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Western Blot (WB) (Western Blot: Sample: Recombinant IL16, Human.)

Rabbit anti-Human Interleukin 16 (IL16) Polyclonal Antibody | anti-IL16 antibody

Polyclonal Antibody to Interleukin 16 (IL16)

Gene Names
IL16; LCF; NIL16; PRIL16; prIL-16
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Interleukin 16 (IL16); Polyclonal Antibody; Polyclonal Antibody to Interleukin 16 (IL16); anti-IL16 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against IL16. It has been selected for its ability to recognize IL16 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MPDLNSST DSAASASAAS DVSVESTAEA TVCTVTLEKM SAGLGFSLEG GKGSLHGDKP LTINRIFKGA ASEQSETVQP
Sequence Length
130
Applicable Applications for anti-IL16 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant IL16 (Met1203~Ser1332) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2036471
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant IL16, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant IL16, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Skin Cancer Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Skin Cancer Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Stomach Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,345 Da
NCBI Official Full Name
pro-interleukin-16 isoform 3
NCBI Official Synonym Full Names
interleukin 16
NCBI Official Symbol
IL16
NCBI Official Synonym Symbols
LCF; NIL16; PRIL16; prIL-16
NCBI Protein Information
pro-interleukin-16
UniProt Protein Name
Pro-interleukin-16
Protein Family
UniProt Gene Name
IL16
UniProt Synonym Gene Names
IL-16; LCF

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: cytosol; extracellular region; extracellular space; nuclear speck; plasma membrane

Molecular Function: CD4 receptor binding; cytokine activity

Biological Process: immune response; induction of positive chemotaxis; leukocyte chemotaxis; regulation of calcium ion transport; regulation of receptor activity; regulation of transcription, DNA-templated; transcription, DNA-dependent; viral process

Research Articles on IL16

Similar Products

Product Notes

The IL16 il16 (Catalog #AAA2017446) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interleukin 16 (IL16) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 16 (IL16) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the IL16 il16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MPDLNSST DSAASASAAS DVSVESTAEA TVCTVTLEKM SAGLGFSLEG GKGSLHGDKP LTINRIFKGA ASEQSETVQP. It is sometimes possible for the material contained within the vial of "Interleukin 16 (IL16), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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