Western Blot (WB)
(Host: RabbitTarget Name: IREB2Sample Tissue: Human Fetal LungAntibody Dilution: 1.0ug/ml)
Rabbit IREB2 Polyclonal Antibody | anti-IREB2 antibody
IREB2 antibody - middle region
Western Blot (WB)
(Host: RabbitTarget Name: IREB2Sample Tissue: Human Fetal LungAntibody Dilution: 1.0ug/ml)
Western Blot (WB)
(Host: RabbitTarget Name: IREB2Sample Tissue: Human Fetal LungAntibody Dilution: 1.0ug/ml)
Western Blot (WB)
(Researcher: Dr. Hao Zhu, University of Kansas Medical CenterApplication: Western blottingSpecies+tissue/cell type: Mouse liver extractHow many ug'sof tissue/cell lysate run on the gel:1. 60 ug mouse liver extract2. 60 ug mouse liver extract3. 60 ug mouse liver extractPrimary antibody dilution: 1:500Secondary antibody: Anti-rabbit HRPSecondary antibody dilution: 1:3000)
Western Blot (WB)
(Researcher: Dr. Hao Zhu, University of Kansas Medical CenterApplication: Western blottingSpecies+tissue/cell type: Mouse liver extractHow many ug'sof tissue/cell lysate run on the gel:1. 60 ug mouse liver extract2. 60 ug mouse liver extract3. 60 ug mouse liver extractPrimary antibody dilution: 1:500Secondary antibody: Anti-rabbit HRPSecondary antibody dilution: 1:3000)
Western Blot (WB)
(WB Suggested Anti-IREB2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Liver)
Western Blot (WB)
(WB Suggested Anti-IREB2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Liver)
Target Description: IREB2 binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IREB2 binds to the IRE element in ferritin which results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner. Variants in this gene have been associated with lung cancer and chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
Uniprot Description
IREB2: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'- UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. Belongs to the aconitase/IPM isomerase family.
Protein type: RNA-binding; Translation
Chromosomal Location of Human Ortholog: 15q25.1
Cellular Component: mitochondrion; cytoplasm; cytosol
Molecular Function: protein binding; iron-responsive element binding; translation repressor activity; RNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding
Biological Process: erythrocyte homeostasis; response to retinoic acid; cellular iron ion homeostasis; negative regulation of translation; response to iron(II) ion; osteoclast differentiation; iron ion transport; protoporphyrinogen IX biosynthetic process; intestinal absorption; aging; post-embryonic development
Research Articles on IREB2
Similar Products
Product Notes
The IREB2 ireb2 (Catalog #AAA3205284) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IREB2 antibody - middle region reacts with Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's IREB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the IREB2 ireb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: IQINLNSIVP SVSGPKRPQD RVAVTDMKSD FQACLNEKVG FKGFQIAAEK. It is sometimes possible for the material contained within the vial of "IREB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
