Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide recombinant proteins and
antibodies to most
target proteins.
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '12105'
AND `pd`.`language_id` = 1
LIMIT 1
Query
Database
1.82 ms
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '12105' and pd.language_id = 1
Query
Database
1.33 ms
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '12105'
Database (4 total Queries, 4 of them unique across 2 Connections)
Time
Query String
2.45 ms
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '12105'
AND `pd`.`language_id` = 1
LIMIT 1
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '12105' and pd.language_id = 1
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '12105'
⇄⧉storage_stability => string (417) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
$value['storage_stability']
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (469) "Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cell...
$value['app_notes']
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.<br><b>Flow Cytometry: </b>Minimum Dilution: Neat; Maximum Dilution: 1/10; Application Note: <b>Membrane permeabilisation is required for this application. We recommends the use of Leucoperm<sup></sup> for this purpose.<br>The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.</b>
Application Data||Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p||AAA12105_APP10.jpg!!Application Data||Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.||AAA12105_APP9.jpg!!Application Data||Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp||AAA12105_APP8.jpg!!Application Data||Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.||AAA12105_APP7.jpg!!Application Data||Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. PLoS ONE 6(11): e28023.||AAA12105_APP6.jpg!!Application Data||Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 . following permeablisation with Leucoperm||AAA12105_APP5.gif!!Application Data||Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody||AAA12105_APP4.jpg!!Application Data||Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Medium power||AAA12105_APP3.jpg!!Application Data||Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Low power||AAA12105_APP2.jpg!!Application Data||Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 . following permeablisation with Leucoperm||AAA12105_APP.gif
Perservative Stabilisers||0.09% Sodium Azide<br>1% Bovine Serum Albumin<br>Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
⇄⧉etc_term2 => string (145) "Immunogen||Purified Concanavalin A acceptor glycoprotein from P815 cell line...
$value['etc_term2']
Immunogen||Purified Concanavalin A acceptor glycoprotein from P815 cell line<br>Buffer Solution||Phosphate buffered saline!!Target Species||Mouse
⇄⧉storage_stability => string (417) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
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Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (469) "Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cell...
$value->a['app_notes']
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.<br><b>Flow Cytometry: </b>Minimum Dilution: Neat; Maximum Dilution: 1/10; Application Note: <b>Membrane permeabilisation is required for this application. We recommends the use of Leucoperm<sup></sup> for this purpose.<br>The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.</b>
Application Data||Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p||AAA12105_APP10.jpg!!Application Data||Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.||AAA12105_APP9.jpg!!Application Data||Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp||AAA12105_APP8.jpg!!Application Data||Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.||AAA12105_APP7.jpg!!Application Data||Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. PLoS ONE 6(11): e28023.||AAA12105_APP6.jpg!!Application Data||Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 . following permeablisation with Leucoperm||AAA12105_APP5.gif!!Application Data||Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody||AAA12105_APP4.jpg!!Application Data||Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Medium power||AAA12105_APP3.jpg!!Application Data||Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Low power||AAA12105_APP2.jpg!!Application Data||Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 . following permeablisation with Leucoperm||AAA12105_APP.gif
Perservative Stabilisers||0.09% Sodium Azide<br>1% Bovine Serum Albumin<br>Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
⇄⧉etc_term2 => string (145) "Immunogen||Purified Concanavalin A acceptor glycoprotein from P815 cell line...
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Immunogen||Purified Concanavalin A acceptor glycoprotein from P815 cell line<br>Buffer Solution||Phosphate buffered saline!!Target Species||Mouse
⇄⧉storage_stability => string (417) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
$value->d['storage_stability']
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (469) "Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cell...
$value->d['app_notes']
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.<br><b>Flow Cytometry: </b>Minimum Dilution: Neat; Maximum Dilution: 1/10; Application Note: <b>Membrane permeabilisation is required for this application. We recommends the use of Leucoperm<sup></sup> for this purpose.<br>The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.</b>
Application Data||Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p||AAA12105_APP10.jpg!!Application Data||Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.||AAA12105_APP9.jpg!!Application Data||Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp||AAA12105_APP8.jpg!!Application Data||Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.||AAA12105_APP7.jpg!!Application Data||Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. PLoS ONE 6(11): e28023.||AAA12105_APP6.jpg!!Application Data||Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 . following permeablisation with Leucoperm||AAA12105_APP5.gif!!Application Data||Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody||AAA12105_APP4.jpg!!Application Data||Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Medium power||AAA12105_APP3.jpg!!Application Data||Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Low power||AAA12105_APP2.jpg!!Application Data||Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 . following permeablisation with Leucoperm||AAA12105_APP.gif
Perservative Stabilisers||0.09% Sodium Azide<br>1% Bovine Serum Albumin<br>Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
⇄⧉etc_term2 => string (145) "Immunogen||Purified Concanavalin A acceptor glycoprotein from P815 cell line...
$value->d['etc_term2']
Immunogen||Purified Concanavalin A acceptor glycoprotein from P815 cell line<br>Buffer Solution||Phosphate buffered saline!!Target Species||Mouse
⇄⧉products_description => string (445) "Could play a role in phagocytic activities of tissue macrophages, both in in...
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Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
WB (Western Blot)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.||AAA29179_WB7.jpg!!IHC (Immunohistchemistry)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.||AAA29179_IHC6.jpg!!IHC (Immunohistochemistry)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.||AAA29179_IHC5.jpg!!IHC (Immunohistochemistry-Paraffin)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.||AAA29179_IHC4.jpg!!IHC (Immunohistochemistry-Paraffin)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.||AAA29179_IHC3.jpg!!IHC (Immunohistochemistry-Paraffin)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.||AAA29179_IHC2.jpg!!WB (Western Blot)||Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.||AAA29179_WB.jpg
⇄⧉products_description => string (445) "Could play a role in phagocytic activities of tissue macrophages, both in in...
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Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
⇄⧉storage_stability => string (417) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
$value[2]['_source']['storage_stability']
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (469) "Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cell...
