Rabbit IQGAP1 Polyclonal Antibody | anti-IQGAP1 antibody

IQGAP1 Polyclonal Antibody

Cat. Num. AAA3005931

• Gene Names: IQGAP1; SAR1; p195; HUMORFA01
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IQGAP1; Polyclonal Antibody; IQGAP1 Polyclonal Antibody; Ras GTPase-activating-like protein IQGAP1; p195; KIAA0051; anti-IQGAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IQGAP1 polyclonal antibody detects endogenous levels of IQGAP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1657

• Applicable Applications for anti-IQGAP1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 291-336 of Human IQGAP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of IQGAP1 polyclonal antibody at 1:500 dilutionLane1:MCF-7 cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-IQGAP1 antibody

IQGAP1, for IQ motif containing GTPase activating protein, is a Ras GAP-related, Actin-binding protein that interacts with the small GTPases Cdc42 and Rac1. The C-terminus of IQGAP1 is essential for interacting with Cdc42 and, in addition, IQGAP1 contains a WW domain and a predicted N-terminal coiled-coil region, which may be involved in IQGAP dimerization. Expression of IQGAP1 is highest in placenta, lung and kidney, where it co-localizes with Cdc42 to the cytoskeleton and assists with Cdc42 in mediating the regulation of cell proliferation, polarity and cell morphology. IQGAP1 regulates cadherin-mediated cell adhesion via binding to E-cadherin, b-catenin and a-catenin. This association induces the accumulation of these proteins at the site of cell-cell contact. IQGAP1 is negatively regulated by calmodulin, which binds to IQGAP1 in a calcium-dependent manner and disrupts IQGAP1 from associating with Cdc42.

NCBI and Uniprot Product Information

• NCBI GI #: 4506787
• NCBI GeneID: 8826
• NCBI Accession #: NP_003861.1
• NCBI GenBank Nucleotide #: NP_003861.1
• UniProt Accession #: P46940
• Molecular Weight: ~ 190kDa
• NCBI Official Full Name: ras GTPase-activating-like protein IQGAP1
• NCBI Official Synonym Full Names: IQ motif containing GTPase activating protein 1
• NCBI Official Symbol: IQGAP1
• NCBI Official Synonym Symbols: SAR1; p195; HUMORFA01
• NCBI Protein Information: ras GTPase-activating-like protein IQGAP1
• UniProt Protein Name: Ras GTPase-activating-like protein IQGAP1
• Protein Family: Ras GTPase-activating-like protein
• UniProt Gene Name: IQGAP1
• UniProt Synonym Gene Names: KIAA0051
• UniProt Entry Name: IQGA1_HUMAN

NCBI Description

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008]

Uniprot Description

IQGAP1: Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6. Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.

Protein type: GAPs, Ras; GAPs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: microtubule; neuron projection; focal adhesion; leading edge; actin filament; actin cytoskeleton; microtubule cytoskeleton; nucleoplasm; extrinsic to internal side of plasma membrane; growth cone; axon; cytoplasm; plasma membrane; midbody; cell junction; lateral plasma membrane

Molecular Function: calmodulin binding; protein serine/threonine kinase activator activity; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; Rac GTPase binding; protein complex binding; calcium ion binding; protein phosphatase binding; protein kinase binding; GTPase activator activity; GTPase inhibitor activity

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; small GTPase mediated signal transduction; positive regulation of protein kinase activity; negative regulation of dephosphorylation; negative regulation of catalytic activity; energy reserve metabolic process; platelet-derived growth factor receptor signaling pathway; signal transduction; regulation of insulin secretion; regulation of cytokine production; positive regulation of GTPase activity