Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Intercellular Adhesion Molecule 4 Polyclonal Antibody | anti-ICAM4 antibody
Polyclonal Antibody to Intercellular Adhesion Molecule 4 (ICAM4)
Gene Names
Icam4; Cd242; ICAM-4; 1810015M19Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Intercellular Adhesion Molecule 4; Polyclonal Antibody; Polyclonal Antibody to Intercellular Adhesion Molecule 4 (ICAM4); anti-ICAM4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ICAM4. It has beenselected for its ability to recognize ICAM4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEFELRR QAC- QQEWMQSP PAPSVTSAPF WVRLNPELEA VPPGGSAWLN CSHNCPLPVH SSLRTQLRQG KIVNGSGWVS YQLLDVRAWN SKVRCVVTCA GETREATARI TAYKRPRSVI LEPPVLVGHK YTLRCYVTHV FPVGFLVVSL RRGGRVIYHE SLERFTGSDL ANVTLTYVMR AGLNDLWQPL
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEFELRR QAC- QQEWMQSP PAPSVTSAPF WVRLNPELEA VPPGGSAWLN CSHNCPLPVH SSLRTQLRQG KIVNGSGWVS YQLLDVRAWN SKVRCVVTCA GETREATARI TAYKRPRSVI LEPPVLVGHK YTLRCYVTHV FPVGFLVVSL RRGGRVIYHE SLERFTGSDL ANVTLTYVMR AGLNDLWQPL
Applicable Applications for anti-ICAM4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
ICAM4 (Gln23~Leu227)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant ICAM4 (Gln23~Leu227) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2056629
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Pancreas tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Pancreas tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,628 Da
NCBI Official Full Name
intercellular adhesion molecule 4
NCBI Official Synonym Full Names
intercellular adhesion molecule 4, Landsteiner-Wiener blood group
NCBI Official Symbol
Icam4
NCBI Official Synonym Symbols
Cd242; ICAM-4; 1810015M19Rik
NCBI Protein Information
intercellular adhesion molecule 4
UniProt Protein Name
Intercellular adhesion molecule 4
Protein Family
UniProt Gene Name
Icam4
UniProt Synonym Gene Names
ICAM-4
Uniprot Description
ICAM4: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins. Belongs to the immunoglobulin superfamily. ICAM family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9 A3|9 7.69 cM
Cellular Component: cytoplasm; extracellular region; extracellular space; integral component of membrane; integral component of plasma membrane; membrane; plasma membrane
Molecular Function: integrin binding
Biological Process: cell adhesion
Research Articles on ICAM4
Similar Products
Product Notes
The ICAM4 icam4 (Catalog #AAA2027503) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Intercellular Adhesion Molecule 4 (ICAM4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Intercellular Adhesion Molecule 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ICAM4 icam4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEFELRR QAC- QQEWMQSP PAPSVTSAPF WVRLNPELEA VPPGGSAWLN CSHNCPLPVH SSLRTQLRQG KIVNGSGWVS YQLLDVRAWN SKVRCVVTCA GETREATARI TAYKRPRSVI LEPPVLVGHK YTLRCYVTHV FPVGFLVVSL RRGGRVIYHE SLERFTGSDL ANVTLTYVMR AGLNDLWQPL. It is sometimes possible for the material contained within the vial of "Intercellular Adhesion Molecule 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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