Official Symbol: ISL1
• NCBI Official Synonym Symbols: Isl-1; ISLET1
• NCBI Protein Information: insulin gene enhancer protein ISL-1; islet-1; OTTHUMP00000221832; OTTHUMP00000221833; OTTHUMP00000221834; OTTHUMP00000221965; ISL1 transcription factor, LIM/homeodomain
• UniProt Protein Name: Insulin gene enhancer protein ISL-1
• Protein Family: Insulin gene enhancer protein
• UniProt Gene Name: ISL1
• UniProt Entry Name: ISL1_HUMAN

NCBI Description

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq]

Uniprot Description

ISL1: Binds to one of the cis-acting domain of the insulin gene enhancer.

Protein type: Cell development/differentiation; DNA-binding; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 5q11.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; zinc ion binding; bHLH transcription factor binding; estrogen receptor binding; chromatin binding

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of interleukin-12 production; positive regulation of interleukin-1 alpha production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of estrogen receptor signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of cell proliferation; pancreas development; ventricular cardiac muscle morphogenesis; neuron fate specification; negative regulation of neuron apoptosis; sensory system development; neural crest cell migration; positive regulation of DNA binding; trigeminal nerve development; pharyngeal system development; axon regeneration; spinal cord motor neuron cell fate specification; positive regulation of insulin secretion; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; spinal cord motor neuron differentiation; positive regulation of interleukin-1 beta production; negative regulation of neuron differentiation; positive regulation of angiogenesis; positive regulation of interferon-gamma production; visceral motor neuron differentiation; mesenchymal cell differentiation; pituitary gland development; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; peripheral nervous system neuron axonogenesis; retinal ganglion cell axon guidance