Western Blot
(Western Blot: C2C12 cells were infected with adenovirus GFP control (Ad-GFP) or HA-Irisin (Ad-Irisin) and cell lysates were subjected to western blot analysis by staining with our anti-Irisin antibody (500 ng/ml). Both the 18 and 21 kDa bands are irisin, resulting from multiple FNDC5-derived fragments in different cell lines and tissues due to alternative splicing and post-translation modifications. Antigen neutralization studies and FNDC5 knockout studies in HCC cells has been done to validate the protein bands detected by western blot in collaboration with Professor Ming-Hong Tai from National Sun Yat-sen University in Taiwan. The 25 kDa band shows the HA-tagged irisin.)
Rabbit anti-Mouse, Human Irisin (FNDC5) Polyclonal Antibody | anti-FNDC5 antibody
Rabbit Anti-Irisin (FNDC5) Affinity Pure
Centrifuge before use.
Western Blot
(Western Blot: C2C12 cells were infected with adenovirus GFP control (Ad-GFP) or HA-Irisin (Ad-Irisin) and cell lysates were subjected to western blot analysis by staining with our anti-Irisin antibody (500 ng/ml). Both the 18 and 21 kDa bands are irisin, resulting from multiple FNDC5-derived fragments in different cell lines and tissues due to alternative splicing and post-translation modifications. Antigen neutralization studies and FNDC5 knockout studies in HCC cells has been done to validate the protein bands detected by western blot in collaboration with Professor Ming-Hong Tai from National Sun Yat-sen University in Taiwan. The 25 kDa band shows the HA-tagged irisin.)
Western Blot
(Western Blot: C2C12 cells were infected with adenovirus GFP control (Ad-GFP) or HA-Irisin (Ad-Irisin) and cell lysates were subjected to western blot analysis by staining with our anti-Irisin antibody (500 ng/ml). Both the 18 and 21 kDa bands are irisin, resulting from multiple FNDC5-derived fragments in different cell lines and tissues due to alternative splicing and post-translation modifications. Antigen neutralization studies and FNDC5 knockout studies in HCC cells has been done to validate the protein bands detected by western blot in collaboration with Professor Ming-Hong Tai from National Sun Yat-sen University in Taiwan. The 25 kDa band shows the HA-tagged irisin.)
Immunohistochemistry (IHC)
(IHC: Paraffin-embedded mouse tissues were stained with anti-Irisin (1 ug/ml) by immunohistochemical analysis. The paraffin-embedded mice tissues were sectioned into 3-mum slices and mounted on poly (l-lysine)-coated slides. After deparaffinization, the slides were blocked with 3% hydrogen peroxide for 10 min and subjected to antigen retrieval with microwave in 10 mM citrate buffer for 15 min. The slides were incubated with irisin antibodies (1 mug/ml) at 4 degree C After wash with PBS, the sections were incubated with horseradish peroxidase/Fab polymer conjugate (polymer detection system; Zymed Laboratories, South San Francisco, CA) for 30 min and detected using diaminobenzidine (1:20 dilution; Zymed Laboratories).)
Immunohistochemistry (IHC)
(IHC: Paraffin-embedded mouse tissues were stained with anti-Irisin (1 ug/ml) by immunohistochemical analysis. The paraffin-embedded mice tissues were sectioned into 3-mum slices and mounted on poly (l-lysine)-coated slides. After deparaffinization, the slides were blocked with 3% hydrogen peroxide for 10 min and subjected to antigen retrieval with microwave in 10 mM citrate buffer for 15 min. The slides were incubated with irisin antibodies (1 mug/ml) at 4 degree C After wash with PBS, the sections were incubated with horseradish peroxidase/Fab polymer conjugate (polymer detection system; Zymed Laboratories, South San Francisco, CA) for 30 min and detected using diaminobenzidine (1:20 dilution; Zymed Laboratories).)
Immunofluorescence (IF)
(IF: Immunofluorescence analysis of FNDC5 expression in cardiomyocytes and endothelial cells. Cultured cells were fixed, permeabilized, then stained with anti-Irisin (1 ug/ml) by immunofluorescence analysis. After fixation, the cells were permeabilized then stained with anti-Irisin (1 mug/ml) at room temperature for 1 h followed by repeated washes with PBS. Then, the slides were incubated with fluorescein 5-isothiocyanate (FITC)-labeled secondary antibody (1?500 dilution) for 30 min at room temperature. After mounting in anti-Fade media, the slides were visualized under a fluorescence microscope.)
Immunofluorescence (IF)
(IF: Immunofluorescence analysis of FNDC5 expression in cardiomyocytes and endothelial cells. Cultured cells were fixed, permeabilized, then stained with anti-Irisin (1 ug/ml) by immunofluorescence analysis. After fixation, the cells were permeabilized then stained with anti-Irisin (1 mug/ml) at room temperature for 1 h followed by repeated washes with PBS. Then, the slides were incubated with fluorescein 5-isothiocyanate (FITC)-labeled secondary antibody (1?500 dilution) for 30 min at room temperature. After mounting in anti-Fade media, the slides were visualized under a fluorescence microscope.)
ELISA (EIA)
(ELISA: Data demonstrates detection of irisin after gene delivery following ELISA protocol)
ELISA (EIA)
(ELISA: Data demonstrates detection of irisin after gene delivery following ELISA protocol)
Use: The antibody can be used for detection of Irisin in plasma, immunosassays, immunoblots.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Uniprot Description
Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.
Research Articles on FNDC5
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Product Notes
The FNDC5 fndc5 (Catalog #AAA390152) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Irisin (FNDC5) Affinity Pure reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Irisin (FNDC5) can be used in a range of immunoassay formats including, but not limited to, Immunoblot (IB), ELISA (EIA). Dilution for Immunoblot & ELISA range: 1,000 to 4,000. Centrifuge before use. Researchers should empirically determine the suitability of the FNDC5 fndc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Irisin (FNDC5), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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