Rabbit anti-Human IREB2 Polyclonal Antibody | anti-IREB2 antibody

IREB2 Polyclonal Antibody

Cat. Num. AAA3011533

• Reactivity: Human
• Applications: Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IREB2; Polyclonal Antibody; IREB2 Polyclonal Antibody; Iron-responsive element-binding protein 2; IRE-BP 2; Iron regulatory protein 2; IRP2; anti-IREB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IREB2 polyclonal antibody detects endogenous levels of IREB2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 963

• Applicable Applications for anti-IREB2 antibody: Immunofluorescence (IF)
• Application Notes: IF: 1:20-1:50
• Immunogen: Recombinant full length Human IREB2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using IREB2 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-IREB2 antibody

-Iron metabolism is essential for sustaining mammalian homeostasis. Iron uptake and distribution is a highly regulated process in mammalian cells that is monitored by two iron sensing proteins: IRP-1 and -2 (iron regulatory protein-1 and -2), also known as iron responsive element-binding protein-1 and -2 (IRE-BP-1 and -2) or aconitase 1 and 2. IRP-1 and IRP-2 are important soluble regulatory factors that mediate iron uptake and storage in mammalian cells. They are capable of either repressing translation or enhancing mRNA stability by associating with stem-loop motifs known as iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide.

NCBI and Uniprot Product Information

• NCBI GI #: 41352693
• NCBI Accession #: NP_004127.1
• NCBI GenBank Nucleotide #: NP_004127.1
• UniProt Accession #: P48200
• Molecular Weight: ~ 105kDa
• NCBI Official Full Name: iron-responsive element-binding protein 2 isoform 1
• UniProt Protein Name: Iron-responsive element-binding protein 2
• Protein Family: Iron-responsive element-binding protein
• UniProt Gene Name: IREB2
• UniProt Synonym Gene Names: IRE-BP 2; IRP2
• UniProt Entry Name: IREB2_HUMAN

Uniprot Description

IREB2: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'- UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. Belongs to the aconitase/IPM isomerase family.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: mitochondrion; cytoplasm; cytosol

Molecular Function: protein binding; iron-responsive element binding; translation repressor activity; RNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding

Biological Process: erythrocyte homeostasis; response to retinoic acid; cellular iron ion homeostasis; negative regulation of translation; response to iron(II) ion; osteoclast differentiation; iron ion transport; protoporphyrinogen IX biosynthetic process; intestinal absorption; aging; post-embryonic development