Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western BlotPositive WB detected in: Rat liver tissueAll lanes: USF2 antibody at 4.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37, 31, 24 KDaObserved band size: 37 KDa)

Rabbit anti-Human, Rat USF2 Polyclonal Antibody | anti-USF2 antibody

USF2 Antibody

Gene Names
USF2; FIP; bHLHb12
Reactivity
Human, Rat
Applications
ELISA, Western Blot, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
USF2; Polyclonal Antibody; USF2 Antibody; Upstream stimulatory factor 2; Class B basic helix-loop-helix protein 12; bHLHb12; FOS-interacting protein; FIP; Major late transcription factor 2; Upstream transcription factor 2; BHLHB12; anti-USF2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
215
Applicable Applications for anti-USF2 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IF: 1:50-1:200
Immunogen
Recombinant Human Upstream stimulatory factor 2 protein (21-162AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Rat liver tissueAll lanes: USF2 antibody at 4.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37, 31, 24 KDaObserved band size: 37 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Rat liver tissueAll lanes: USF2 antibody at 4.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37, 31, 24 KDaObserved band size: 37 KDa)

Immunofluorescence (IF)

(Immunofluorescence staining of MCF-7 cells with MBS7107259 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Immunofluorescence (IF) (Immunofluorescence staining of MCF-7 cells with MBS7107259 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-USF2 antibody
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Product Categories/Family for anti-USF2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,988 Da
NCBI Official Full Name
upstream stimulatory factor 2 isoform 3
NCBI Official Synonym Full Names
upstream transcription factor 2, c-fos interacting
NCBI Official Symbol
USF2
NCBI Official Synonym Symbols
FIP; bHLHb12
NCBI Protein Information
upstream stimulatory factor 2
UniProt Protein Name
Upstream stimulatory factor 2
UniProt Gene Name
USF2
UniProt Synonym Gene Names
BHLHB12; bHLHb12; FIP

NCBI Description

This gene encodes a member of the basic helix-loop-helix leucine zipper family of transcription factors. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs and is involved in regulating multiple cellular processes. [provided by RefSeq, Mar 2016]

Uniprot Description

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

Research Articles on USF2

Similar Products

Product Notes

The USF2 usf2 (Catalog #AAA7107259) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USF2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's USF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:5000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the USF2 usf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.