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Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.<br><b>Flow Cytometry: </b>Minimum Dilution: Neat; Maximum Dilution: 1/10; Application Note: <b>Membrane permeabilisation is required for this application. We recommends the use of Leucoperm<sup></sup> for this purpose.<br>The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.</b>
Application Data||Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p||AAA12105_APP10.jpg!!Application Data||Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.||AAA12105_APP9.jpg!!Application Data||Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp||AAA12105_APP8.jpg!!Application Data||Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.||AAA12105_APP7.jpg!!Application Data||Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. PLoS ONE 6(11): e28023.||AAA12105_APP6.jpg!!Application Data||Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 . following permeablisation with Leucoperm||AAA12105_APP5.gif!!Application Data||Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody||AAA12105_APP4.jpg!!Application Data||Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Medium power||AAA12105_APP3.jpg!!Application Data||Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Low power||AAA12105_APP2.jpg!!Application Data||Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 . following permeablisation with Leucoperm||AAA12105_APP.gif
Perservative Stabilisers||0.09% Sodium Azide<br>1% Bovine Serum Albumin<br>Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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Immunogen||Purified Concanavalin A acceptor glycoprotein from P815 cell line<br>Buffer Solution||Phosphate buffered saline!!Target Species||Mouse
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aaa12105 rat monoclonal igg2a fa 11 fitc purified igg conjugated to fluorescein isothiocyanate isomer 1 liquid flow cytometry fc facs * use 10ul of the suggested working dilution label 106 cells in 100ul minimum neat maximum 10 application note membrane permeabilisation is required for this mybiosource recommends leucoperm<sup>< sup> purpose region antibodies may bind non specifically expressing low affinity receptors be reduced by using seroblock fcr testing data staining permeabilised mouse peritoneal macrophages with anti cd68:alexa fluor 647 mbs215395a647 following permeablisation leucoperm aaa12105_td immunoperoxidase a lymph node cryosection cd68 antibody clone fa11 mbs215395 followed goat mbs235195 power aaa12105_td1 medium aaa12105_td2 western blot analysis expression on j774 mbs215403 igg:hrp as detection aaa12105_td3 alexa 488 mbs215395a488 aaa12105_td4 published customer image phenotype pancreatic identification macrophage subsets pancreas cx3cr1gfp + c56bl 6 mice single cell suspensions were gated fsc vs w and cd45+ histograms show receptor profile cx3cr1hilyve green line cx3cr1lolyve 1+ red b sorted spun onto glass slides stained wright's stain 1000x magnification c mrna chemokine chemokines vegfs levels examined qrt pcr duplicate or triplicate 7 group representative 2 4 separate experiments.from yin n zhang lal g xu j yan m et al 2011 lymphangiogenesis islet inflammation diabetes plos one e28023 aaa12105_td5 histology 9v gcstg null lung liver sections from 9 wk old row 3 processed h&e indicated large pale storage observed arrows immunostaining brown images captured zeiss microscope spot advance software scale bar was 40 um all distribution density immunostained both positive cd68+ lungs had significantly more than at wks age respectively present number per total 5 15 genotype results error bars are mean s.e p values student's t test.from barnes s y h liou setchell kdr 2014 ubiquitous transgene glucosylceramide synthesizing enzyme accelerates accumulation gaucher disease model 12 e116023 aaa12105_td6 spleen mbs220363 showing pulp aaa12105_td7 impact socs deficiency atherosclerotic plaque composition plaques triple ko showed an increased inos double content cd206 hardly detected contained slightly moma ly 6c after weeks hcd well d higher 6g 20 d:50 = animals group.from grothusen schuett hillmer lumpe grote k 2012 role suppressor cytokine signaling murine atherosclerosis e51608 aaa12105_td8 leukocyte infiltration cox 2+ mpo enzymatic activity panel statistically similar livers 24 post iri 6g+ neutrophil granulocyte also comparable mac e infiltrating reperfusion but indistinguishable no statistical differences mmp f could demonstrated a,b,e,f c,d,g,h leukocytes a,c,e,g b,d,f,h indicates aaa12105_td9 cd68:fitc antigen lamp4 gp110 scard1 macrosialin 33,844 da cd_antigen cd68_mouse 6753352 np_033983.1 p31996 nm_009853.1 o54688 o70321 q3s4a9 q5f2a8 q9dd15 perservative stabilisers 0.09 sodium azide bovine serum albumin preparation prepared chromatography protein tissue culture supernatant immunogen concanavalin acceptor glycoprotein p815 buffer solution phosphate buffered saline target species isomer1 label106 maximum10 fluor647 alexa488 c56bl6 triplicate7 representative2 from9 row3 was40 total5 model12 6g20 livers24
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Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
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Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.<br>Western Blot: This clone is suitable for use in western blotting applications.<br><b>Flow Cytometry: </b>Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: <b>Membrane permeabilisation is required for this application. We recommends the use of Leucoperm for this purpose.</b><br><b>Immunohistology - Paraffin: </b>Application Note: <b>This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase</b>
Application Data||Published customer image: Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A -D) H&E staining. E -H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats. PLoS ONE 9(10): e108533.||AAA12149_APP10.jpg!!Application Data||Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE . Permeabilised with Leucoperm (Fix & Perm)||AAA12149_APP9.gif!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Medium power||AAA12149_APP8.jpg!!Application Data||Published customer image: Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 um.From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats. PLoS ONE 9(12): e114694.||AAA12149_APP7.jpg!!Application Data||Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power||AAA12149_APP6.jpg!!Application Data||Published customer image: Western blotting of ED1 (Marker of activated microglia) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). Brains were treated with PBS or rAAV2/IL12 and then implanted with tumor on the last day of Week 2 post viral vector injection. The brains were used for Western blotting analysis of the expression of ED1, 34 kDa, and TRAIL, 104 kDa, from microglial cells on the last day of week 1, 2, and 3 following tumor implantation. The brain slices for Western blotting analysis are shown at the bottom. All brains were harvested on the last day of week 1, week 2, and week 3 post tumor implantation. The scale bars indicate 2 mm in brain sections.From: The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. Chiu TL, Wang MJ, Su CC. J Biomed Sci. 2012 Apr 22;19:45. doi: 10.1186/1423-0127-19-45.||AAA12149_APP5.jpg!!Application Data||Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm||AAA12149_APP4.gif!!Application Data||Published customer image: Immunohistochemistry of ED1 stain in brain sections treated with rAAV2/IL12 (A, B, C, D, n = 4), AAV2/GFP (E, F, n = 2), or PBS (G, H, n = 2), accompanied with tumor implantation; and that in the brain section treated with nothing (n = 1). Immunohistochemistry of brain sections was performed for ED1 on the last day of week 3 after tumor implantation. The brain sections were stained with hematoxylin for nuclei and ED1 for activated microglial cells. The 1st column shows the brain sections pictured before immunostaining; the 2nd column shows the brain sections pictured after staining; the 3rd to 6th columns show pictures taken at four quadrants (black squares in 2nd column) of the tumor adjacent to the normal tissue in the right hemisphere. ED1-positive cells show dark brown. The scale bars indicate 2 mm in 1st and 2nd columns and 100 um in 3rd-6th columns.From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. J Biomed Sci. 2012 Apr 22;19:45.||AAA12149_APP3.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power||AAA12149_APP2.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power||AAA12149_APP.jpg
Perservative Stabilisers||0.09% Sodium Azide<br>Preparation||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
aaa12149 mouse monoclonal igg1 ed1 purified igg liquid immunohistology frozen flow cytometry fc facs * immunofluorescence if immunoprecipitation ip paraffin* use 10ul of the suggested working dilution to label 106 cells in 100ul western blot this clone is suitable for blotting applications minimum 1 50 maximum 100 application note membrane permeabilisation required mybiosource recommends leucoperm purpose paraffin product requires protein digestion pre treatment sections e.g trypsin or pronase testing data staining rat lymph node cryosection with anti cd68 antibody red a and cd4 green b c merged image nuclei counter stained blue using dapi low power aaa12149_td high aaa12149_td1 published customer immunohistochemistry stain brain treated raav2 il12 d n = 4 aav2 gfp e f 2 pbs g h accompanied tumor implantation that section nothing was performed on last day week 3 after were hematoxylin activated microglial 1st column shows pictured before immunostaining 2nd 3rd 6th columns show pictures taken at four quadrants black squares adjacent normal tissue right hemisphere positive dark brown scale bars indicate mm um columns.from chiu tl wang mj su cc glioblastoma multiforme through activation microglia trail induced by mediated il 12 syngeneic model j biomed sci 2012 apr 22 19:45 aaa12149_td2 peritoneal macrophages alexa fluor 647 following aaa12149_td3 marker necrosis factor related apoptosis inducing ligand brains then implanted post viral vector injection used analysis expression 34 kda 104 from slices are shown bottom all harvested sections.from doi 10.1186 1423 0127 19 45 aaa12149_td4 immunoperoxidase followed horseradish peroxidase conjugated goat mbs235406 as detection reagent aaa12149_td5 immunohistochemical stains il1 optic nerve there cellular accumulation indicated crushed sites some positively suggesting recruitment bar um.from suzuki oku horie t morishita s tonari m et al 2014 changes aquaporin 9 crushing rats plos one e114694 aaa12149_td6 medium aaa12149_td7 cd68:rpe mbs216350 permeabilised fix perm aaa12149_td8 histological control hbot wounds wounding days 7 29 h&e cd34 i+j immunohistochemistry.from uk tong fijneman emg van neck jw hyperbaric oxygen therapy treat diabetes impaired wound healing 10 e108533 aaa12149_td9 macrosialin molecule antigen 72255507 np_001026808.1 nm_001031638.1 perservative stabilisers 0.09 sodium azide preparation prepared affinity chromatography culture supernatant buffer solution phosphate buffered saline target species label106 minimum1 maximum100 =4 f2 week3 apr22 fluor647 expression34 kda104 aquaporin9 days7 healing10
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Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (276) "Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cell...
