Western Blot (WB)
(Figure 1. Western blot analysis using MYL3 (1) and MYL2 (2) mouse mAb against rat fetal heart tissues lysate.)
Mouse anti-Human MYL3 Antibody | anti-MYL3 antibody
MYL3 Antibody
Gene Names
MYL3; CMH8; VLC1; VLCl; MLC1V; MLC1SB; MLC-lV/sb
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
MYL3; MYL3 Antibody; CMH8; VLC1; MLC1V; MLC1SB; anti-MYL3 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
3F8
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
195
Applicable Applications for anti-MYL3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000
IHC: 1:200-1:1000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of MYL3 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Western Blot (WB)
(Figure 1. Western blot analysis using MYL3 (1) and MYL2 (2) mouse mAb against rat fetal heart tissues lysate.)
Western Blot (WB)
(Figure 1. Western blot analysis using MYL3 (1) and MYL2 (2) mouse mAb against rat fetal heart tissues lysate.)
Immunohistochemistry (IHC)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human Heart tissues using MYL3 mouse mAb.)
Immunohistochemistry (IHC)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human Heart tissues using MYL3 mouse mAb.)
Related Product Information for anti-MYL3 antibody
Myosins are a large superfamily of motor proteins that move along actin filaments, while hydrolyzing ATP. Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains and four light chains. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Human myosin light chain has clinical application as a cardiac marker. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.
Product Categories/Family for anti-MYL3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22kDa
NCBI Official Full Name
myosin light chain 3
NCBI Official Synonym Full Names
myosin light chain 3
NCBI Official Symbol
MYL3
NCBI Official Synonym Symbols
CMH8; VLC1; VLCl; MLC1V; MLC1SB; MLC-lV/sb
NCBI Protein Information
myosin light chain 3
UniProt Protein Name
Myosin light chain 3
Protein Family
UniProt Gene Name
MYL3
UniProt Synonym Gene Names
MLC-lV/sb
NCBI Description
MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
Uniprot Description
Regulatory light chain of myosin. Does not bind calcium.
Research Articles on MYL3
Similar Products
Product Notes
The MYL3 myl3 (Catalog #AAA850600) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MYL3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYL3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the MYL3 myl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYL3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
