Rabbit anti-Human Integrin-linked protein kinase Polyclonal Antibody | anti-ILK antibody

Rabbit anti-human Integrin-linked protein kinase polyclonal Antibody

Cat. Num. AAA7006239

• Gene Names: ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: Integrin-linked protein kinase; Polyclonal Antibody; Rabbit anti-human Integrin-linked protein kinase polyclonal Antibody; Integrin-linked protein kinase protein; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK; anti-ILK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Liquid
• Sequence Length: 452

• Applicable Applications for anti-ILK antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: IHC: 1:20-1:200
• Immunogen: Recombinant human Integrin-linked protein kinase protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7516 / sc-30713 / sc-13075
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: ILK antibody at 3ug/mlLane 1: Hela whole cell lysateLane 2: NIH/3T3 whole cell lysateLane 3: HL60 whole cell lysateLane 4: Mouse liver tissueLane 5: Mouse lung tissueLane 6: Rat liver tissueLane 7: Rat lung tissueLane 8: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 52,45,37 kDaObserved band size: 51,37 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded 293T cells using MBS7006239 at dilution of 1:100 Immunohistochemistry)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS7006239 at dilution of 1:20)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7006239 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-ILK antibody

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-ILK antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 62420873
• NCBI GeneID: 3611
• NCBI Accession #: NP_001014794.1
• NCBI GenBank Nucleotide #: NM_001014794.2
• UniProt Accession #: Q13418; P57043; Q68DZ3; B7Z1I0; B7Z418; D3DQU0
• Molecular Weight: 36,467 Da
• NCBI Official Full Name: integrin-linked protein kinase isoform 1
• NCBI Official Synonym Full Names: integrin linked kinase
• NCBI Official Symbol: ILK
• NCBI Official Synonym Symbols: P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• NCBI Protein Information: integrin-linked protein kinase
• UniProt Protein Name: Integrin-linked protein kinase
• Protein Family: Integrin-linked protein kinase
• UniProt Gene Name: ILK
• UniProt Synonym Gene Names: ILK1; ILK2
• UniProt Entry Name: ILK_HUMAN

NCBI Description

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; MLK family; ILK subfamily

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: cell junction; cell soma; costamere; cytoplasm; cytosol; dendritic shaft; extracellular matrix; focal adhesion; intercellular junction; lamellipodium; membrane; nucleoplasm; plasma membrane; protein complex; sarcomere; stress fiber; terminal button

Molecular Function: ATP binding; integrin binding; protein binding; protein kinase binding; protein serine/threonine kinase activity; SH3 domain binding; signal transducer activity

Biological Process: cell aging; cell cycle arrest; cell proliferation; cell-matrix adhesion; establishment and/or maintenance of epithelial cell polarity; fibril organization and biogenesis; integrin-mediated signaling pathway; myelin formation; myelination in the peripheral nervous system; negative regulation of neuron apoptosis; negative regulation of protein kinase activity; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; nerve development; neurite morphogenesis; peptidyl-serine phosphorylation; positive regulation of axon extension; positive regulation of BMP signaling pathway; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of dendrite morphogenesis; positive regulation of MAP kinase activity; positive regulation of myoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; protein heterooligomerization; protein kinase B signaling cascade; regulation of actin cytoskeleton organization and biogenesis; ureteric bud branching