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Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. <br><b>Flow Cytometry: </b>Maximum Dilution: Neat; Application Note: <b>Membrane permeabilisation is required for this application. We recommends the use of Leucoperm for this purpose.</b>
Application Data||Published customer image: Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A -D) H&E staining. E -H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats. PLoS ONE 9(10): e108533.||AAA12147_APP10.jpg!!Application Data||Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE . Permeabilised with Leucoperm (Fix & Perm)||AAA12147_APP9.gif!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Medium power||AAA12147_APP8.jpg!!Application Data||Published customer image: Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 um.From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats. PLoS ONE 9(12): e114694.||AAA12147_APP7.jpg!!Application Data||Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power||AAA12147_APP6.jpg!!Application Data||Published customer image: Western blotting of ED1 (Marker of activated microglia) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). Brains were treated with PBS or rAAV2/IL12 and then implanted with tumor on the last day of Week 2 post viral vector injection. The brains were used for Western blotting analysis of the expression of ED1, 34 kDa, and TRAIL, 104 kDa, from microglial cells on the last day of week 1, 2, and 3 following tumor implantation. The brain slices for Western blotting analysis are shown at the bottom. All brains were harvested on the last day of week 1, week 2, and week 3 post tumor implantation. The scale bars indicate 2 mm in brain sections.From: The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. Chiu TL, Wang MJ, Su CC. J Biomed Sci. 2012 Apr 22;19:45. doi: 10.1186/1423-0127-19-45.||AAA12147_APP5.jpg!!Application Data||Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm||AAA12147_APP4.gif!!Application Data||Published customer image: Immunohistochemistry of ED1 stain in brain sections treated with rAAV2/IL12 (A, B, C, D, n = 4), AAV2/GFP (E, F, n = 2), or PBS (G, H, n = 2), accompanied with tumor implantation; and that in the brain section treated with nothing (n = 1). Immunohistochemistry of brain sections was performed for ED1 on the last day of week 3 after tumor implantation. The brain sections were stained with hematoxylin for nuclei and ED1 for activated microglial cells. The 1st column shows the brain sections pictured before immunostaining; the 2nd column shows the brain sections pictured after staining; the 3rd to 6th columns show pictures taken at four quadrants (black squares in 2nd column) of the tumor adjacent to the normal tissue in the right hemisphere. ED1-positive cells show dark brown. The scale bars indicate 2 mm in 1st and 2nd columns and 100 um in 3rd-6th columns.From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. J Biomed Sci. 2012 Apr 22;19:45.||AAA12147_APP3.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power||AAA12147_APP2.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power||AAA12147_APP.jpg
Perservative Stabilisers||0.09% Sodium Azide<br>1% Bovine Serum Albumin<br>Preparation||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
aaa12147 mouse monoclonal igg1 ed1 biotin purified igg conjugated to liquid flow cytometry fc facs * use 10ul of the suggested working dilution label 106 cells in 100ul maximum neat application note membrane permeabilisation is required for this mybiosource recommends leucoperm purpose testing data immunofluorescence staining rat lymph node cryosection with anti cd68 antibody red a and cd4 green b c merged image nuclei counter stained blue using dapi low power aaa12147_td high aaa12147_td1 published customer immunohistochemistry stain brain sections treated raav2 il12 d n = 4 aav2 gfp e f 2 or pbs g h accompanied tumor implantation that section nothing 1 was performed on last day week 3 after were hematoxylin activated microglial 1st column shows pictured before immunostaining 2nd 3rd 6th columns show pictures taken at four quadrants black squares adjacent normal tissue right hemisphere positive dark brown scale bars indicate mm 100 um columns.from chiu tl wang mj su cc treatment glioblastoma multiforme through activation microglia trail induced by mediated il 12 syngeneic model j biomed sci 2012 apr 22 19:45 aaa12147_td2 peritoneal macrophages alexa fluor 647 following aaa12147_td3 western blotting marker necrosis factor related apoptosis inducing ligand brains then implanted post viral vector injection used analysis expression 34 kda 104 from slices are shown bottom all harvested sections.from doi 10.1186 1423 0127 19 45 aaa12147_td4 immunoperoxidase followed horseradish peroxidase goat mbs235406 as detection reagent aaa12147_td5 immunohistochemical stains il1 optic nerve there cellular accumulation indicated crushed sites some positively suggesting recruitment bar um.from suzuki oku horie t morishita s tonari m et al 2014 changes aquaporin 9 crushing rats plos one e114694 aaa12147_td6 medium aaa12147_td7 cd68:rpe mbs216350 permeabilised fix perm aaa12147_td8 histological control hbot wounds wounding days 7 29 h&e cd34 i+j immunohistochemistry.from uk tong fijneman emg van neck jw hyperbaric oxygen therapy treat diabetes impaired wound healing 10 e108533 aaa12147_td9 cd68:biotin macrosialin molecule antigen 72255507 np_001026808.1 nm_001031638.1 perservative stabilisers 0.09 sodium azide bovine serum albumin preparation prepared affinity chromatography protein culture supernatant buffer solution phosphate buffered saline target species label106 =4 f2 nothing1 week3 mm100 apr22 fluor647 expression34 kda104 aquaporin9 days7 healing10
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Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse CD68 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are
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aaa22421 mouse no cross reaction with other factors typical testing data standard curve for reference only aaa22421_sc elisa kit cd68 molecule gp110 lamp4 scard1 macrosialin antigen macrophage scavenger receptor class d member 1 34,654 da cd_antigen cd68_human 298665 aab25811.1 p34810 q53hr6 q53xi3 q96bi7 b4dvt4 153634 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 10 ng ml 0.156 sensitivity up to 0.05 intra precision member1 range10
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Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
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Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Recommended protocols are available Here<br><b>Flow Cytometry: </b>Minimum Dilution: Neat; Maximum Dilution: 1/10; Application Note: <b>Membrane permeabilisation is required for this application. We recommends the use of Leucoperm<sup></sup> for this purpose.<br>The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.</b>
Application Data||Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p||AAA12104_APP10.jpg!!Application Data||Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. 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Application Data||Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p||AAA12108_APP10.jpg!!Application Data||Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. 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Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.||AAA12108_APP7.jpg!!Application Data||Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. 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aaa12108 rat monoclonal igg2a fa 11 purified igg conjugated to r phycoerythrin rpe lyophilised flow cytometry fc facs * use 10ul of the suggested working dilution label 106 cells in 100ul maximum neat application note membrane permeabilisation is required for this mybiosource recommends leucoperm<sup>< sup> purpose region antibodies may bind non specifically expressing low affinity receptors be reduced by using seroblock fcr testing data staining permeabilised mouse peritoneal macrophages with anti cd68:alexa fluor 647 mbs215395a647 following permeablisation leucoperm aaa12108_td immunoperoxidase a lymph node cryosection cd68 antibody clone fa11 mbs215395 followed goat mbs235195 power aaa12108_td1 medium aaa12108_td2 western blot analysis expression on j774 mbs215403 igg:hrp as detection aaa12108_td3 alexa 488 mbs215395a488 aaa12108_td4 published customer image phenotype pancreatic identification macrophage subsets pancreas cx3cr1gfp + c56bl 6 mice single cell suspensions were gated fsc vs w and cd45+ histograms show receptor profile cx3cr1hilyve 1 green line cx3cr1lolyve 1+ red b sorted spun onto glass slides stained wright's stain 1000x magnification c mrna chemokine chemokines vegfs levels examined qrt pcr duplicate or triplicate 7 group representative 2 4 separate experiments.from