Western Blot (WB)
((0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat IREB2/IRP2 Polyclonal Antibody | anti-IREB2 antibody
Goat anti-IREB2/IRP2 Antibody
Gene Names
IREB2; ACO3; IRP2; IRP2AD
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
IREB2/IRP2; Polyclonal Antibody; Goat anti-IREB2/IRP2 Antibody; IREB2; iron-responsive element binding protein 2; ACO3; FLJ23381; IRP2; IRP2AD; iron regulatory protein 2; IREB2 antibody; iron-responsive element binding protein 2 antibody; ACO3 antibody; FLJ23381 antibody; IRP2 antibody; IRP2AD antibody; iron regulatory protein 2 antibody; IREB2 / IRP2; anti-IREB2 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SIHYEGSEYKLSHGS
Sequence Length
963
Applicable Applications for anti-IREB2 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Kidney shows preferential staining in the cytoplasm of DCT. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 110kDa band observed in Human Liver lysates (calculated MW of 105kDa according to NP_004127.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was confirmed as a breakdown product in aged purified recombinant protein by a free sample recipient.
Immunohistochemistry: In paraffin embedded Human Kidney shows preferential staining in the cytoplasm of DCT. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 110kDa band observed in Human Liver lysates (calculated MW of 105kDa according to NP_004127.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was confirmed as a breakdown product in aged purified recombinant protein by a free sample recipient.
Immunogen
Peptide with sequence C-SIHYEGSEYKLSHGS, from the internal region of the protein sequence according to NP_004127.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,487 Da
NCBI Official Full Name
iron-responsive element-binding protein 2 isoform 1
NCBI Official Synonym Full Names
iron responsive element binding protein 2
NCBI Official Symbol
IREB2
NCBI Official Synonym Symbols
ACO3; IRP2; IRP2AD
NCBI Protein Information
iron-responsive element-binding protein 2
UniProt Protein Name
Iron-responsive element-binding protein 2
Protein Family
UniProt Gene Name
IREB2
UniProt Synonym Gene Names
IRE-BP 2; IRP2
UniProt Entry Name
IREB2_HUMAN
Uniprot Description
IREB2: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'- UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. Belongs to the aconitase/IPM isomerase family.
Protein type: RNA-binding; Translation
Chromosomal Location of Human Ortholog: 15q25.1
Cellular Component: cytoplasm
Molecular Function: 4 iron, 4 sulfur cluster binding; iron-responsive element binding; metal ion binding; protein binding; RNA binding; translation repressor activity
Biological Process: cellular iron ion homeostasis; iron ion transport; metabolic process; negative regulation of translation
Research Articles on IREB2
Similar Products
Product Notes
The IREB2 ireb2 (Catalog #AAA421955) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-IREB2/IRP2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IREB2/IRP2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Kidney shows preferential staining in the cytoplasm of DCT. Recommended concentration, 3-6ug/ml. Western Blot: Approx 110kDa band observed in Human Liver lysates (calculated MW of 105kDa according to NP_004127.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was confirmed as a breakdown product in aged purified recombinant protein by a free sample recipient. Researchers should empirically determine the suitability of the IREB2 ireb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SIHYEGSEYK LSHGS. It is sometimes possible for the material contained within the vial of "IREB2/IRP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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