Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Intestinal Cell Kinase Polyclonal Antibody | anti-ICK antibody
Polyclonal Antibody to Intestinal Cell Kinase (ICK)
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Intestinal Cell Kinase; Polyclonal Antibody; Polyclonal Antibody to Intestinal Cell Kinase (ICK); anti-ICK antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ICK. It has beenselected for its ability to recognize ICK in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
632
Applicable Applications for anti-ICK antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
ICK (Met1~Phe284)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Rat
Conjugate
No Conjugate
Immunogen
Recombinant ICK (Met1~Phe284) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2066514
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
70,569 Da
NCBI Official Full Name
serine/threonine-protein kinase ICK
NCBI Official Synonym Full Names
intestinal cell kinase
NCBI Official Symbol
Ick
NCBI Protein Information
serine/threonine-protein kinase ICK
UniProt Protein Name
Serine/threonine-protein kinase ICK
Protein Family
UniProt Gene Name
Ick
UniProt Synonym Gene Names
MRK
NCBI Description
may be involved in promoting meiosis during spermatogenesis [RGD, Feb 2006]
Uniprot Description
ICK: a CMGC kinase of the RCK family. May play a role in cardiac development. Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Also expressed in many cancer cell lines. Two alternatively spliced isoforms have been described.
Protein type: CMGC group; EC 2.7.11.22; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); RCK family
Chromosomal Location of Human Ortholog: 8q31
Cellular Component: cilium; cytoplasm; nucleus
Molecular Function: ATP binding; magnesium ion binding; metal ion binding; protein serine/threonine kinase activity
Biological Process: intraflagellar transport; multicellular organism development; protein amino acid phosphorylation; regulation of gene expression
Similar Products
Product Notes
The ICK ick (Catalog #AAA2026744) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Intestinal Cell Kinase (ICK) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Intestinal Cell Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the ICK ick for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Intestinal Cell Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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