yin n zhang lal g xu j yan m et al 2011 lymphangiogenesis islet inflammation diabetes plos one e28023 aaa12108_td5 histology 9v gcstg null lung liver sections from 9 wk old row 3 processed h&e indicated large pale storage observed arrows immunostaining brown images captured zeiss microscope spot advance software scale bar was 40 um all distribution density immunostained both positive cd68+ lungs had significantly more than at wks age respectively present number per total 5 15 genotype results error bars are mean s.e p values student's t test.from barnes s y h liou setchell kdr 2014 ubiquitous transgene glucosylceramide synthesizing enzyme accelerates accumulation gaucher disease model 12 e116023 aaa12108_td6 spleen mbs220363 showing pulp aaa12108_td7 impact socs deficiency atherosclerotic plaque composition plaques triple ko showed an increased inos double content cd206 hardly detected contained slightly moma ly 6c after weeks hcd well d higher 6g 20 d:50 = animals group.from grothusen schuett hillmer lumpe grote k 2012 role suppressor cytokine signaling murine atherosclerosis e51608 aaa12108_td8 leukocyte infiltration cox 2+ mpo enzymatic activity panel statistically similar livers 24 post iri 6g+ neutrophil granulocyte also comparable mac e infiltrating reperfusion but indistinguishable no statistical differences mmp f could demonstrated a,b,e,f c,d,g,h leukocytes a,c,e,g b,d,f,h indicates aaa12108_td9 cd68:rpe antigen lamp4 gp110 scard1 macrosialin 33,844 da cd_antigen cd68_mouse 6753352 np_033983.1 p31996 nm_009853.1 o54688 o70321 q3s4a9 q5f2a8 q9dd15 reconstitution reconstitute 1.0 ml distilled water perservative stabilisers 0.09 sodium azide bovine serum albumin sucrose preparation prepared chromatography protein tissue culture supernatant immunogen concanavalin acceptor glycoprotein p815 buffer solution phosphate buffered saline target species label106 fluor647 alexa488 c56bl6 cx3cr1hilyve1 triplicate7 representative2 from9 row3 was40 total5 model12 6g20 livers24 reconstitute1.0
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Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. <br><b>Flow Cytometry: </b>Minimum Dilution: Neat; Maximum Dilution: 1/10; Application Note: <b>Membrane permeabilisation is required for this application. We recommends the use of Leucoperm for this purpose.</b>
Application Data||Published customer image: Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A -D) H&E staining. E -H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats. PLoS ONE 9(10): e108533.||AAA12150_APP10.jpg!!Application Data||Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE. Permeabilised with Leucoperm (Fix & Perm)||AAA12150_APP9.gif!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Medium power||AAA12150_APP8.jpg!!Application Data||Published customer image: Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 um.From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats. PLoS ONE 9(12): e114694.||AAA12150_APP7.jpg!!Application Data||Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power||AAA12150_APP6.jpg!!Application Data||Published customer image: Western blotting of ED1 (Marker of activated microglia) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). Brains were treated with PBS or rAAV2/IL12 and then implanted with tumor on the last day of Week 2 post viral vector injection. The brains were used for Western blotting analysis of the expression of ED1, 34 kDa, and TRAIL, 104 kDa, from microglial cells on the last day of week 1, 2, and 3 following tumor implantation. The brain slices for Western blotting analysis are shown at the bottom. All brains were harvested on the last day of week 1, week 2, and week 3 post tumor implantation. The scale bars indicate 2 mm in brain sections.From: The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. Chiu TL, Wang MJ, Su CC. J Biomed Sci. 2012 Apr 22;19:45. doi: 10.1186/1423-0127-19-45.||AAA12150_APP5.jpg!!Application Data||Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm||AAA12150_APP4.gif!!Application Data||Published customer image: Immunohistochemistry of ED1 stain in brain sections treated with rAAV2/IL12 (A, B, C, D, n = 4), AAV2/GFP (E, F, n = 2), or PBS (G, H, n = 2), accompanied with tumor implantation; and that in the brain section treated with nothing (n = 1). Immunohistochemistry of brain sections was performed for ED1 on the last day of week 3 after tumor implantation. The brain sections were stained with hematoxylin for nuclei and ED1 for activated microglial cells. The 1st column shows the brain sections pictured before immunostaining; the 2nd column shows the brain sections pictured after staining; the 3rd to 6th columns show pictures taken at four quadrants (black squares in 2nd column) of the tumor adjacent to the normal tissue in the right hemisphere. ED1-positive cells show dark brown. The scale bars indicate 2 mm in 1st and 2nd columns and 100 um in 3rd-6th columns.From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. J Biomed Sci. 2012 Apr 22;19:45.||AAA12150_APP3.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power||AAA12150_APP2.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power||AAA12150_APP.jpg
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aaa12150 mouse monoclonal igg1 ed1 purified igg conjugated to r phycoerythrin rpe lyophilised flow cytometry fc facs * use 10ul of the suggested working dilution label 1x106 cells in 100ul minimum neat maximum 1 10 application note membrane permeabilisation is required for this mybiosource recommends leucoperm purpose testing data immunofluorescence staining rat lymph node cryosection with anti cd68 antibody red a and cd4 green b c merged image nuclei counter stained blue using dapi low power aaa12150_td high aaa12150_td1 published customer immunohistochemistry stain brain sections treated raav2 il12 d n = 4 aav2 gfp e f 2 or pbs g h accompanied tumor implantation that section nothing was performed on last day week 3 after were hematoxylin activated microglial 1st column shows pictured before immunostaining 2nd 3rd 6th columns show pictures taken at four quadrants black squares adjacent normal tissue right hemisphere positive dark brown scale bars indicate mm 100 um columns.from chiu tl wang mj su cc treatment glioblastoma multiforme through activation microglia trail induced by mediated il 12 syngeneic model j biomed sci 2012 apr 22 19:45 aaa12150_td2 peritoneal macrophages alexa fluor 647 following aaa12150_td3 western blotting marker necrosis factor related apoptosis inducing ligand brains then implanted post viral vector injection used analysis expression 34 kda 104 from slices are shown bottom all harvested sections.from doi 10.1186 1423 0127 19 45 aaa12150_td4 immunoperoxidase followed horseradish peroxidase goat mbs235406 as detection reagent aaa12150_td5 immunohistochemical stains il1 optic nerve there cellular accumulation indicated crushed sites some positively suggesting recruitment bar um.from suzuki oku horie t morishita s tonari m et al 2014 changes aquaporin 9 crushing rats plos one e114694 aaa12150_td6 medium aaa12150_td7 cd68:rpe permeabilised fix perm aaa12150_td8 histological control hbot wounds wounding days 7 29 h&e cd34 i+j immunohistochemistry.from uk tong fijneman emg van neck jw hyperbaric oxygen therapy treat diabetes impaired wound healing e108533 aaa12150_td9 macrosialin molecule antigen 72255507 np_001026808.1 nm_001031638.1 reconstitution reconstitute ml distilled water perservative stabilisers 0.09 sodium azide bovine serum albumin preparation prepared affinity chromatography protein culture supernatant buffer solution phosphate buffered saline target species maximum1 =4 f2 week3 mm100 apr22 fluor647 expression34 kda104 aquaporin9 days7
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Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)*
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Application Data||Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody||AAA12245_APP9.jpg!!Application Data||Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp||AAA12245_APP8.jpg!!Application Data||Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor<sup>R</sup> 647 . following permeablisation with Leucoperm||AAA12245_APP7.gif!!Application Data||Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: RPE . following permeablisation with Leucoperm||AAA12245_APP6.gif!!Application Data||Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor<sup>R</sup> 488 . following permeablisation with Leucoperm||AAA12245_APP5.gif!!Application Data||Staining of mouse peritoneal macrophages with Rat anti Mouse CD68 CD68 , following permeablisation with Leucoperm||AAA12245_APP4.gif!!Application Data||Staining of permeabilised mouse peritoneal macrophages cells with Rat anti Mouse CD68 visualised with F(ab')2 Goat anti Rat IgG:FITC (Mouse adsorbed)||AAA12245_APP3.gif!!Application Data||Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:FITC . following permeablisation with Leucoperm||AAA12245_APP2.gif!!Application Data||Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:Biotin .following permeablisation with Leucoperm||AAA12245_APP.gif
