Rabbit anti-Human CTSS Polyclonal Antibody | anti-CTSS antibody

CTSS, ID (Cathepsin S) (MaxLight 405)

Cat. Num. AAA6467757

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CTSS; Polyclonal Antibody; ID (Cathepsin S) (MaxLight 405); anti-CTSS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CTSS.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-CTSS antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CTSS (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CTSS antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). CTSS (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CTSS Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CTSS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CTSS Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CTSS antibody

Antibodies; Cathepsin

References

1. Lindahl, C., Cancer Genomics Proteomics 6(3), 149-159 (2009). 2. Bunatova, K., Int. J. Biol. Markers 24(1), 47-51 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 23110962
• NCBI GeneID: 1520
• NCBI Accession #: NP_004070
• NCBI GenBank Nucleotide #: NM_004079.4
• UniProt Accession #: P25774; Q5T5I0; Q6FHS5; Q9BUG3; B4DWC9; D3DV05
• Molecular Weight: 31,626 Da
• NCBI Official Full Name: cathepsin S isoform 1 preproprotein
• NCBI Official Synonym Full Names: cathepsin S
• NCBI Official Symbol: CTSS
• NCBI Protein Information: cathepsin S
• UniProt Protein Name: Cathepsin S
• Protein Family: Cathepsin
• UniProt Gene Name: CTSS
• UniProt Entry Name: CATS_HUMAN

NCBI Description

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

CTSS: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Belongs to the peptidase C1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.27

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: lysosomal lumen; extracellular space; intracellular membrane-bound organelle; membrane; lysosome; extracellular region

Molecular Function: collagen binding; proteoglycan binding; cysteine-type endopeptidase activity; fibronectin binding; laminin binding

Biological Process: adaptive immune response; extracellular matrix organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis; collagen catabolic process; antigen processing and presentation; extracellular matrix disassembly; antigen processing and presentation of peptide antigen via MHC class I; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; positive regulation of inflammatory response