Mouse Enhancer Of Zeste Homolog 1 (EZH1) ELISA Kit | EZH1 elisa kit

Mouse Enhancer Of Zeste Homolog 1 (EZH1) ELISA Kit

Cat. Num. AAA454897

• Gene Names: Ezh1; ENX-2
• Reactivity: Mouse
• Synonyms: Enhancer Of Zeste Homolog 1 (EZH1); Mouse Enhancer Of Zeste Homolog 1 (EZH1) ELISA Kit; EZH1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of EZH1. No significant cross-reactivity or interference between EZH1 and analogues was observed.
• Sequence Length: 750

• Samples: Mouse tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.061ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EZH1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EZH1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EZH1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of EZH1 in mouse tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EZH1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EZH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EZH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EZH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6679719
• NCBI GeneID: 14055
• NCBI Accession #: NP_031996.1
• NCBI GenBank Nucleotide #: NM_007970.3
• UniProt Accession #: P70351; Q3TPR1; Q3U3V5; Q3UU02; Q8BR85; Q922L1; Q9R089; A2A4K5
• Molecular Weight: 65,730 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase EZH1
• NCBI Official Synonym Full Names: enhancer of zeste 1 polycomb repressive complex 2 subunit
• NCBI Official Symbol: Ezh1
• NCBI Official Synonym Symbols: ENX-2
• NCBI Protein Information: histone-lysine N-methyltransferase EZH1
• UniProt Protein Name: Histone-lysine N-methyltransferase EZH1
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: Ezh1
• UniProt Synonym Gene Names: Enx2

NCBI Description

This gene encodes a member of the Polycomb-group (PcG) family. The encoded protein is interchangeable with the related Enhancer of zeste 2 (Ezh2) protein as a core component of the polycomb repressive complex 2 (PRC2), which methylates histone H3 at lysine 27 and results in the transcriptional repression of affected target genes. This complex is involved in carrying out cell-fate decisions during embryonic stem cell differentiation. [provided by RefSeq, Sep 2014]

Uniprot Description

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.