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Immunogen||Purified Concanavalin A acceptor glycoprotein from P815 cell line<br>Buffer Solution||Phosphate buffered saline!!Target Species||Mouse
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aaa12245 rat monoclonal igg2a fa 11 low endotoxin purified igg liquid immunohistology frozen flow cytometry fc facs * immunofluorescence if immunoprecipitation ip paraffin* western blot wb use 10ul of the suggested working dilution to label 1x106 cells in 100ul region antibodies may bind non specifically expressing affinity receptors this be reduced by using seroblock fcr minimum 1 50 maximum 100 application note membrane permeabilization is required for mybiosource recommends leucoperm purpose paraffin product require antigen retrieval heat treatment prior staining sections sodium citrate buffer ph 6.0 recommended see martin manso details has also been achieved without lu blotting reducing conditions testing data #1 mouse peritoneal macrophages with anti cd68:biotin mbs215398 following permeablisation aaa12245_td1 #2 cd68:fitc mbs215400 aaa12245_td2 #3 permeabilised cd68 mbs215395 visualised f ab' 2 goat igg:fitc adsorbed mbs235148 aaa12245_td3 #4 mbs215403 aaa12245_td4 #5 alexa fluorr 488 aaa12245_td5 #6 rpe mbs215405 aaa12245_td6 #7 cd68:alexa 647 aaa12245_td7 #8 spleen cryosection followed igg:hrp mbs220363 showing red pulp aaa12245_td8 #9 analysis expression on j774 mbs235195 as a detection antibody aaa12245_td9 cd68:endotoxin lamp4 gp110 scard1 macrosialin 33,844 da cd_antigen cd68_mouse 6753352 np_033983.1 p31996 nm_009853.1 o54688 o70321 q3s4a9 q5f2a8 q9dd15 preparation prepared chromatography protein g from tissue culture supernatant immunogen concanavalin acceptor glycoprotein p815 cell line solution phosphate buffered saline target species fa11 minimum1 maximum100 ph6.0 ab'2 fluorr488 cd68:alexa647
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Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.<br><b>Flow Cytometry: </b>Maximum Dilution: Neat; Application Note: <b>Membrane permeabilisation is required for this application. We recommends the use of Leucoperm<sup></sup> for this purpose.<br>The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.</b>
Application Data||Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p||AAA12109_APP10.jpg!!Application Data||Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.||AAA12109_APP9.jpg!!Application Data||Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp||AAA12109_APP8.jpg!!Application Data||Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.||AAA12109_APP7.jpg!!Application Data||Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. PLoS ONE 6(11): e28023.||AAA12109_APP6.jpg!!Application Data||Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 . following permeablisation with Leucoperm||AAA12109_APP5.gif!!Application Data||Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody||AAA12109_APP4.jpg!!Application Data||Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Medium power||AAA12109_APP3.jpg!!Application Data||Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Low power||AAA12109_APP2.jpg!!Application Data||Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 . following permeablisation with Leucoperm||AAA12109_APP.gif
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Immunogen||Purified Concanavalin A acceptor glycoprotein from P815 cell line<br>Buffer Solution||Phosphate buffered saline!!Target Species||Mouse
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aaa12109 rat monoclonal igg2a fa 11 purified igg conjugated to r phycoerythrin rpe lyophilised flow cytometry fc facs * use 10ul of the suggested working dilution label 106 cells in 100ul maximum neat application note membrane permeabilisation is required for this mybiosource recommends leucoperm<sup>< sup> purpose region antibodies may bind non specifically expressing low affinity receptors be reduced by using seroblock fcr testing data staining permeabilised mouse peritoneal macrophages with anti cd68:alexa fluor 647 mbs215395a647 following permeablisation leucoperm aaa12109_td immunoperoxidase a lymph node cryosection cd68 antibody clone fa11 mbs215395 followed goat mbs235195 power aaa12109_td1 medium aaa12109_td2 western blot analysis expression on j774 mbs215403 igg:hrp as detection aaa12109_td3 alexa 488 mbs215395a488 aaa12109_td4 published customer image phenotype pancreatic identification macrophage subsets pancreas cx3cr1gfp + c56bl 6 mice single cell suspensions were gated fsc vs w and cd45+ histograms show receptor profile cx3cr1hilyve 1 green line cx3cr1lolyve 1+ red b sorted spun onto glass slides stained wright's stain 1000x magnification c mrna chemokine chemokines vegfs levels examined qrt pcr duplicate or triplicate 7 group representative 2 4 separate experiments.from yin n zhang lal g xu j yan m et al 2011 lymphangiogenesis islet inflammation diabetes plos one e28023 aaa12109_td5 histology 9v gcstg null lung liver sections from 9 wk old row 3 processed h&e indicated large pale storage observed arrows immunostaining brown images captured zeiss microscope spot advance software scale bar was 40 um all distribution density immunostained both positive cd68+ lungs had significantly more than at wks age respectively present number per total 5 15 genotype results error bars are mean s.e p values student's t test.from barnes s y h liou setchell kdr 2014 ubiquitous transgene glucosylceramide synthesizing enzyme accelerates accumulation gaucher disease model 12 e116023 aaa12109_td6 spleen mbs220363 showing pulp aaa12109_td7 impact socs deficiency atherosclerotic plaque composition plaques triple ko showed an increased inos double content cd206 hardly detected contained slightly moma ly 6c after weeks hcd well d higher 6g 20 d:50 = animals group.from grothusen schuett hillmer lumpe grote k 2012 role suppressor cytokine signaling murine atherosclerosis e51608 aaa12109_td8 leukocyte infiltration cox 2+ mpo enzymatic activity panel statistically similar livers 24 post iri 6g+ neutrophil granulocyte also comparable mac e infiltrating reperfusion but indistinguishable no statistical differences mmp f could demonstrated a,b,e,f c,d,g,h leukocytes a,c,e,g b,d,f,h indicates aaa12109_td9 cd68:rpe antigen lamp4 gp110 scard1 macrosialin 33,844 da cd_antigen cd68_mouse 6753352 np_033983.1 p31996 nm_009853.1 o54688 o70321 q3s4a9 q5f2a8 q9dd15 reconstitution reconstitute 0.25 ml disilled water perservative stabilisers 0.09 sodium azide bovine serum albumin sucrose preparation prepared chromatography protein tissue culture supernatant immunogen concanavalin acceptor glycoprotein p815 buffer solution phosphate buffered saline target species label106 fluor647 alexa488 c56bl6 cx3cr1hilyve1 triplicate7 representative2 from9 row3 was40 total5 model12 6g20 livers24
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Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)*
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Application Data||Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p||AAA12102_APP10.jpg!!Application Data||Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.||AAA12102_APP9.jpg!!Application Data||Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp||AAA12102_APP8.jpg!!Application Data||Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). 