Rabbit IRF8 Polyclonal Antibody | anti-IRF3 antibody

IRF8 Antibody

Cat. Num. AAA150591

• Gene Names: IRF8; ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: IRF8 Antibody is affinity chromatography purified via peptide column.
• Synonyms: IRF8; Polyclonal Antibody; IRF8 Antibody; Interferon regulatory factor 3; IRF-3; interferon regulatory factor 3; anti-IRF3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: IRF8 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 426

• Applicable Applications for anti-IRF3 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: IRF8 antibody can be used for detection of IRF8 by Western blot at 1 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: IRF8 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRF8.
• Buffer: IRF8 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: IRF8 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of IRF8 in human thymus tissue lysate with IRF8 antibody at 1μg/mL in (A) the presence and (B) absence of blocking peptide.)

Immunofluorescence (IF)

(Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 μg/mL.)

Related Product Information for anti-IRF3 antibody

IRF8 Antibody: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Unlike IRF3, IRF8 appears to act as a negative regulator of IFN-induced genes in most cases, but IRF8 mediates activation of NF-κB by the toll-like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia.

NCBI and Uniprot Product Information

• NCBI GI #: 4504567
• NCBI GeneID: 3394
• NCBI Accession #: NP_002154
• NCBI GenBank Nucleotide #: NM_002163.2
• UniProt Accession #: Q14653; A0AV82; Q02556
• Molecular Weight: 48,356 Da
• NCBI Official Full Name: interferon regulatory factor 8
• NCBI Official Synonym Full Names: interferon regulatory factor 8
• NCBI Official Symbol: IRF8
• NCBI Official Synonym Symbols: ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP
• NCBI Protein Information: interferon regulatory factor 8; interferon consensus sequence binding protein 1
• UniProt Protein Name: Interferon regulatory factor 8
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF8
• UniProt Synonym Gene Names: ICSBP1; IRF-8; H-ICSBP; ICSBP
• UniProt Entry Name: IRF8_HUMAN

NCBI Description

Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq, Jul 2008]

Uniprot Description

IRF3: interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription cofactor activity; transcription factor activity

Biological Process: negative regulation of interferon-beta biosynthetic process; transcription from RNA polymerase II promoter; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; negative regulation of defense response to virus by host; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; response to exogenous dsRNA; positive regulation of interferon type I production; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; positive regulation of interferon-beta production; innate immune response; interferon type I biosynthetic process; toll-like receptor 4 signaling pathway; response to DNA damage stimulus; negative regulation of interferon type I production; defense response to virus; positive regulation of interferon-alpha production; positive regulation of cytokine secretion

Disease: Herpes Simplex Encephalitis, Susceptibility To, 7