Rabbit anti-Human IRX2 Polyclonal Antibody | anti-IRX2 antibody

Rabbit Polyclonal (IgG) to Human IRX2

Cat. Num. AAA246714

• Gene Names: IRX2; IRXA2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: IRX2; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human IRX2; Anti-IRX2 Antibody (aa231-280) IHC-plus; IRXA2; Homeodomain protein IRXA2; Iroquois homeobox 2; Iroquois homeobox protein 2; Human IRX2; anti-IRX2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IRX2 Antibody detects endogenous levels of total IRX2 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 471

• Applicable Applications for anti-IRX2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000), IF (1:100 - 1:500), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human IRX2 (aa231-280).
• Immunogen: IRX2 antibody was raised against synthetic peptide from human IRX2 (aa231-280).
• Antigen Modification: aa231-280
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-IRX2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-IRX2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of HepG2 cells, using IRX2 Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from 293/HepG2 cells, using IRX2 Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 197100900
• NCBI GeneID: 153572
• NCBI Accession #: NP_001127694.1
• NCBI GenBank Nucleotide #: NM_001134222.1
• UniProt Accession #: Q9BZI1; Q68A19; Q7Z2I7
• Molecular Weight: 49,129 Da
• NCBI Official Full Name: iroquois-class homeodomain protein IRX-2
• NCBI Official Synonym Full Names: iroquois homeobox 2
• NCBI Official Symbol: IRX2
• NCBI Official Synonym Symbols: IRXA2
• NCBI Protein Information: iroquois-class homeodomain protein IRX-2; homeodomain protein IRXA2; iroquois homeobox protein 2
• UniProt Protein Name: Iroquois-class homeodomain protein IRX-2
• Protein Family: Iroquois-class homeodomain protein
• UniProt Gene Name: IRX2
• UniProt Synonym Gene Names: IRXA2
• UniProt Entry Name: IRX2_HUMAN

NCBI Description

IRX2 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.[supplied by OMIM, Apr 2004]

Uniprot Description

IRX2: Belongs to the TALE/IRO homeobox family

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: regulation of transcription, DNA-dependent