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aaa12102 rat monoclonal igg2a fa 11 purified igg liquid immunohistology frozen flow cytometry fc facs * immunofluorescence if immunoprecipitation ip paraffin* western blot wb use 10ul of the suggested working dilution to label 106 cells in 100ul minimum 1 50 maximum 100 application note membrane permeabilisation is required for this mybiosource recommends leucoperm paraffin product may require antigen retrieval using heat treatment prior staining sections either sodium citrate buffer or tris edta be used purpose see martin manso details has also been achieved without lu blotting non reducing conditions recommended testing data permeabilised mouse peritoneal macrophages with anti cd68:alexa fluor 647 aaa12102a647 following permeablisation aaa12102_td immunoperoxidase a lymph node cryosection cd68 antibody clone fa11 followed by goat mbs235195 low power aaa12102_td1 medium aaa12102_td2 analysis expression on j774 mbs215403 igg:hrp as detection aaa12102_td3 alexa 488 aaa12102a488 aaa12102_td4 published customer image phenotype pancreatic identification macrophage subsets pancreas cx3cr1gfp + c56bl 6 mice single cell suspensions were gated fsc vs w and cd45+ histograms show receptor profile cx3cr1hilyve green line cx3cr1lolyve 1+ red b sorted spun onto glass slides stained wright's stain 1000x magnification c mrna chemokine receptors chemokines vegfs levels examined qrt pcr duplicate triplicate 7 group representative 2 4 separate experiments.from yin n zhang lal g xu j yan m et al 2011 lymphangiogenesis islet inflammation diabetes plos one e28023 aaa12102_td5 histology 9v gcstg null lung liver from 9 wk old row 3 processed h&e indicated large pale storage observed arrows immunostaining brown images captured zeiss microscope spot advance software scale bar was 40 um all distribution density immunostained both positive cd68+ lungs had significantly more than at wks age respectively present number per total 5 15 genotype results error bars are mean s.e p values student's t test.from barnes s y h liou setchell kdr 2014 ubiquitous transgene glucosylceramide synthesizing enzyme accelerates accumulation gaucher disease model 12 e116023 aaa12102_td6 spleen mbs220363 showing pulp aaa12102_td7 impact socs deficiency atherosclerotic plaque composition plaques triple ko showed an increased inos double content cd206 hardly detected contained slightly moma ly 6c after weeks hcd well d higher 6g 20 d:50 = animals group.from grothusen schuett hillmer lumpe grote k 2012 role suppressor cytokine signaling murine atherosclerosis e51608 aaa12102_td8 leukocyte infiltration cox 2+ mpo enzymatic activity panel statistically similar livers 24 post iri 6g+ neutrophil granulocyte comparable mac e infiltrating reduced reperfusion but indistinguishable no statistical differences mmp f could demonstrated a,b,e,f c,d,g,h leukocytes a,c,e,g b,d,f,h indicates aaa12102_td9 lamp4 gp110 scard1 macrosialin 33,844 da cd_antigen cd68_mouse 6753352 np_033983.1 p31996 nm_009853.1 o54688 o70321 q3s4a9 q5f2a8 q9dd15 perservative stabilisers 0.09 azide preparation prepared affinity chromatography protein tissue culture supernatant immunogen concanavalin acceptor glycoprotein p815 solution phosphate buffered saline target species label106 minimum1 maximum100 fluor647 alexa488 c56bl6 triplicate7 representative2 from9 row3 was40 total5 model12 6g20 livers24
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Application Data||Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p||AAA12106_APP10.jpg!!Application Data||Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.||AAA12106_APP9.jpg!!Application Data||Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp||AAA12106_APP8.jpg!!Application Data||Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.||AAA12106_APP7.jpg!!Application Data||Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. 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aaa12106 rat monoclonal igg2a fa 11 fitc purified igg conjugated to fluorescein isothiocyanate isomer 1 liquid flow cytometry fc facs * use 10ul of the suggested working dilution label 106 cells in 100ul minimum neat maximum 10 application note membrane permeabilisation is required for this mybiosource recommends leucoperm<sup>< sup> purpose region antibodies may bind non specifically expressing low affinity receptors be reduced by using seroblock fcr testing data staining permeabilised mouse peritoneal macrophages with anti cd68:alexa fluor 647 mbs215395a647 following permeablisation leucoperm aaa12106_td immunoperoxidase a lymph node cryosection cd68 antibody clone fa11 mbs215395 followed goat mbs235195 power aaa12106_td1 medium aaa12106_td2 western blot analysis expression on j774 mbs215403 igg:hrp as detection aaa12106_td3 alexa 488 mbs215395a488 aaa12106_td4 published customer image phenotype pancreatic identification macrophage subsets pancreas cx3cr1gfp + c56bl 6 mice single cell suspensions were gated fsc vs w and cd45+ histograms show receptor profile cx3cr1hilyve green line cx3cr1lolyve 1+ red b sorted spun onto glass slides stained wright's stain 1000x magnification c mrna chemokine chemokines vegfs levels examined qrt pcr duplicate or triplicate 7 group representative 2 4 separate experiments.from yin n zhang lal g xu j yan m et al 2011 lymphangiogenesis islet inflammation diabetes plos one e28023 aaa12106_td5 histology 9v gcstg null lung liver sections from 9 wk old row 3 processed h&e indicated large pale storage observed arrows immunostaining brown images captured zeiss microscope spot advance software scale bar was 40 um all distribution density immunostained both positive cd68+ lungs had significantly more than at wks age respectively present number per total 5 15 genotype results error bars are mean s.e p values student's t test.from barnes s y h liou setchell kdr 2014 ubiquitous transgene glucosylceramide synthesizing enzyme accelerates accumulation gaucher disease model 12 e116023 aaa12106_td6 spleen mbs220363 showing pulp aaa12106_td7 impact socs deficiency atherosclerotic plaque composition plaques triple ko showed an increased inos double content cd206 hardly detected contained slightly moma ly 6c after weeks hcd well d higher 6g 20 d:50 = animals group.from grothusen schuett hillmer lumpe grote k 2012 role suppressor cytokine signaling murine atherosclerosis e51608 aaa12106_td8 leukocyte infiltration cox 2+ mpo enzymatic activity panel statistically similar livers 24 post iri 6g+ neutrophil granulocyte also comparable mac e infiltrating reperfusion but indistinguishable no statistical differences mmp f could demonstrated a,b,e,f c,d,g,h leukocytes a,c,e,g b,d,f,h indicates aaa12106_td9 cd68:fitc antigen lamp4 gp110 scard1 macrosialin 33,844 da cd_antigen cd68_mouse 6753352 np_033983.1 p31996 nm_009853.1 o54688 o70321 q3s4a9 q5f2a8 q9dd15 perservative stabilisers 0.09 sodium azide bovine serum albumin preparation prepared chromatography protein tissue culture supernatant immunogen concanavalin acceptor glycoprotein p815 buffer solution phosphate buffered saline target species isomer1 label106 maximum10 fluor647 alexa488 c56bl6 triplicate7 representative2 from9 row3 was40 total5 model12 6g20 livers24
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Application Data||Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p||AAA12103_APP10.jpg!!Application Data||Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.||AAA12103_APP9.jpg!!Application Data||Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp||AAA12103_APP8.jpg!!Application Data||Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). 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Application Data||Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p||AAA12110_APP10.jpg!!Application Data||Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.||AAA12110_APP9.jpg!!Application Data||Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp||AAA12110_APP8.jpg!!Application Data||Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.||AAA12110_APP7.jpg!!Application Data||Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. 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Immunohistology Frozen, Flow cytometry (FC/FACS)<sup><b>1</b></sup>, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin<sup><b>2</b></sup>, Western Blot (WB)<sup><b>3</b></sup>
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Application Data||Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p||AAA12107_APP10.jpg!!Application Data||Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. 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Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.||AAA12107_APP7.jpg!!Application Data||Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. 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⇄⧉products_description => string (1415) "<b>Specificity:</b> Rat anti Mouse CD6S antibody, clone FA-11 recognizes mou...
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<b>Specificity:</b> Rat anti Mouse CD6S antibody, clone FA-11 recognizes mouse macrosialin , a heavily glycosylated transmembrane protein and murine homolog of human CoG8, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain. C068 is considered a pan macrophage marker, predominantly expressed on the intracellular Iysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes.C068 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used in cancer studies to demonstrate the presence/localization of macrophages. Rat anti mouse Co68 antibody, clone FA-11 , has been used in many mouse models for the identification of C068 in immunohistochemical stud ies, using both frozen and paraffinembedded tissues (Masaki et al. 2003) and (Devey et al. 2009) . Rat anti mouse Co68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular C068, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
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⇄⧉products_related_diseases => string (223) "Inflammation||1681!!Nervous System Diseases||1021!!Cardiovascular Diseases||...
⇄⧉search_terms => string (3345) "aaa12107 rat monoclonal igg2a fa 11 purified igg liquid immunohistology froz...
