Testing Data
(Anti-CD11b Picoband antibody, MBS177463-1.jpgAll lanes: Anti CD11B (MBS177463) at 0.5ug/mlWB: Recombinant Human CD11B Protein 0.5ngPredicted bind size: 45KDObserved bind size: 45KD )
Rabbit Integrin alpha-M Polyclonal Antibody | anti-ITGAM antibody
Anti-CD11b Antibody
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat
Immunohistochemistry (Frozen Section): Concentration: 0.5-1ug/ml; Tested Species:Human
Flow Cytometry: Concentration: 1-3ug/1x106; Tested Species: Human
Tested Species: In house tested species with positive test results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applicaitons have not been tested.
Optimal dilutions should be determined by end users.
Testing Data
(Anti-CD11b Picoband antibody, MBS177463-1.jpgAll lanes: Anti CD11B (MBS177463) at 0.5ug/mlWB: Recombinant Human CD11B Protein 0.5ngPredicted bind size: 45KDObserved bind size: 45KD )
Testing Data
(Anti-CD11b Picoband antibody, MBS177463-1.jpgAll lanes: Anti CD11B (MBS177463) at 0.5ug/mlWB: Recombinant Human CD11B Protein 0.5ngPredicted bind size: 45KDObserved bind size: 45KD )
Immunohistochemistry (IHC)
(Anti-CD11b Picoband antibody, MBS177463-2.JPGIHC(P): Human Tonsil Tissue )
Immunohistochemistry (IHC)
(Anti-CD11b Picoband antibody, MBS177463-2.JPGIHC(P): Human Tonsil Tissue )
Immunohistochemistry (IHC)
(Anti-CD11b Picoband antibody, MBS177463-3.JPGIHC(P): Mouse Spleen Tissue )
Immunohistochemistry (IHC)
(Anti-CD11b Picoband antibody, MBS177463-3.JPGIHC(P): Mouse Spleen Tissue )
Immunohistochemistry (IHC)
(Anti-CD11b Picoband antibody, MBS177463-4.JPGIHC(P): Rat Spleen Tissue )
Immunohistochemistry (IHC)
(Anti-CD11b Picoband antibody, MBS177463-4.JPGIHC(P): Rat Spleen Tissue )
Background: Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (alphaMbeta2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Uniprot Description
ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes.
Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: cell surface; plasma membrane; nucleus; integrin complex; external side of plasma membrane
Molecular Function: heparan sulfate proteoglycan binding; heparin binding; opsonin binding; protein binding; protein heterodimerization activity; metal ion binding; glycoprotein binding
Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; extracellular matrix organization and biogenesis; microglia development; cellular extravasation; activated T cell proliferation; toll-like receptor signaling pathway; innate immune response; cell adhesion; blood coagulation; toll-like receptor 4 signaling pathway; leukocyte migration; leukocyte migration during inflammatory response
Disease: Systemic Lupus Erythematosus, Susceptibility To, 6
Research Articles on ITGAM
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Product Notes
The ITGAM itgam (Catalog #AAA177463) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD11b Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin alpha-M can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC-P). Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat Immunohistochemistry (Frozen Section): Concentration: 0.5-1ug/ml; Tested Species:Human Flow Cytometry: Concentration: 1-3ug/1x106; Tested Species: Human Tested Species: In house tested species with positive test results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applicaitons have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the ITGAM itgam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin alpha-M, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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