Western Blot (WB)
(Staining (0.2ug/ml) of Human Ovary lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat IRF6 Polyclonal Antibody | anti-IRF6 antibody
Goat anti-IRF6 Antibody
Immunohistochemistry: In paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulus. Recommended concentration, 2-3ug/ml.
Western Blot: Approx 60kDa band observed in Human Ovary lysates (calculated MW of 53.1kDa according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx.55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.2-0.6ug/ml.
Western Blot (WB)
(Staining (0.2ug/ml) of Human Ovary lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.2ug/ml) of Human Ovary lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing IRF6 (RC201579) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing IRF6 (RC201579) and probed (mock transfection in first lane), tested by Origene.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2011]
Uniprot Description
IRF6: Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development. Plays a role in regulating mammary epithelial cell proliferation. May regulate WDR65 transcription. Interacts with SERPINB5. Expressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas. Belongs to the IRF family.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 1q32.3-q41
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: DNA binding; protein binding; transcription factor activity
Biological Process: cell cycle arrest; cell development; keratinocyte differentiation; keratinocyte proliferation; negative regulation of cell proliferation; positive regulation of transcription, DNA-dependent; transcription, DNA-dependent
Disease: Orofacial Cleft 6, Susceptibility To; Popliteal Pterygium Syndrome; Van Der Woude Syndrome 1
Research Articles on IRF6
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Product Notes
The IRF6 irf6 (Catalog #AAA420241) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-IRF6 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IRF6 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulus. Recommended concentration, 2-3ug/ml. Western Blot: Approx 60kDa band observed in Human Ovary lysates (calculated MW of 53.1kDa according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx.55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.2-0.6ug/ml. Researchers should empirically determine the suitability of the IRF6 irf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TPSMQLPPAL PPQ. It is sometimes possible for the material contained within the vial of "IRF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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