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aaa12107 rat monoclonal igg2a fa 11 purified igg liquid immunohistology frozen flow cytometry fc facs 1 immunofluorescence if immunoprecipitation ip paraffin 2 western blot wb 3 use 10ul of the suggested working dilution to label 106 cells in 100ul 50 100 membrane permeabilisation is required for this application mybiosource recommends leucoperm product may require antigen retrieval using heat treatment prior staining sections.either sodium citrate buffer or tris edta be used purpose see martin manso details has also been achieved without lu non reducing conditions recommended testing data permeabilised mouse peritoneal macrophages with anti cd68:alexa fluor 647 mbs215395a647 following permeablisation aaa12107_td immunoperoxidase a lymph node cryosection cd68 antibody clone fa11 mbs215395 followed by goat mbs235195 low power aaa12107_ip medium aaa12107_ip1 analysis expression on j774 igg:hrp as detection aaa12107_wb1 alexa 488 mbs215395a488 aaa12107_td4 published customer image phenotype pancreatic identification macrophage subsets pancreas cx3cr1gfp + c56bl 6 mice single cell suspensions were gated fsc vs w and cd45+ histograms show receptor profile cx3cr1hilyve green line cx3cr1lolyve 1+ red b sorted spun onto glass slides stained wright's stain 1000x magnification c mrna chemokine receptors chemokines vegfs levels examined qrt pcr duplicate triplicate 7 group representative 4 separate experiments.from yin n zhang lal g xu j yan m et al 2011 lymphangiogenesis islet inflammation diabetes plos one e28023 aaa12107_td5 histology 9v gcstg null lung liver sections from 9 wk old row processed h&e indicated large pale storage observed arrows immunostaining brown images captured zeiss microscope spot advance software scale bar was 40 um all distribution density immunostained both positive cd68+ lungs had significantly more than at wks age respectively present number per total 5 15 genotype results error bars are mean s.e p values student's t test.from barnes s y h liou setchell kdr 2014 ubiquitous transgene glucosylceramide synthesizing enzyme accelerates accumulation gaucher disease model 12 e116023 aaa12107_td10 spleen mbs220363 showing pulp aaa12107_ip2 impact socs deficiency atherosclerotic plaque composition plaques triple ko showed an increased inos double content cd206 hardly detected contained slightly moma ly 6c after weeks hcd well d higher 6g 20 d:50 = animals group.from grothusen schuett hillmer lumpe grote k 2012 role suppressor cytokine signaling murine atherosclerosis e51608 aaa12107_td8 leukocyte infiltration cox 2+ mpo enzymatic activity panel statistically similar livers 24 post iri 6g+ neutrophil granulocyte comparable mac e infiltrating reduced reperfusion but indistinguishable no statistical differences mmp f could demonstrated a,b,e,f c,d,g,h leukocytes a,c,e,g b,d,f,h * indicates aaa12107_td9 lamp4 gp110 scard1 macrosialin cd_antigen cd68_mouse 6753352 np_033983.1 p31996 nm_009853.1 o54688 o70321 q3s4a9 q5f2a8 q9dd15 perservative stabilisers 0.09 azide preparation prepared affinity chromatography protein tissue culture supernatant immunogen concanavalin acceptor glycoprotein p815 solution phosphate buffered saline target species preperation facs1 paraffin2 wb3 label106 100ul50 fluor647 alexa488 c56bl6 triplicate7 representative4 from9 was40 total5 model12 6g20 livers24
⇄⧉storage_stability => string (350) "Store at 4 degree C or at -20 degree C if preferred. This product should be ...
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Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Radioimmunoassays (RIA), Western Blot (WB)
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Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. <br><b>Flow Cytometry: </b>Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: <b> Membrane permeabilisation is required for this application. We recommends the use of Leucoperm for this purpose.</b><br><b>Immunohistology - Paraffin: </b>Maximum Dilution: 1/100; Application Note: <b> This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase</b>
Application Data||Published customer image: Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A -D) H&E staining. E -H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats. PLoS ONE 9(10): e108533.||AAA12151_APP10.jpg!!Application Data||Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE . Permeabilised with Leucoperm (Fix & Perm)||AAA12151_APP9.gif!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Medium power||AAA12151_APP8.jpg!!Application Data||Published customer image: Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 um.From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats. PLoS ONE 9(12): e114694.||AAA12151_APP7.jpg!!Application Data||Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power||AAA12151_APP6.jpg!!Application Data||Published customer image: Western blotting of ED1 (Marker of activated microglia) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). Brains were treated with PBS or rAAV2/IL12 and then implanted with tumor on the last day of Week 2 post viral vector injection. The brains were used for Western blotting analysis of the expression of ED1, 34 kDa, and TRAIL, 104 kDa, from microglial cells on the last day of week 1, 2, and 3 following tumor implantation. The brain slices for Western blotting analysis are shown at the bottom. All brains were harvested on the last day of week 1, week 2, and week 3 post tumor implantation. The scale bars indicate 2 mm in brain sections.From: The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. Chiu TL, Wang MJ, Su CC. J Biomed Sci. 2012 Apr 22;19:45. doi: 10.1186/1423-0127-19-45.||AAA12151_APP5.jpg!!Application Data||Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm||AAA12151_APP4.gif!!Application Data||Published customer image: Immunohistochemistry of ED1 stain in brain sections treated with rAAV2/IL12 (A, B, C, D, n = 4), AAV2/GFP (E, F, n = 2), or PBS (G, H, n = 2), accompanied with tumor implantation; and that in the brain section treated with nothing (n = 1). Immunohistochemistry of brain sections was performed for ED1 on the last day of week 3 after tumor implantation. The brain sections were stained with hematoxylin for nuclei and ED1 for activated microglial cells. The 1st column shows the brain sections pictured before immunostaining; the 2nd column shows the brain sections pictured after staining; the 3rd to 6th columns show pictures taken at four quadrants (black squares in 2nd column) of the tumor adjacent to the normal tissue in the right hemisphere. ED1-positive cells show dark brown. The scale bars indicate 2 mm in 1st and 2nd columns and 100 um in 3rd-6th columns.From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. J Biomed Sci. 2012 Apr 22;19:45.||AAA12151_APP3.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power||AAA12151_APP2.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power||AAA12151_APP.jpg
Perservative Stabilisers||0.09% sodium azide.<br>Preparation||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
aaa12151 mouse monoclonal igg1 ed1 purified igg liquid immunohistology frozen flow cytometry fc facs * immunofluorescence if immunoprecipitation ip paraffin* radioimmunoassays ria western blot wb use 10ul of the suggested working dilution to label 1x106 cells in 100ul minimum 1 50 maximum 100 application note membrane permeabilisation is required for this mybiosource recommends leucoperm purpose paraffin product requires protein digestion pre treatment sections e.g trypsin or pronase testing data staining rat lymph node cryosection with anti cd68 antibody red a and cd4 green b c merged image nuclei counter stained blue using dapi low power aaa12151_td high aaa12151_td1 published customer immunohistochemistry stain brain treated raav2 il12 d n = 4 aav2 gfp e f 2 pbs g h accompanied tumor implantation that section nothing was performed on last day week 3 after were hematoxylin activated microglial 1st column shows pictured before immunostaining 2nd 3rd 6th columns show pictures taken at four quadrants black squares adjacent normal tissue right hemisphere positive dark brown scale bars indicate mm um columns.from chiu tl wang mj su cc glioblastoma multiforme through activation microglia trail induced by mediated il 12 syngeneic model j biomed sci 2012 apr 22 19:45 aaa12151_td2 peritoneal macrophages alexa fluor 647 following aaa12151_td3 blotting marker necrosis factor related apoptosis inducing ligand brains then implanted post viral vector injection used analysis expression 34 kda 104 from slices are shown bottom all harvested sections.from doi 10.1186 1423 0127 19 45 aaa12151_td4 immunoperoxidase followed horseradish peroxidase conjugated goat mbs235406 as detection reagent aaa12151_td5 immunohistochemical stains il1 optic nerve there cellular accumulation indicated crushed sites some positively suggesting recruitment bar um.from suzuki oku horie t morishita s tonari m et al 2014 changes aquaporin 9 crushing rats plos one e114694 aaa12151_td6 medium aaa12151_td7 cd68:rpe mbs216350 permeabilised fix perm aaa12151_td8 histological control hbot wounds wounding days 7 29 h&e cd34 i+j immunohistochemistry.from uk tong fijneman emg van neck jw hyperbaric oxygen therapy treat diabetes impaired wound healing 10 e108533 aaa12151_td9 macrosialin molecule antigen 72255507 np_001026808.1 nm_001031638.1 perservative stabilisers 0.09 sodium azide preparation prepared affinity chromatography culture supernatant buffer solution phosphate buffered saline target species minimum1 maximum100 =4 f2 week3 apr22 fluor647 expression34 kda104 aquaporin9 days7 healing10
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Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.<br>Shelf Life: 18 months from date of despatch.
