Rabbit anti-Mouse Irf3 Polyclonal Antibody | anti-Irf3 antibody

Irf3, NT (Irf3, Interferon regulatory factor 3) (PE)

Cat. Num. AAA6310926

• Gene Names: Irf3; IRF-3; C920001K05Rik
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Irf3; Polyclonal Antibody; NT (Irf3; Interferon regulatory factor 3) (PE); Interferon regulatory factor 3; anti-Irf3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Irf3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Irf3 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 46-72 in the N-terminal region of mouse Irf3, UniProt Accession #NP_058545.1; P70671; Q764M6; Q4JF28. Species sequence homology: bovine, porcine
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS6005980. This demonstrates that MBS6005980 detected the MOUSE Irf3 protein (arrow).)

Related Product Information for anti-Irf3 antibody

Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains (By similarity).

Product Categories/Family for anti-Irf3 antibody

Antibodies; Interferon

References

1. Marichal, T., et al. J. Allergy Clin. Immunol. 126(4):836-844(2010). 2. Menachery, V.D., et al. J. Virol. 84(19):9685-9694(2010). 3. Carrigan, S.O., et al. J. Immunol. 85(6):3602-3609(2010). 4. Wang, J., et al. J. Immunol. 185(3):1720-1729(2010). 5. Farlik, M., et al. Immunity 33(1):25-34(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 8393627
• NCBI GeneID: 54131
• NCBI Accession #: NP_058545.1
• NCBI GenBank Nucleotide #: NM_016849.4
• UniProt Accession #: P70671; Q764M6; Q4JF28
• Molecular Weight: 46,852 Da
• NCBI Official Full Name: interferon regulatory factor 3
• NCBI Official Synonym Full Names: interferon regulatory factor 3
• NCBI Official Symbol: Irf3
• NCBI Official Synonym Symbols: IRF-3; C920001K05Rik
• NCBI Protein Information: interferon regulatory factor 3
• UniProt Protein Name: Interferon regulatory factor 3
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: Irf3
• UniProt Synonym Gene Names: IRF-3

Uniprot Description

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.