Rabbit IREB2 Polyclonal Antibody | anti-IREB2 antibody
IREB2 Antibody
Gene Names
IREB2; ACO3; IRP2; IRP2AD
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
IREB2; Polyclonal Antibody; IREB2 Antibody; ACO3; FLJ23381; IRE BP2; IRE-BP 2; IREB 2; IREB2_HUMAN; Iron regulatory protein 2; iron responsive element binding protein 2; Iron-responsive element-binding protein 2; IRP 2; IRP2; IRP2AD; OTTHUMP00000185022; anti-IREB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
IREB2 Antibody detects endogenous levels of total IREB2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IREB2 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from Human MYOCD.
Conjugation
Unconjugated
Similarity
Belongs to the aconitase/IPM isomerase family.
Post Translational Modifications
Ubiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity.
Subunit Structure
Interacts with RBCK1 isoform 1 and isoform 2 only in iron-rich conditions. Interacts (when associated with the 4Fe-4S) with FBXL5. Interacts with CIAO1 and CIAO2A.
Function
RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,487 Da
NCBI Official Full Name
iron-responsive element-binding protein 2
NCBI Official Synonym Full Names
iron-responsive element binding protein 2
NCBI Official Symbol
IREB2
NCBI Official Synonym Symbols
ACO3; IRP2; IRP2AD
NCBI Protein Information
iron-responsive element-binding protein 2; IRE-BP 2; iron regulatory protein 2
UniProt Protein Name
Iron-responsive element-binding protein 2
Protein Family
UniProt Gene Name
IREB2
UniProt Synonym Gene Names
IRE-BP 2; IRP2
UniProt Entry Name
IREB2_HUMAN
Uniprot Description
IREB2: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'- UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. Belongs to the aconitase/IPM isomerase family.
Protein type: RNA-binding; Translation
Chromosomal Location of Human Ortholog: 15q25.1
Cellular Component: mitochondrion; cytoplasm; cytosol
Molecular Function: protein binding; iron-responsive element binding; translation repressor activity; RNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding
Biological Process: erythrocyte homeostasis; response to retinoic acid; cellular iron ion homeostasis; negative regulation of translation; response to iron(II) ion; osteoclast differentiation; iron ion transport; protoporphyrinogen IX biosynthetic process; intestinal absorption; aging; post-embryonic development
Research Articles on IREB2
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Product Notes
The IREB2 ireb2 (Catalog #AAA9629724) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IREB2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IREB2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the IREB2 ireb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IREB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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