⇄⧉app_notes => string (276) "Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cell...
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Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. <br><b>Flow Cytometry: </b>Maximum Dilution: Neat; Application Note: <b>Membrane permeabilisation is required for this application. We recommends the use of Leucoperm for this purpose.</b>
Application Data||Published customer image: Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A -D) H&E staining. E -H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats. PLoS ONE 9(10): e108533.||AAA12148_APP10.jpg!!Application Data||Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE . Permeabilised with Leucoperm (Fix & Perm)||AAA12148_APP9.gif!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Medium power||AAA12148_APP8.jpg!!Application Data||Published customer image: Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 um.From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats. PLoS ONE 9(12): e114694.||AAA12148_APP7.jpg!!Application Data||Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power||AAA12148_APP6.jpg!!Application Data||Published customer image: Western blotting of ED1 (Marker of activated microglia) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). Brains were treated with PBS or rAAV2/IL12 and then implanted with tumor on the last day of Week 2 post viral vector injection. The brains were used for Western blotting analysis of the expression of ED1, 34 kDa, and TRAIL, 104 kDa, from microglial cells on the last day of week 1, 2, and 3 following tumor implantation. The brain slices for Western blotting analysis are shown at the bottom. All brains were harvested on the last day of week 1, week 2, and week 3 post tumor implantation. The scale bars indicate 2 mm in brain sections.From: The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. Chiu TL, Wang MJ, Su CC. J Biomed Sci. 2012 Apr 22;19:45. doi: 10.1186/1423-0127-19-45.||AAA12148_APP5.jpg!!Application Data||Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm||AAA12148_APP4.gif!!Application Data||Published customer image: Immunohistochemistry of ED1 stain in brain sections treated with rAAV2/IL12 (A, B, C, D, n = 4), AAV2/GFP (E, F, n = 2), or PBS (G, H, n = 2), accompanied with tumor implantation; and that in the brain section treated with nothing (n = 1). Immunohistochemistry of brain sections was performed for ED1 on the last day of week 3 after tumor implantation. The brain sections were stained with hematoxylin for nuclei and ED1 for activated microglial cells. The 1st column shows the brain sections pictured before immunostaining; the 2nd column shows the brain sections pictured after staining; the 3rd to 6th columns show pictures taken at four quadrants (black squares in 2nd column) of the tumor adjacent to the normal tissue in the right hemisphere. ED1-positive cells show dark brown. The scale bars indicate 2 mm in 1st and 2nd columns and 100 um in 3rd-6th columns.From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. J Biomed Sci. 2012 Apr 22;19:45.||AAA12148_APP3.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power||AAA12148_APP2.jpg!!Application Data||Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power||AAA12148_APP.jpg
Perservative Stabilisers||0.09% Sodium Azide<br>1% Bovine Serum Albumin<br>Preparation||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
aaa12148 mouse monoclonal igg1 ed1 fitc purified igg conjugated to fluorescein isothiocyanate isomer 1 liquid flow cytometry fc facs * use 10ul of the suggested working dilution label 106 cells in 100ul maximum neat application note membrane permeabilisation is required for this mybiosource recommends leucoperm purpose testing data immunofluorescence staining rat lymph node cryosection with anti cd68 antibody red a and cd4 green b c merged image nuclei counter stained blue using dapi low power aaa12148_td high aaa12148_td1 published customer immunohistochemistry stain brain sections treated raav2 il12 d n = 4 aav2 gfp e f 2 or pbs g h accompanied tumor implantation that section nothing was performed on last day week 3 after were hematoxylin activated microglial 1st column shows pictured before immunostaining 2nd 3rd 6th columns show pictures taken at four quadrants black squares adjacent normal tissue right hemisphere positive dark brown scale bars indicate mm 100 um columns.from chiu tl wang mj su cc treatment glioblastoma multiforme through activation microglia trail induced by mediated il 12 syngeneic model j biomed sci 2012 apr 22 19:45 aaa12148_td2 peritoneal macrophages alexa fluor 647 following aaa12148_td3 western blotting marker necrosis factor related apoptosis inducing ligand brains then implanted post viral vector injection used analysis expression 34 kda 104 from slices are shown bottom all harvested sections.from doi 10.1186 1423 0127 19 45 aaa12148_td4 immunoperoxidase followed horseradish peroxidase goat mbs235406 as detection reagent aaa12148_td5 immunohistochemical stains il1 optic nerve there cellular accumulation indicated crushed sites some positively suggesting recruitment bar um.from suzuki oku horie t morishita s tonari m et al 2014 changes aquaporin 9 crushing rats plos one e114694 aaa12148_td6 medium aaa12148_td7 cd68:rpe mbs216350 permeabilised fix perm aaa12148_td8 histological control hbot wounds wounding days 7 29 h&e cd34 i+j immunohistochemistry.from uk tong fijneman emg van neck jw hyperbaric oxygen therapy treat diabetes impaired wound healing 10 e108533 aaa12148_td9 cd68:fitc macrosialin molecule antigen 72255507 np_001026808.1 nm_001031638.1 perservative stabilisers 0.09 sodium azide bovine serum albumin preparation prepared affinity chromatography protein culture supernatant buffer solution phosphate buffered saline target species isomer1 label106 =4 f2 week3 mm100 apr22 fluor647 expression34 kda104 aquaporin9 days7 healing10
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This assay has high sensitivity and excellent specificity for detection of rat CD68. No significant cross-reactivity or interference between rat CD68 and analogues was observed.
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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Rat Cluster of Differentiation 68; CD68 ELISA kit; Cluster of Differentiation 68; CD68
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD68 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD68 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD68 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD68 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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aaa15683 rat typical testing data standard curve for reference only aaa15683_td elisa kit cluster of differentiation 68 cd68 antigen partial 60653837 aax29611.1 samples serum plasma and tissue homogenates detection range 0.625 ng ml 40 sensitivity 0.156 wavelength 450 nm sample volume 50 100ul differentiation68 ml40 wavelength450 volume50
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Samples||Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate!!Assay Type||Competitive!!Detection Range||0.5-10ng/mL!!Sensitivity||0.1ng/mL
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Intended Uses: This TLR-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TLR-4. This ELISA kit for research use only!<br><br>Principle of the Assay: TLR-4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TLR-4. Standards or samples are then added to the microtiter plate wells and TLR-4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TLR-4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TLR-4 are added to each well to "sandwich" the TLR-4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TLR-4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLR-4 concentration in each sample is interpolated from this standard curve.
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aaa16601 mouse typical testing data standard curve for reference only aaa16601_sc elisa kit toll like receptor4 tlr4 partial 6,356 da receptor 4 d0el75_bubbu 260401829 acx37356.1 immunology samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type competitive detection range 0.5 10ng ml sensitivity 0.1ng range0.5
