Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Interleukin 12 Receptor Beta 2 (IL12Rb2) Polyclonal Antibody | anti-IL12Rb2 antibody
Polyclonal Antibody to Interleukin 12 Receptor Beta 2 (IL12Rb2)
Gene Names
Il12rb2; Ifnm; IL-12RB2; A930027I18Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Interleukin 12 Receptor Beta 2 (IL12Rb2); Polyclonal Antibody; Polyclonal Antibody to Interleukin 12 Receptor Beta 2 (IL12Rb2); anti-IL12Rb2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against IL12Rb2. It has been selected for its ability to recognize IL12Rb2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- TWQPPK KADSAVREYI VEWRALQPGS ITKFPPHWLR IPPDNMSALI SENIKPYICY EIRVHALSES QGGCSSIRGD SKHKAPVSGP HITAITEKKE RLFISWTHIP FPEQRGCILH YRIYWKERDS TAQPELCEIQ YRRSQNSHPI SSLQPR
MGHHHHHHSGSEF- TWQPPK KADSAVREYI VEWRALQPGS ITKFPPHWLR IPPDNMSALI SENIKPYICY EIRVHALSES QGGCSSIRGD SKHKAPVSGP HITAITEKKE RLFISWTHIP FPEQRGCILH YRIYWKERDS TAQPELCEIQ YRRSQNSHPI SSLQPR
Sequence Length
861
Applicable Applications for anti-IL12Rb2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant IL12Rb2 (Thr455~Arg606) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2051247
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
98,197 Da
NCBI Official Full Name
interleukin-12 receptor subunit beta-2 isoform 2
NCBI Official Synonym Full Names
interleukin 12 receptor, beta 2
NCBI Official Symbol
Il12rb2
NCBI Official Synonym Symbols
Ifnm; IL-12RB2; A930027I18Rik
NCBI Protein Information
interleukin-12 receptor subunit beta-2
UniProt Protein Name
Interleukin-12 receptor subunit beta-2
Protein Family
UniProt Gene Name
Il12rb2
UniProt Synonym Gene Names
IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2
Uniprot Description
IL12RB2: Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. Belongs to the type I cytokine receptor family. Type 2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 6 C1|6 30.81 cM
Cellular Component: external side of plasma membrane; integral component of membrane; membrane
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protein kinase binding
Biological Process: interferon-gamma production; peptidyl-tyrosine phosphorylation; positive regulation of interferon-gamma production; response to cytokine; response to lipopolysaccharide
Research Articles on IL12Rb2
Similar Products
Product Notes
The IL12Rb2 il12rb2 (Catalog #AAA2007231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interleukin 12 Receptor Beta 2 (IL12Rb2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 12 Receptor Beta 2 (IL12Rb2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the IL12Rb2 il12rb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- TWQPPK KADSAVREYI VEWRALQPGS ITKFPPHWLR IPPDNMSALI SENIKPYICY EIRVHALSES QGGCSSIRGD SKHKAPVSGP HITAITEKKE RLFISWTHIP FPEQRGCILH YRIYWKERDS TAQPELCEIQ YRRSQNSHPI SSLQPR. It is sometimes possible for the material contained within the vial of "Interleukin 12 Receptor Beta 2 (IL12Rb2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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