Goat anti-Human Interferon gamma Receptor Chain 1, neutralizing Polyclonal Antibody | anti-IFNGR1 antibody
Interferon gamma Receptor Chain 1, neutralizing (CDw119, IFNgR1) (Biotin)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Neutralization of Human IFN-g R1 Bioactivity:
The exact concentration of antibody required to neutralize rhIFN-g R1-mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response.
The ND50 is ~0.5-2ug/ml in the presence of 2ng/ml of rhIFN-g, using a confluent culture of HeLa cells.
ELISA: 0.5-1ug/ml. Direct ELISA. The detection limit for rhIFN-g R1 is ~0.3ng/well.
Immunohistochemistry: 15ug/ml. Detects IFN-g R1 in human PBMC (peripheral blood mononuclear cells)'s (Fluorescence immunocytochemistry.
Western Blot: 0.1-0.2ug/ml. The detection limit for is ~5ng/lane under non-reducing and reducing conditions.
NCBI and Uniprot Product Information
NCBI Description
This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. [provided by RefSeq]
Uniprot Description
IFNGR1: Receptor for interferon gamma. Two receptors bind one interferon gamma dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. Belongs to the type II cytokine receptor family.
Protein type: Receptor, cytokine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 6q23.3
Cellular Component: endoplasmic reticulum; integral to plasma membrane; dendrite; postsynaptic density; integral to membrane; plasma membrane; vesicle
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; interferon-gamma receptor activity; cytokine binding
Biological Process: response to virus; cytokine and chemokine mediated signaling pathway; signal transduction
Disease: Helicobacter Pylori Infection, Susceptibility To; Immunodeficiency 27b; Immunodeficiency 27a; Mycobacterium Tuberculosis, Susceptibility To; Hepatitis B Virus, Susceptibility To
Research Articles on IFNGR1
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Product Notes
The IFNGR1 ifngr1 (Catalog #AAA618765) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Interferon gamma Receptor Chain 1, neutralizing (CDw119, IFNgR1) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Interferon gamma Receptor Chain 1, neutralizing can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Neutralization. Suitable for use in ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry. Dilution: Neutralization of Human IFN-g R1 Bioactivity: The exact concentration of antibody required to neutralize rhIFN-g R1-mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 is ~0.5-2ug/ml in the presence of 2ng/ml of rhIFN-g, using a confluent culture of HeLa cells. ELISA: 0.5-1ug/ml. Direct ELISA. The detection limit for rhIFN-g R1 is ~0.3ng/well. Immunohistochemistry: 15ug/ml. Detects IFN-g R1 in human PBMC (peripheral blood mononuclear cells)'s (Fluorescence immunocytochemistry. Western Blot: 0.1-0.2ug/ml. The detection limit for is ~5ng/lane under non-reducing and reducing conditions. Researchers should empirically determine the suitability of the IFNGR1 ifngr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interferon gamma Receptor Chain 1, neutralizing, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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