SDS-Page
(SDS-PAGE)
Interferon Beta (IFNb) Active Protein | IFNb active protein
Active Interferon Beta (IFNb)
Gene Names
IFNB1; IFB; IFF; IFNB
Applications
Cell culture, Activity Assays
Purity
Greater than 97% by SDS-PAGE
Synonyms
Interferon Beta (IFNb); Active Interferon Beta (IFNb); IFNb/Interferon Beta; IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast; IFNb active protein
Host
E Coli
Purity/Purification
Greater than 97% by SDS-PAGE
Form/Format
Freeze-dried powder
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence
Met22~Asn187
Applicable Applications for IFNb active protein
Cell culture, Activity Assays
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Tag
N-terminal His Tag
Biological Activity
Active
Expression System
Prokaryotic expression
Organism Species
Human
Isoelectric Point
8.9
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
SDS-Page
(SDS-PAGE)
SDS-Page
(SDS-PAGE)
Sequence
Sequence
Related Product Information for IFNb active protein
Interferon Beta (IFNb) is belongs to type I interferons (IFNs) family which a largesubgroup of interferon proteins that help regulate the activity of the immunesystem. The IFNb proteins are produced in large quantities by fibroblasts. Theyhave antiviral activity that is involved mainly in innate immune response. Two types of IFNb have been described, IFNb1 (IFNB1) and IFNb3 (IFNB3). IFNb1 isused as a treatment for multiple sclerosis as it reduces the relapse rate. To testthe effect of IFNb on cell apoptosis, A549 cells were seeded into triplicate wellsof 96-well plates at a density of 2,000 cells/well and allowed to attach, replacedwith serum-free overnight, then the medium was replaced with 5% serum standard DMEM prior to the addition of various concentrations of recombinanthuman IFNb. After incubated for 48h, cells were observed by invertedmicroscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubatingthe plate for 1-4 hours at 37 degree C. Apoptosis of A549 cells after incubation withIFNb for 48h observed by inverted microscope was shown in Figure1. Cellviability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant IFNb for 48h.
Product Categories/Family for IFNb active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,294 Da
NCBI Official Full Name
interferon beta
NCBI Official Synonym Full Names
interferon, beta 1, fibroblast
NCBI Official Symbol
IFNB1
NCBI Official Synonym Symbols
IFB; IFF; IFNB
NCBI Protein Information
interferon beta; IFN-beta; fibroblast interferon
UniProt Protein Name
Interferon beta
Protein Family
UniProt Gene Name
IFNB1
UniProt Synonym Gene Names
IFB; IFNB; IFN-beta
UniProt Entry Name
IFNB_HUMAN
Uniprot Description
IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 9p21
Cellular Component: extracellular space; extracellular region
Molecular Function: interferon-alpha/beta receptor binding; cytokine activity
Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; cell surface receptor linked signal transduction; response to exogenous dsRNA; negative regulation of viral genome replication; B cell differentiation; defense response to bacterium; positive regulation of innate immune response; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein; B cell activation during immune response
Research Articles on IFNb
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Product Notes
The IFNb ifnb1 (Catalog #AAA2125333) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interferon Beta (IFNb) can be used in a range of immunoassay formats including, but not limited to, Cell culture, Activity Assays. Researchers should empirically determine the suitability of the IFNb ifnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met22~Asn1 87. It is sometimes possible for the material contained within the vial of "Interferon Beta (IFNb), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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